Construct: sgRNA BRDN0001145457
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTGCGTCAGCACCGTAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IP6K2 (51447)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77818
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
48692973 |
- |
IP6K2 |
NNNGCGTCAGCACCGTAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
146962998 |
- |
PPP2R2B |
NNNGAGTCAGCACAGTAAAG |
NGG |
2 |
0.2 |
Tier II |
| 3 |
NC_000004.12 |
4 |
26809851 |
- |
TBC1D19 |
NNNGAGTCAGGACCGTAAAG |
NGG |
2 |
0.1429 |
Tier II |
| 4 |
NC_000001.11 |
1 |
121239555 |
+ |
SRGAP2C |
NNNGCGTAAGCACCGTAAAG |
NTG |
2 |
0.0253 |
Tier II |
| 5 |
NC_000001.11 |
1 |
145040811 |
- |
SRGAP2B |
NNNGCGTAAGCACCGTAAAG |
NTG |
2 |
0.0253 |
Tier II |
| 6 |
NC_000006.12 |
6 |
75637137 |
+ |
SENP6 |
NNNGCCTCAGCACCGTAAAG |
NGC |
2 |
0.0152 |
Tier II |
| 7 |
NC_000003.12 |
3 |
9843153 |
+ |
RPUSD3 |
NNNGCGTCAGCACCCTAAAG |
NGC |
2 |
0.0061 |
Tier II |
| 8 |
NC_000001.11 |
1 |
144027141 |
+ |
SRGAP2D |
NNNGCGTAAGCACCGTAAAG |
NTG |
2 |
0.0253 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
97418393 |
- |
Frmd4b |
NNNGCGTCAGCACTGGAAAG |
NGG |
2 |
0.2538 |
Tier II |
| 2 |
NC_000080.6 |
14 |
70329877 |
+ |
Slc39a14 |
NNNGCGTCAGCACCGTGGAG |
NGG |
2 |
0.0706 |
Tier II |
Other clones with same target sequence:
(none)
