Construct: sgRNA BRDN0001145480
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCCACCCCCAGGTTCTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCZ (5590)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76633
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
2172060 |
+ |
PRKCZ |
NNNCACCCCCAGGTTCTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
603549 |
+ |
HCN2 |
NNNCGCCCCCAGGTTCTACT |
NGG |
2 |
0.504 |
Tier I |
| 3 |
NC_000020.11 |
20 |
44750315 |
- |
KCNK15 |
NNNCACCACCAGGTTCTCCG |
NGG |
2 |
0.1238 |
Tier I |
| 4 |
NC_000016.10 |
16 |
57723126 |
+ |
DRC7 |
NNNCACCCCCAGGCTCTGCG |
NGG |
2 |
0.1143 |
Tier I |
| 5 |
NC_000007.14 |
7 |
64500739 |
- |
ZNF680 |
NNNTACTCCCAGGTTCTACG |
NGG |
2 |
0.65 |
Tier II |
| 6 |
NC_000022.11 |
22 |
29908127 |
- |
MTMR3 |
NNNCACCCCCAGGTTTTACA |
NGG |
2 |
0.2885 |
Tier II |
| 7 |
NC_000009.12 |
9 |
132070843 |
+ |
MED27 |
NNNCACCCCCAGGTTCTGTG |
NGG |
2 |
0.1846 |
Tier II |
| 8 |
NC_000019.10 |
19 |
16896724 |
- |
CPAMD8 |
NNNCACCCCCAGGTTCTGTG |
NGG |
2 |
0.1846 |
Tier II |
| 9 |
NC_000007.14 |
7 |
42230843 |
+ |
GLI3 |
NNNCACCCCCACTTTCTACG |
NGG |
2 |
0.1588 |
Tier II |
| 10 |
NC_000006.12 |
6 |
126249671 |
- |
TRMT11 |
NNNCACCCCCAGGCTCTAAG |
NGG |
2 |
0.1224 |
Tier II |
| 11 |
NC_000017.11 |
17 |
7414088 |
+ |
NLGN2 |
NNNCACCCCCAGCCTCTACG |
NGG |
2 |
0.1203 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
8068191 |
- |
Slc22a28 |
NNNCACCACCAGGTTCTACT |
NGG |
2 |
0.455 |
Tier II |
| 2 |
NC_000077.6 |
11 |
121572442 |
- |
Tbcd |
NNNCACCCTCAGGTTCTATG |
NGG |
2 |
0.4038 |
Tier II |
| 3 |
NC_000082.6 |
16 |
4553550 |
- |
Tfap4 |
NNNCACTCCCAGGTTCTATG |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000069.6 |
3 |
95285884 |
+ |
Mindy1 |
NNNCAGCCCCAGGTTCCACG |
NGG |
2 |
0.3529 |
Tier II |
| 5 |
NC_000068.7 |
2 |
24078174 |
- |
Tbpl2 |
NNNCACCACCAGGTTCTACC |
NGG |
2 |
0.2786 |
Tier II |
| 6 |
NC_000069.6 |
3 |
64112306 |
- |
Vmn2r1 |
NNNCACCACCAGGTTCTACC |
NGG |
2 |
0.2786 |
Tier II |
| 7 |
NC_000069.6 |
3 |
64114566 |
+ |
Vmn2r1 |
NNNCACCACCAGGTTCTACC |
NGG |
2 |
0.2786 |
Tier II |
| 8 |
NC_000071.6 |
5 |
128880634 |
+ |
Rimbp2 |
NNNCACCACCAGGTTCTACC |
NGG |
2 |
0.2786 |
Tier II |
| 9 |
NC_000076.6 |
10 |
114632362 |
+ |
Trhde |
NNNCACCACCAGGTTCTACC |
NGG |
2 |
0.2786 |
Tier II |
| 10 |
NC_000086.7 |
X |
69708697 |
- |
Aff2 |
NNNCACCACCAGGTTCTACC |
NGG |
2 |
0.2786 |
Tier II |
| 11 |
NC_000073.6 |
7 |
139994964 |
+ |
Adam8 |
NNNCACCCCCAGGCTCTACA |
NGG |
2 |
0.2679 |
Tier II |
| 12 |
NC_000068.7 |
2 |
142663466 |
+ |
Kif16b |
NNNCCCCCCCAGCTTCTACG |
NGG |
2 |
0.2105 |
Tier II |
| 13 |
NC_000086.7 |
X |
144698562 |
- |
Rtl4 |
NNNCACCCCCAGCTTCTATG |
NGG |
2 |
0.1943 |
Tier II |
| 14 |
NC_000069.6 |
3 |
25125848 |
+ |
Naaladl2 |
NNNCACCACCAGGTCCTACG |
NGG |
2 |
0.1773 |
Tier II |
| 15 |
NC_000083.6 |
17 |
58284334 |
- |
Cntnap5c |
NNNCACCACCAGGTCCTACG |
NGG |
2 |
0.1773 |
Tier II |
| 16 |
NC_000084.6 |
18 |
35236199 |
+ |
Ctnna1 |
NNNCACCCCCAGGACCTACG |
NGG |
2 |
0.1688 |
Tier II |
| 17 |
NC_000076.6 |
10 |
13189295 |
- |
Ltv1 |
NNNCACCCCCTGTTTCTACG |
NGG |
2 |
0.0923 |
Tier II |
| 18 |
NC_000071.6 |
5 |
60994731 |
- |
Gm40307 |
NNNCACCACCAGGTTCTACC |
NGG |
2 |
0.2786 |
Tier III |
| 19 |
NC_000082.6 |
16 |
42800605 |
+ |
4932412D23Rik |
NNNCACCACCAGGTTCTACC |
NGG |
2 |
0.2786 |
Tier III |
Other clones with same target sequence:
(none)
