Construct: sgRNA BRDN0001145473

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAGCATTAAAGACGACTCGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKCZ (5590)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76631

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 2148920 + PRKCZ NNNCATTAAAGACGACTCGG NGG 0 1.0 Tier I
2 NC_000016.10 16 75109565 + ZNRF1 NNNCACTAAAGAAGACTCGG NGG 2 0.3497 Tier II
3 NC_000011.10 11 3888856 - STIM1 NNNCATTAAAGACTACACGG NGG 2 0.1422 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 47866185 + Sgcd NNNCAGAAAAGACGACTCGG NGG 2 0.5 Tier II
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Other clones with same target sequence:

(none)