Construct: sgRNA BRDN0001145448

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGGTGTAACAGGAACATCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACVR1 (90)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76757

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 157780361 - ACVR1 NNNGTGTAACAGGAACATCA NGG 0 1.0 Tier I
2 NC_000010.11 10 133402168 - MTG1 NNNGTCCAACAGGAACATCA NGG 2 0.4687 Tier I
3 NC_000012.12 12 72415326 + TRHDE NNNGTGTAATAGGAAAATCA NGG 2 0.9412 Tier II
4 NC_000003.12 3 159512654 + SCHIP1 NNNGTGAAACACGAACATCA NGG 2 0.4632 Tier II
5 NC_000003.12 3 159512654 + IQCJ-SCHIP1 NNNGTGAAACACGAACATCA NGG 2 0.4632 Tier II
6 NC_000001.11 1 248050427 + OR2L13 NNNTTGAAACAGGAACATCA NGG 2 0.3182 Tier II
7 NC_000001.11 1 248050427 + OR2L3 NNNTTGAAACAGGAACATCA NGG 2 0.3182 Tier II
8 NC_000009.12 9 5861802 + ERMP1 NNNGTGCAACAGCAACATCA NGG 2 0.2895 Tier II
9 NC_000011.10 11 40893444 - LRRC4C NNNGTGTAACAATAACATCA NGG 2 0.28 Tier II
10 NC_000001.11 1 21553459 - ALPL NNNGTGTCCCAGGAACATCA NGG 2 0.2449 Tier II
11 NC_000006.12 6 16681457 + ATXN1 NNNGTGTAACACGAACATTA NGG 2 0.2443 Tier II
12 NC_000002.12 2 152602912 - FMNL2 NNNGTGAAACAGGACCATCA NGG 2 0.2386 Tier II
13 NC_000023.11 X 112378489 + RTL4 NNNGTGTAACAGAAAGATCA NGG 2 0.142 Tier II
14 NC_000004.12 4 169703101 + CLCN3 NNNCTGTAACAGGAACATCA NAG 2 0.1373 Tier II
15 NC_000006.12 6 159760012 + SOD2 NNNGTGTAACAGGATCATCT NGG 2 0.12 Tier II
16 NC_000010.11 10 80151922 - ANXA11 NNNGTGTATCAGGATCATCA NGG 2 0.12 Tier II
17 NC_000013.11 13 46162187 - LCP1 NNNGTCTAACAGGAACATGA NGG 2 0.0852 Tier II
18 NC_000003.12 3 179818389 + PEX5L NNNGTGTAACAGTCACATCA NGG 2 0.0643 Tier II
19 NC_000007.14 7 147831928 - CNTNAP2 NNNGTGTAACAGTCACATCA NGG 2 0.0643 Tier II
20 NC_000014.9 14 105242210 - BRF1 NNNGTGTAACAGTCACATCA NGG 2 0.0643 Tier II
21 NC_000019.10 19 1874984 + KLF16 NNNATGTAACAGGAACATCA NGA 2 0.0625 Tier II
22 NC_000015.10 15 100650493 - ASB7 NNNGTGTAACATGAACATCA NTG 2 0.015 Tier II
23 NC_000011.10 11 11285039 + GALNT18 NNNGGGTAACAGGAACATCA NGT 2 0.0103 Tier II
24 NC_000008.11 8 131218638 + LOC105375760 NNNGGGTGACAGGAACATCA NGG 2 0.4693 Tier III
25 NC_000001.11 1 248050427 + LOC105373275 NNNTTGAAACAGGAACATCA NGG 2 0.3182 Tier III
26 NC_000005.10 5 85296844 + LOC101929321 NNNGTGTAATAGTAACATCA NGG 2 0.2824 Tier III
27 NC_000022.11 22 22741311 + IGL NNNGTGAAACAGTAACATCA NGG 2 0.2625 Tier III
28 NC_000007.14 7 25626779 + LINC03007 NNNGGGTAACAGGAACATCC NGG 2 0.1455 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 58479689 - Acvr1 NNNGTGTAACAGGAACATCA NGG 0 1.0 Tier I
2 NC_000071.6 5 120756253 - Oas3 NNNGTGTAACAAGACCATCA NGG 2 0.2545 Tier I
3 NC_000071.6 5 21616031 - Fbxl13 NNNGTGAAAAAGGAACATCA NGG 2 0.7583 Tier II
4 NC_000085.6 19 4884714 + Zdhhc24 NNNGTGAAAAAGGAACATCA NGG 2 0.7583 Tier II
5 NC_000071.6 5 88889638 - Slc4a4 NNNGTTTAAAAGGAACATCA NGG 2 0.5778 Tier II
6 NC_000076.6 10 108822233 - Syt1 NNNCTGTAACAAGAACATCA NGG 2 0.4941 Tier II
7 NC_000068.7 2 119140019 + Rmdn3 NNNGTGTGACAGGAACATCT NGG 2 0.44 Tier II
8 NC_000080.6 14 62902622 + Wdfy2 NNNGGGTAACAGGAGCATCA NGG 2 0.416 Tier II
9 NC_000073.6 7 132294581 - Chst15 NNNGTGTAACAGGAGCATCT NGG 2 0.39 Tier II
10 NC_000082.6 16 22389131 + Etv5 NNNGAGTGACAGGAACATCA NGG 2 0.3667 Tier II
11 NC_000070.6 4 36229469 - Lingo2 NNNCTGCAACAGGAACATCA NGG 2 0.364 Tier II
12 NC_000068.7 2 22763762 + Apbb1ip NNNGTGTAGCAGGAACAGCA NGG 2 0.2222 Tier II
13 NC_000071.6 5 89823211 + Adamts3 NNNGTATAACAGGAAGATCA NGG 2 0.1538 Tier II
14 NC_000081.6 15 37411554 - Ncald NNNGTGGAACAGGAACATCC NGG 2 0.1337 Tier II
15 NC_000073.6 7 86688758 + Olfr292 NNNGTGTAACAGCAATATCA NGG 2 0.1296 Tier II
16 NC_000068.7 2 122164103 - Patl2 NNNGTCTAACAGGAACATGA NGG 2 0.0852 Tier II
17 NC_000068.7 2 122164103 - Trim69 NNNGTCTAACAGGAACATGA NGG 2 0.0852 Tier II
18 NC_000068.7 2 6929248 - Celf2 NNNGTGTAACAGGAACATCC NCG 2 0.0244 Tier II
19 NC_000081.6 15 53090790 + Ext1 NNNGTGTAACAGGAACACCA NTG 2 0.0167 Tier II
20 NC_000071.6 5 99299740 + A930011G23Rik NNNATGTAACAGGAAAATCA NGG 2 0.9 Tier III
21 NC_000072.6 6 140680988 - Gm30215 NNNGTGTAGCAGGAACAACA NGG 2 0.4444 Tier III
22 NC_000082.6 16 89693824 - Gm35500 NNNGAGAAACAGGAACATCA NGG 2 0.4375 Tier III
23 NC_000072.6 6 118003400 - Gm38884 NNNTTGTAATAGGAACATCA NGG 2 0.3422 Tier III
24 NC_000076.6 10 3304801 - Gm29826 NNNGTGTAGCAGGAACAGCA NGG 2 0.2222 Tier III
25 NC_000085.6 19 19845772 - Gm32469 NNNGTGTAGCAGGAACAGCA NGG 2 0.2222 Tier III
26 NC_000072.6 6 31166048 - Lncpint NNNGTGTAGCAGGAACATCC NGG 2 0.1515 Tier III
27 NC_000069.6 3 73531435 - Gm20754 NNNGTGTAACAGGCACATCA NGA 2 0.0149 Tier III
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Other clones with same target sequence:

(none)