Construct: sgRNA BRDN0001145444
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGCTTTGGCTATCGCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ALDH18A1 (5832)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77995
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
95616595 |
- |
ALDH18A1 |
NNNGCTTTGGCTATCGCAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
4104761 |
- |
ZZEF1 |
NNNGCTTTTGCTCTCGCAAG |
NGG |
2 |
0.1123 |
Tier I |
| 3 |
NC_000009.12 |
9 |
128262712 |
+ |
GOLGA2 |
NNNGCTTTGGCTATGGCCAG |
NGG |
2 |
0.0095 |
Tier I |
| 4 |
NC_000001.11 |
1 |
156643456 |
- |
BCAN |
NNNGCTTTGGCTATAGCAGG |
NGG |
2 |
0.0833 |
Tier II |
| 5 |
NC_000013.11 |
13 |
35897529 |
+ |
DCLK1 |
NNNGCTTGGGCTATGGCAAG |
NGG |
2 |
0.0367 |
Tier II |
| 6 |
NC_000011.10 |
11 |
22213877 |
+ |
ANO5 |
NNNGCTTTGGCTATTGGAAG |
NGG |
2 |
0.0039 |
Tier II |
| 7 |
NC_000015.10 |
15 |
68203930 |
+ |
CALML4 |
NNNGCTTTGGCTATCCCAGG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000020.11 |
20 |
30347269 |
- |
FAM242A |
NNNGCTTTGACTATCGCAAG |
NAG |
2 |
0.242 |
Tier III |
| 9 |
NC_000013.11 |
13 |
35897529 |
+ |
LOC105370163 |
NNNGCTTGGGCTATGGCAAG |
NGG |
2 |
0.0367 |
Tier III |
| 10 |
NC_000010.11 |
10 |
33051522 |
+ |
ITGB1-DT |
NNNGCTTTGGCTATTGGAAG |
NGG |
2 |
0.0039 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
115362316 |
- |
Hid1 |
NNNGCTTTGGCTATTGCATG |
NGG |
2 |
0.0359 |
Tier II |
| 2 |
NC_000067.6 |
1 |
83379738 |
- |
Sphkap |
NNNGCTTTGGCTTTGGCAAG |
NGG |
2 |
0.015 |
Tier II |
| 3 |
NC_000084.6 |
18 |
74635580 |
+ |
Myo5b |
NNNGCTTTGGCTAGCACAAG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
