Construct: sgRNA BRDN0001145455
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTCGGAGGACTGCTCATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGGY (55277)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77111
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
59321702 |
- |
FGGY |
NNNCGGAGGACTGCTCATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
45914660 |
+ |
PEX16 |
NNNCGTAGGACTGCTCATGG |
NTG |
2 |
0.026 |
Tier I |
| 3 |
NC_000017.11 |
17 |
29835518 |
- |
SSH2 |
NNNCAGAAGACTGCTCATGG |
NGG |
2 |
0.8667 |
Tier II |
| 4 |
NC_000015.10 |
15 |
60534205 |
+ |
RORA |
NNNCGGAGGTCTGCTCATGA |
NGG |
2 |
0.8272 |
Tier II |
| 5 |
NC_000006.12 |
6 |
34814511 |
+ |
BLTP3A |
NNNAGGAGGACCGCTCATGG |
NGG |
2 |
0.7978 |
Tier II |
| 6 |
NC_000008.11 |
8 |
1232367 |
- |
DLGAP2 |
NNNCAGAGGACTGCTCATGT |
NGG |
2 |
0.6067 |
Tier II |
| 7 |
NC_000013.11 |
13 |
109011011 |
+ |
MYO16 |
NNNCAGAGGACTCCTCATGG |
NGG |
2 |
0.3649 |
Tier II |
| 8 |
NC_000017.11 |
17 |
46928513 |
+ |
GOSR2 |
NNNCCGAGGACTGATCATGG |
NGG |
2 |
0.275 |
Tier II |
| 9 |
NC_000017.11 |
17 |
46928513 |
+ |
LRRC37A2 |
NNNCCGAGGACTGATCATGG |
NGG |
2 |
0.275 |
Tier II |
| 10 |
NC_000012.12 |
12 |
24555041 |
+ |
SOX5 |
NNNCGAAGGACTGCTCATGG |
NAG |
2 |
0.2593 |
Tier II |
| 11 |
NC_000016.10 |
16 |
50680583 |
+ |
SNX20 |
NNNCGGAGGACTTTTCATGG |
NGG |
2 |
0.22 |
Tier II |
| 12 |
NC_000020.11 |
20 |
49674583 |
+ |
B4GALT5 |
NNNCGCAGGACTGCTCATGG |
NAG |
2 |
0.1768 |
Tier II |
| 13 |
NC_000002.12 |
2 |
130975882 |
+ |
ARHGEF4 |
NNNCGGAGGACTGCTGAGGG |
NGG |
2 |
0.0513 |
Tier II |
| 14 |
NC_000023.11 |
X |
153495634 |
- |
BGN |
NNNCGGAGGACTGCTCCTGG |
NTG |
2 |
0.0069 |
Tier II |
| 15 |
NC_000006.12 |
6 |
45078164 |
- |
SUPT3H |
NNNCGGAGGACTGGACATGG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000017.11 |
17 |
29835518 |
- |
LOC124900390 |
NNNCAGAAGACTGCTCATGG |
NGG |
2 |
0.8667 |
Tier III |
| 17 |
NC_000015.10 |
15 |
60534205 |
+ |
RORA-AS1 |
NNNCGGAGGTCTGCTCATGA |
NGG |
2 |
0.8272 |
Tier III |
| 18 |
NC_000011.10 |
11 |
76615454 |
- |
LINC02757 |
NNNAGGAGGCCTGCTCATGG |
NGG |
2 |
0.2807 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
95585399 |
- |
Fggy |
NNNCTGAGGACTGCTCGTGG |
NGG |
2 |
0.0529 |
Tier I |
| 2 |
NC_000067.6 |
1 |
180178977 |
+ |
Coq8a |
NNNCGGAGGACTGGTCCTGG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000076.6 |
10 |
40688764 |
+ |
Mettl24 |
NNNCAGAGGACTGCTAATGG |
NGG |
2 |
0.8667 |
Tier II |
| 4 |
NC_000071.6 |
5 |
130824828 |
+ |
Caln1 |
NNNTGGAGGACAGCTCATGG |
NGG |
2 |
0.64 |
Tier II |
| 5 |
NC_000087.7 |
Y |
1444947 |
+ |
Usp9y |
NNNCGGTGGAATGCTCATGG |
NGG |
2 |
0.3281 |
Tier II |
| 6 |
NC_000082.6 |
16 |
17573256 |
- |
Slc7a4 |
NNNCGGAGGCTTGCTCATGG |
NGG |
2 |
0.1026 |
Tier II |
| 7 |
NC_000069.6 |
3 |
35789967 |
+ |
Atp11b |
NNNCTGAGGACTGCTCTTGG |
NGG |
2 |
0.04 |
Tier II |
| 8 |
NC_000070.6 |
4 |
103089040 |
- |
Wdr78 |
NNNCTGAGGACTGCTCATGG |
NCG |
2 |
0.0321 |
Tier II |
| 9 |
NC_000070.6 |
4 |
103439276 |
+ |
Gm12718 |
NNNTGGAGAACTGCTCATGG |
NGG |
2 |
0.5143 |
Tier III |
Other clones with same target sequence:
(none)
