Construct: sgRNA BRDN0001145451
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCTACAGAGGGATACCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SGK494 (124923)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77172
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
28613111 |
- |
RSKR |
NNNTACAGAGGGATACCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
117299581 |
+ |
ASTN2 |
NNNTACAAAGGGATACAGTG |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000009.12 |
9 |
37831372 |
- |
DCAF10 |
NNNCACAGAGGGATACTGTG |
NGG |
2 |
0.302 |
Tier II |
| 4 |
NC_000009.12 |
9 |
133775267 |
+ |
VAV2 |
NNNTACAGTGGGATACAGTG |
NGG |
2 |
0.28 |
Tier II |
| 5 |
NC_000023.11 |
X |
80712180 |
- |
BRWD3 |
NNNATCAGAGGGATACCGTG |
NGG |
2 |
0.1732 |
Tier II |
| 6 |
NC_000008.11 |
8 |
73477701 |
+ |
STAU2 |
NNNTACAGAGGGATACCAGG |
NGG |
2 |
0.1731 |
Tier II |
| 7 |
NC_000016.10 |
16 |
72057013 |
- |
HP |
NNNTTCAGAGGGATACTGTG |
NGG |
2 |
0.1697 |
Tier II |
| 8 |
NC_000016.10 |
16 |
72058737 |
- |
HP |
NNNTTCAGAGGGATACTGTG |
NGG |
2 |
0.1697 |
Tier II |
| 9 |
NC_000017.11 |
17 |
28613111 |
- |
SPAG5-AS1 |
NNNTACAGAGGGATACCGTG |
NGG |
0 |
1.0 |
Tier III |
| 10 |
NC_000010.11 |
10 |
65789282 |
- |
LOC105378339 |
NNNTACAGAGGGATACACTG |
NGG |
2 |
0.2222 |
Tier III |
| 11 |
NC_000005.10 |
5 |
5374398 |
+ |
LOC101929200 |
NNNTACAGAGGGAGACCATG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
170046722 |
- |
Tshz2 |
NNNGACAGAGGGATACCTTG |
NGG |
2 |
0.4167 |
Tier II |
| 2 |
NC_000077.6 |
11 |
71227940 |
+ |
Nlrp1b |
NNNTACAGATGGATACAGTG |
NGG |
2 |
0.3792 |
Tier II |
| 3 |
NC_000081.6 |
15 |
94774314 |
- |
Tmem117 |
NNNTACAGAGGGATACTATG |
NGG |
2 |
0.3231 |
Tier II |
| 4 |
NC_000079.6 |
13 |
104095176 |
+ |
Nln |
NNNTCCAGAGGGATACAGTG |
NGG |
2 |
0.2333 |
Tier II |
| 5 |
NC_000067.6 |
1 |
85829027 |
+ |
Cab39 |
NNNTACAGAGTGATACAGTG |
NGG |
2 |
0.1795 |
Tier II |
| 6 |
NC_000075.6 |
9 |
79931609 |
+ |
Filip1 |
NNNTACAGAGGGCTACTGTG |
NGG |
2 |
0.0982 |
Tier II |
| 7 |
NC_000073.6 |
7 |
59925886 |
+ |
Snhg14 |
NNNTACAGAGGGATACATTG |
NGG |
2 |
0.3111 |
Tier III |
| 8 |
NC_000075.6 |
9 |
79931609 |
+ |
Gm35261 |
NNNTACAGAGGGCTACTGTG |
NGG |
2 |
0.0982 |
Tier III |
Other clones with same target sequence:
(none)
