Construct: sgRNA BRDN0001145482

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGAACTGCAATCTTATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80250

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 173507047 - SLC9C2 NNNAAATGCAATTTTATTAT NGG 2 0.65 Tier I
2 NC_000011.10 11 18396904 - LDHA NNNAACTGTAATCTTATTCT NGG 2 0.181 Tier I
3 NC_000011.10 11 93697665 - CEP295 NNNAACTGCAATATTATTCT NGG 2 0.0796 Tier I
4 NC_000018.10 18 56681085 + WDR7 NNNAAATGCAGTCTTATTAT NGG 2 0.6036 Tier II
5 NC_000011.10 11 131710507 + NTM NNNTACTGAAATCTTATTAT NGG 2 0.5455 Tier II
6 NC_000003.12 3 69809150 - MITF NNNAACTGTAATATTATTAT NGG 2 0.3365 Tier II
7 NC_000006.12 6 151382223 + ZBTB2 NNNCACTGAAATCTTATTAT NGG 2 0.3025 Tier II
8 NC_000005.10 5 22459319 - CDH12 NNNAACTGCAATCTTAATTT NGG 2 0.2872 Tier II
9 NC_000004.12 4 98633420 - TSPAN5 NNNATCTGCAATTTTATTAT NGG 2 0.2545 Tier II
10 NC_000012.12 12 12180220 - LRP6 NNNAACTGTAATCTCATTAT NGG 2 0.2386 Tier II
11 NC_000010.11 10 79837249 + NUTM2E NNNAACTGCAATCTTATTTT NAG 2 0.1396 Tier II
12 NC_000023.11 X 85142543 + SATL1 NNNAACTGCAATTTTATTAG NGG 2 0.1235 Tier II
13 NC_000007.14 7 137534294 - DGKI NNNAACTGCAATGTTATTAA NGG 2 0.0767 Tier II
14 NC_000007.14 7 28090367 + JAZF1 NNNAACTGCAATCTTAATAC NGG 2 0.0485 Tier II
15 NC_000005.10 5 90701856 + ADGRV1 NNNAACTGCAATTTTATTAT NGT 2 0.0113 Tier II
16 NC_000013.11 13 99538459 - TM9SF2 NNNAACTGCCATCTTATTAT NGC 2 0.0074 Tier II
17 NC_000001.11 1 76542841 - ST6GALNAC3 NNNAACTGCAATCTTCTAAT NGG 2 0.0 Tier II
18 NC_000011.10 11 61074328 - LOC105369325 NNNAACTATAATCTTATTAT NGG 2 0.875 Tier III
19 NC_000011.10 11 61074328 - LOC124902677 NNNAACTATAATCTTATTAT NGG 2 0.875 Tier III
20 NC_000022.11 22 22390593 + IGL NNNAACTGCAACCTTACTAT NGG 2 0.6687 Tier III
21 NC_000005.10 5 152879480 + LINC01470 NNNACCAGCAATCTTATTAT NGG 2 0.4375 Tier III
22 NC_000014.9 14 59000937 + LINC01500 NNNAACTGGAATTTTATTAT NGG 2 0.4333 Tier III
23 NC_000009.12 9 14922274 + LDHAP4 NNNAACTGTAATCTTATTCT NGG 2 0.181 Tier III
24 NC_000016.10 16 53518579 - LOC105371268 NNNAACTGCATTCTTATTAT NAG 2 0.0798 Tier III
25 NC_000016.10 16 79802860 - LINC01228 NNNAACTGCAATCTTCTTCT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 110184417 + Smarcc1 NNNAATTGCAAGCTTATTAT NGG 2 0.4643 Tier II
2 NC_000074.6 8 61529082 + Palld NNNAACTGCAGTCTTACTAT NGG 2 0.4588 Tier II
3 NC_000067.6 1 125987858 + Nckap5 NNNAACAGCCATCTTATTAT NGG 2 0.2917 Tier II
4 NC_000068.7 2 41187124 + Lrp1b NNNAACTGAATTCTTATTAT NGG 2 0.2637 Tier II
5 NC_000084.6 18 82941057 + Zfp516 NNNAACTGCAAGCTTATCAT NGG 2 0.2143 Tier II
6 NC_000068.7 2 140538517 - Macrod2 NNNAACTGCAATCCAATTAT NGG 2 0.1654 Tier II
7 NC_000069.6 3 59875328 - Aadacl2fm3 NNNAAGTGCAATCTTATTCT NGG 2 0.1034 Tier II
8 NC_000079.6 13 40231786 + Ofcc1 NNNAACTGCATTCTTATTAG NGG 2 0.0543 Tier II
9 NC_000073.6 7 125801487 + D430042O09Rik NNNACCTGCAATCTTATTAT NTG 2 0.0195 Tier II
10 NC_000083.6 17 11799066 + Prkn NNNAACTGCCATCTTCTTAT NGG 2 0.0 Tier II
11 NC_000075.6 9 66493787 - Herc1 NNNAACTGCCATCTTTTTAT NGG 2 0.0 Tier II
12 NC_000075.6 9 63344847 + Map2k5 NNNAACTGGAATCTTTTTAT NGG 2 0.0 Tier II
13 NC_000084.6 18 6291015 - Gm41664 NNNAACTGCTATCATATTAT NGG 2 0.5462 Tier III
14 NC_000084.6 18 35002328 + Gm36037 NNNAGCTTCAATCTTATTAT NGG 2 0.45 Tier III
15 NC_000078.6 12 63073081 + Gm5185 NNNAACTGTAATCTTATTCT NGG 2 0.181 Tier III
16 NC_000073.6 7 59622731 + Ipw NNNAACTGCACTATTATTAT NGG 2 0.1538 Tier III
17 NC_000073.6 7 59622731 + Snhg14 NNNAACTGCACTATTATTAT NGG 2 0.1538 Tier III
18 NC_000067.6 1 77827134 + Gm33030 NNNAACTGCAATCTTAGTAG NGG 2 0.0208 Tier III
19 NC_000067.6 1 77827134 + Gm33083 NNNAACTGCAATCTTAGTAG NGG 2 0.0208 Tier III
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Other clones with same target sequence:

(none)