Construct: sgRNA BRDN0001145450
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGCTTCTGCATAAACGGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PBK (55872)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75572
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
27828160 |
+ |
PBK |
NNNCTTCTGCATAAACGGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
121267396 |
- |
GSN |
NNNCTTCTGCATAAACGGAA |
NGG |
1 |
0.9375 |
Tier II |
| 3 |
NC_000007.14 |
7 |
50338627 |
- |
IKZF1 |
NNNCTTTTGCATAAAAGGAG |
NGG |
2 |
0.8125 |
Tier II |
| 4 |
NC_000003.12 |
3 |
46872255 |
+ |
MYL3 |
NNNATTCTGCATAAACAGAG |
NGG |
2 |
0.786 |
Tier II |
| 5 |
NC_000001.11 |
1 |
182453726 |
+ |
RGSL1 |
NNNCTTCTTCATAAACGGAA |
NGG |
2 |
0.5 |
Tier II |
| 6 |
NC_000003.12 |
3 |
195144741 |
- |
XXYLT1 |
NNNCTTCTGCATAAAAGGAG |
NAG |
2 |
0.2593 |
Tier II |
| 7 |
NC_000012.12 |
12 |
2793357 |
+ |
ITFG2-AS1 |
NNNCTTCTGCATAAAAGTAG |
NGG |
2 |
0.6667 |
Tier III |
| 8 |
NC_000003.12 |
3 |
177490516 |
+ |
LINC00578 |
NNNCTTCTGCATAAACGAAG |
NGT |
2 |
0.0112 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
65811970 |
- |
Pbk |
NNNCTTCTGCATAAATGGAG |
NGG |
1 |
0.3077 |
Tier I |
| 2 |
NC_000075.6 |
9 |
35511536 |
+ |
Gm1113 |
NNNCTTCTGCAAAAACAGAG |
NGG |
2 |
0.7467 |
Tier II |
| 3 |
NC_000077.6 |
11 |
77107857 |
- |
Efcab5 |
NNNCTACTGCATAAACGGAT |
NGG |
2 |
0.6067 |
Tier II |
| 4 |
NC_000082.6 |
16 |
90133715 |
+ |
Tiam1 |
NNNCCTCTGCAGAAACGGAG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000081.6 |
15 |
95373753 |
+ |
Nell2 |
NNNCTTCTGCATAAACGAAC |
NGG |
2 |
0.2967 |
Tier II |
| 6 |
NC_000069.6 |
3 |
154052106 |
+ |
Slc44a5 |
NNNCTTATGCTTAAACGGAG |
NGG |
2 |
0.2308 |
Tier II |
| 7 |
NC_000067.6 |
1 |
175126021 |
+ |
Rgs7 |
NNNCTTCTGCATAAAAGGAG |
NGT |
2 |
0.0161 |
Tier II |
| 8 |
NC_000075.6 |
9 |
108294301 |
+ |
Nicn1 |
NNNCTTCTGCATAAAAGGAG |
NGT |
2 |
0.0161 |
Tier II |
| 9 |
NC_000069.6 |
3 |
17230642 |
- |
Gm5283 |
NNNCTTCCGCATAAACGGAT |
NGG |
2 |
0.7 |
Tier III |
| 10 |
NC_000077.6 |
11 |
77107857 |
- |
Gm12344 |
NNNCTACTGCATAAACGGAT |
NGG |
2 |
0.6067 |
Tier III |
| 11 |
NC_000070.6 |
4 |
39896066 |
+ |
Gm12387 |
NNNCTTCTGCATAAATGGAG |
NGG |
1 |
0.3077 |
Tier III |
Other clones with same target sequence:
(none)
