Construct: sgRNA BRDN0001145466
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACCGGACATTGCAATAGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKK (139189)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75843
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
50470139 |
+ |
DGKK |
NNNGGACATTGCAATAGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
35855218 |
+ |
HEATR9 |
NNNGGACTTTGCAATAAACA |
NGG |
2 |
0.4 |
Tier I |
| 3 |
NC_000007.14 |
7 |
103640555 |
- |
RELN |
NNNGGACAGTGCACTAGACA |
NGG |
2 |
0.1327 |
Tier I |
| 4 |
NC_000003.12 |
3 |
41569032 |
+ |
ULK4 |
NNNGGACATTACAAAAGACA |
NGG |
2 |
0.5789 |
Tier II |
| 5 |
NC_000010.11 |
10 |
18571570 |
- |
NSUN6 |
NNNGGACATTGGAATAGACA |
NGG |
1 |
0.4444 |
Tier II |
| 6 |
NC_000007.14 |
7 |
31817196 |
+ |
PDE1C |
NNNGGAAATTGCAGTAGACA |
NGG |
2 |
0.35 |
Tier II |
| 7 |
NC_000009.12 |
9 |
122058332 |
+ |
TTLL11 |
NNNGGGCTTTGCAATAGACA |
NGG |
2 |
0.3061 |
Tier II |
| 8 |
NC_000012.12 |
12 |
44375527 |
+ |
TMEM117 |
NNNGGACAGTGGAATAGACA |
NGG |
2 |
0.2751 |
Tier II |
| 9 |
NC_000007.14 |
7 |
132168016 |
+ |
PLXNA4 |
NNNGGAGATTGCAGTAGACA |
NGG |
2 |
0.2196 |
Tier II |
| 10 |
NC_000018.10 |
18 |
58565617 |
- |
ALPK2 |
NNNGGACATTGTAATAGACA |
NAG |
2 |
0.1396 |
Tier II |
| 11 |
NC_000015.10 |
15 |
90026310 |
+ |
ZNF710 |
NNNGGACATTGCAATAGATA |
NAG |
2 |
0.1197 |
Tier II |
| 12 |
NC_000012.12 |
12 |
71982522 |
- |
TPH2 |
NNNGGACTTTGCAATATACA |
NGG |
2 |
0.1071 |
Tier II |
| 13 |
NC_000012.12 |
12 |
69946979 |
- |
MYRFL |
NNNGGACATTGCAATGGACA |
NAG |
2 |
0.0499 |
Tier II |
| 14 |
NC_000013.11 |
13 |
112183474 |
+ |
LOC105370370 |
NNNGGAAATTGCAAAAGACA |
NGG |
2 |
0.4342 |
Tier III |
| 15 |
NC_000015.10 |
15 |
100738448 |
+ |
LOC105371024 |
NNNGGACATTGCATTAGTCA |
NGG |
2 |
0.2667 |
Tier III |
| 16 |
NC_000018.10 |
18 |
58565617 |
- |
LOC105372145 |
NNNGGACATTGTAATAGACA |
NAG |
2 |
0.1396 |
Tier III |
| 17 |
NC_000015.10 |
15 |
97840006 |
- |
LINC00923 |
NNNCGACATTGCAATATACA |
NGG |
2 |
0.1324 |
Tier III |
| 18 |
NC_000012.12 |
12 |
115737028 |
- |
LOC105370003 |
NNNGGACATTGCAATAGTCC |
NGG |
2 |
0.1136 |
Tier III |
| 19 |
NC_000001.11 |
1 |
80090655 |
+ |
LOC553139 |
NNNGGACATTGCCATACACA |
NGG |
2 |
0.0495 |
Tier III |
| 20 |
NC_000001.11 |
1 |
165573183 |
+ |
LRRC52-AS1 |
NNNGGACATTGCAATAGCCA |
NGT |
2 |
0.0031 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
141992091 |
- |
Bmpr1b |
NNNGGACAGTGCAATAAACA |
NGG |
2 |
0.5778 |
Tier II |
| 2 |
NC_000080.6 |
14 |
60816934 |
- |
Mipep |
NNNAGACATTGCTATAGACA |
NGG |
2 |
0.27 |
Tier II |
| 3 |
NC_000074.6 |
8 |
23088206 |
- |
Ank1 |
NNNGGAGATTGTAATAGACA |
NGG |
2 |
0.2534 |
Tier II |
| 4 |
NC_000068.7 |
2 |
155029717 |
+ |
a |
NNNGGACATGGCACTAGACA |
NGG |
2 |
0.1071 |
Tier II |
| 5 |
NC_000077.6 |
11 |
5570895 |
- |
Ankrd36 |
NNNGGGCATTGCAAGAGACA |
NGG |
2 |
0.0357 |
Tier II |
| 6 |
NC_000071.6 |
5 |
112475827 |
+ |
Sez6l |
NNNGGACATTGCAGGAGACA |
NGG |
2 |
0.0233 |
Tier II |
| 7 |
NC_000070.6 |
4 |
139806695 |
- |
Pax7 |
NNNGGACACTGCAATAGACA |
NGT |
2 |
0.0149 |
Tier II |
| 8 |
NC_000071.6 |
5 |
118262903 |
+ |
Spring1 |
NNNGGACATTGCAAGAGACA |
NGC |
2 |
0.0011 |
Tier II |
| 9 |
NC_000079.6 |
13 |
50342186 |
- |
Gm46385 |
NNNGGACATAGCAATGGACA |
NGG |
2 |
0.1648 |
Tier III |
| 10 |
NC_000079.6 |
13 |
50885963 |
+ |
Gm8777 |
NNNGGACATAGCAATGGACA |
NGG |
2 |
0.1648 |
Tier III |
| 11 |
NC_000080.6 |
14 |
43335221 |
- |
Gm9597 |
NNNGGAAATTGCAATAGAGA |
NGG |
2 |
0.0938 |
Tier III |
Other clones with same target sequence:
(none)
