Construct: sgRNA BRDN0001145459
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTACCCCCAGAATTCCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BAZ1B (9031)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76133
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
73478098 |
+ |
BAZ1B |
NNNACCCCCAGAATTCCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
25476589 |
- |
DTNB |
NNNACCCCTAGAATTCCATG |
NGG |
2 |
0.6058 |
Tier II |
| 3 |
NC_000012.12 |
12 |
113943464 |
+ |
RBM19 |
NNNACACACAGAATTCCGTG |
NGG |
2 |
0.6036 |
Tier II |
| 4 |
NC_000013.11 |
13 |
113151286 |
+ |
LOC102724474 |
NNNACCCCCAGAATTCCCTG |
NGG |
1 |
0.4762 |
Tier II |
| 5 |
NC_000001.11 |
1 |
12590176 |
- |
DHRS3 |
NNNACCCCCAGAGTTCCTTG |
NGG |
2 |
0.4348 |
Tier II |
| 6 |
NC_000016.10 |
16 |
13437672 |
- |
SHISA9 |
NNNGCCCCCAGAATTCAGTG |
NGG |
2 |
0.2917 |
Tier II |
| 7 |
NC_000004.12 |
4 |
151443774 |
+ |
FHIP1A |
NNNACCCCCAAAATGCCGTG |
NGG |
2 |
0.05 |
Tier II |
| 8 |
NC_000002.12 |
2 |
159355034 |
- |
BAZ2B |
NNNACCCCCAGAATCCCGTG |
NGA |
2 |
0.0189 |
Tier II |
| 9 |
NC_000001.11 |
1 |
15784898 |
+ |
FBLIM1 |
NNNACCCCCAGAATTCCCTG |
NTG |
2 |
0.0186 |
Tier II |
| 10 |
NC_000004.12 |
4 |
111952128 |
+ |
LINC02945 |
NNNACCCCCAGAATTCCTTG |
NGG |
1 |
0.6667 |
Tier III |
| 11 |
NC_000001.11 |
1 |
67864370 |
+ |
GNG12-AS1 |
NNNACCCCAAGAATTCCATG |
NGG |
2 |
0.5934 |
Tier III |
| 12 |
NC_000006.12 |
6 |
25124005 |
- |
CMAHP |
NNNACTCCCAGAATTCAGTG |
NGG |
2 |
0.4333 |
Tier III |
| 13 |
NC_000006.12 |
6 |
25124005 |
- |
LOC124901282 |
NNNACTCCCAGAATTCAGTG |
NGG |
2 |
0.4333 |
Tier III |
| 14 |
NC_000001.11 |
1 |
27039927 |
+ |
LOC101928391 |
NNNAGCCCCAGAATTCCCTG |
NGG |
2 |
0.2857 |
Tier III |
| 15 |
NC_000005.10 |
5 |
176664848 |
+ |
LOC124901144 |
NNNACCCCCAGCATTCAGTG |
NGG |
2 |
0.1228 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
135217065 |
- |
Baz1b |
NNNACCCCCAGAACTCCGTG |
NGG |
1 |
0.2857 |
Tier I |
| 2 |
NC_000070.6 |
4 |
36618812 |
+ |
Lingo2 |
NNNACACCCAGAATTCCTTG |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000077.6 |
11 |
68244134 |
- |
Ntn1 |
NNNACTCCCAGAATTCCTTG |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000071.6 |
5 |
97388638 |
+ |
Naa11 |
NNNACTCCCAGAATTCCCTG |
NGG |
2 |
0.4422 |
Tier II |
| 5 |
NC_000076.6 |
10 |
74605152 |
+ |
Pcdh15 |
NNNAACCCCAGAATTCCTTG |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000074.6 |
8 |
94682315 |
+ |
Pllp |
NNNAACCCCAGAGTTCCGTG |
NGG |
2 |
0.3727 |
Tier II |
| 7 |
NC_000077.6 |
11 |
95387462 |
+ |
Slc35b1 |
NNNACACCCAGAATCCCGTG |
NGG |
2 |
0.2532 |
Tier II |
| 8 |
NC_000084.6 |
18 |
4977165 |
+ |
Svil |
NNNACCCCCAGAATTCACTG |
NGG |
2 |
0.2222 |
Tier II |
| 9 |
NC_000067.6 |
1 |
8903125 |
+ |
Sntg1 |
NNNACCCCCAGAATTCCTTG |
NAG |
2 |
0.1728 |
Tier II |
| 10 |
NC_000073.6 |
7 |
122748958 |
- |
Cacng3 |
NNNACCCCCAGAACTCCCTG |
NGG |
2 |
0.1361 |
Tier II |
| 11 |
NC_000070.6 |
4 |
76467650 |
- |
Ptprd |
NNNACCCCCAGAATTCCCTG |
NGA |
2 |
0.0331 |
Tier II |
| 12 |
NC_000086.7 |
X |
168444801 |
+ |
Frmpd4 |
NNNACCCCCAGAATTCCCTG |
NGA |
2 |
0.0331 |
Tier II |
| 13 |
NC_000067.6 |
1 |
167772490 |
- |
Lmx1a |
NNNACCCCCAGAACTCCGTG |
NGA |
2 |
0.0198 |
Tier II |
| 14 |
NC_000073.6 |
7 |
86895011 |
+ |
Gm39047 |
NNNACTCCCAGAATTCCTTG |
NGG |
2 |
0.619 |
Tier III |
| 15 |
NC_000069.6 |
3 |
18508956 |
+ |
Gm6369 |
NNNACCCCCAGAATTCCCTG |
NGA |
2 |
0.0331 |
Tier III |
| 16 |
NC_000070.6 |
4 |
76467650 |
- |
Gm42303 |
NNNACCCCCAGAATTCCCTG |
NGA |
2 |
0.0331 |
Tier III |
Other clones with same target sequence:
(none)
