Construct: sgRNA BRDN0001145453
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTCGGGCATGGTGCCCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HSPB8 (26353)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75640
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
119179522 |
+ |
HSPB8 |
NNNTCGGGCATGGTGCCCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
841833 |
- |
DMRT1 |
NNNTTGGGCATGGTGCCCCT |
NGG |
2 |
0.4455 |
Tier I |
| 3 |
NC_000019.10 |
19 |
58387401 |
+ |
RPS5 |
NNNTCGGGGATAGTGCCCCG |
NGG |
2 |
0.5778 |
Tier II |
| 4 |
NC_000001.11 |
1 |
7463179 |
- |
CAMTA1 |
NNNTAGGGCATGGAGCCCCG |
NGG |
2 |
0.3537 |
Tier II |
| 5 |
NC_000019.10 |
19 |
33770310 |
+ |
CHST8 |
NNNTCGGGCATGGTGCCCCG |
NCG |
1 |
0.1071 |
Tier II |
| 6 |
NC_000022.11 |
22 |
19811415 |
- |
GNB1L |
NNNTGGGGCATGGTGGCCCG |
NGG |
2 |
0.0923 |
Tier II |
| 7 |
NC_000007.14 |
7 |
66199863 |
- |
TPST1 |
NNNCCGGGCATGGTGCCGCG |
NGG |
2 |
0.0863 |
Tier II |
| 8 |
NC_000008.11 |
8 |
15441276 |
+ |
TUSC3 |
NNNTCGGGCATGGTGGCACG |
NGG |
2 |
0.0828 |
Tier II |
| 9 |
NC_000007.14 |
7 |
158444952 |
- |
PTPRN2 |
NNNTCTGGCATGGTGCCCCG |
NCG |
2 |
0.0714 |
Tier II |
| 10 |
NC_000010.11 |
10 |
63556265 |
- |
REEP3 |
NNNTCGGGCATGGTGGCCCG |
NGC |
2 |
0.0034 |
Tier II |
| 11 |
NC_000006.12 |
6 |
36196504 |
+ |
BRPF3-AS1 |
NNNCCGGGGATGGTGCCCCG |
NGG |
2 |
0.4006 |
Tier III |
| 12 |
NC_000008.11 |
8 |
15441276 |
+ |
LOC124902059 |
NNNTCGGGCATGGTGGCACG |
NGG |
2 |
0.0828 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
116422271 |
- |
Hspb8 |
NNNTCTGGCATGGTACCCCG |
NGG |
2 |
0.6275 |
Tier I |
| 2 |
NC_000074.6 |
8 |
104509440 |
- |
Terb1 |
NNNGCGTGCATGGTGCCCCG |
NGG |
2 |
0.3571 |
Tier II |
| 3 |
NC_000070.6 |
4 |
128799649 |
+ |
Zfp362 |
NNNTCGGGCATGGTGCTCTG |
NGG |
2 |
0.2154 |
Tier II |
Other clones with same target sequence:
(none)
