Construct: sgRNA BRDN0001145479
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCCGGGGAGTATAGCTGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- OBSCN (84033)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76114
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
228224690 |
+ |
OBSCN |
NNNCGGGGAGTATAGCTGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
228250196 |
+ |
OBSCN |
NNNCGGGGAGTATAGCTGCG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000001.11 |
1 |
228256882 |
+ |
OBSCN |
NNNCGGGGAGTACAGCTGCG |
NGG |
1 |
0.7895 |
Tier I |
| 4 |
NC_000001.11 |
1 |
228268761 |
+ |
OBSCN |
NNNCGGGGAGTACAGCTGCG |
NGG |
1 |
0.7895 |
Tier I |
| 5 |
NC_000001.11 |
1 |
228272177 |
+ |
OBSCN |
NNNCGGGGAGTACAGCTGCG |
NGG |
1 |
0.7895 |
Tier I |
| 6 |
NC_000001.11 |
1 |
228264347 |
+ |
OBSCN |
NNNCGGGGAGTACAGCTGCA |
NGG |
2 |
0.7401 |
Tier I |
| 7 |
NC_000001.11 |
1 |
228245644 |
+ |
OBSCN |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 8 |
NC_000001.11 |
1 |
228259732 |
+ |
OBSCN |
NNNCGGAGAGTATAGCTGTG |
NGG |
2 |
0.4615 |
Tier I |
| 9 |
NC_000001.11 |
1 |
228243414 |
+ |
OBSCN |
NNNCGGGGACTACAGCTGCG |
NGG |
2 |
0.3158 |
Tier I |
| 10 |
NC_000019.10 |
19 |
1621049 |
+ |
TCF3 |
NNNCGGGGAGTAGATCTGCG |
NGG |
2 |
0.0373 |
Tier I |
| 11 |
NC_000006.12 |
6 |
119126507 |
+ |
FAM184A |
NNNCGGGGAGTGTAGCTGAG |
NGG |
2 |
0.3095 |
Tier II |
| 12 |
NC_000004.12 |
4 |
47751285 |
+ |
CORIN |
NNNCGGGGAGTATAGCTCAG |
NGG |
2 |
0.2041 |
Tier II |
| 13 |
NC_000001.11 |
1 |
1091148 |
- |
C1orf159 |
NNNCGGGGAGTGTAGCTGCG |
NCG |
2 |
0.0774 |
Tier II |
| 14 |
NC_000002.12 |
2 |
98635861 |
- |
MGAT4A |
NNNCTGGGAGTATAGGTGCG |
NGG |
2 |
0.0462 |
Tier II |
| 15 |
NC_000001.11 |
1 |
228268761 |
+ |
LOC124904535 |
NNNCGGGGAGTACAGCTGCG |
NGG |
1 |
0.7895 |
Tier III |
| 16 |
NC_000001.11 |
1 |
228272177 |
+ |
LOC124904535 |
NNNCGGGGAGTACAGCTGCG |
NGG |
1 |
0.7895 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
59085110 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 2 |
NC_000077.6 |
11 |
59086710 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 3 |
NC_000077.6 |
11 |
59090553 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 4 |
NC_000077.6 |
11 |
59093341 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 5 |
NC_000077.6 |
11 |
59099735 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 6 |
NC_000077.6 |
11 |
59103320 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 7 |
NC_000077.6 |
11 |
59106340 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 8 |
NC_000077.6 |
11 |
59107787 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 9 |
NC_000077.6 |
11 |
59112564 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 10 |
NC_000077.6 |
11 |
59115704 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 11 |
NC_000077.6 |
11 |
59122665 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 12 |
NC_000077.6 |
11 |
59124566 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 13 |
NC_000077.6 |
11 |
59128108 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 14 |
NC_000077.6 |
11 |
59129615 |
- |
Obscn |
NNNTGGGGAGTACAGCTGCG |
NGG |
2 |
0.6316 |
Tier I |
| 15 |
NC_000078.6 |
12 |
71958259 |
+ |
Daam1 |
NNNCGGGGAGTATAGCTCAG |
NGG |
2 |
0.2041 |
Tier II |
| 16 |
NC_000083.6 |
17 |
86565326 |
+ |
Prkce |
NNNCGGGGACTATAGCTCCG |
NGG |
2 |
0.1905 |
Tier II |
Other clones with same target sequence:
(none)
