Construct: sgRNA BRDN0001145484

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TATTCCCATTGGCATCCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUAK2 (81788)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77000

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 205308187 + NUAK2 NNNTCCCATTGGCATCCAAG NGG 0 1.0 Tier I
2 NC_000019.10 19 53050808 - ERVV-2 NNNGCCCACTGGCATCCAAG NGG 2 0.5769 Tier I
3 NC_000005.10 5 141956185 + PCDH12 NNNTATCATTGGCATCCAAG NGG 2 0.5306 Tier I
4 NC_000006.12 6 169223349 + THBS2 NNNTCCCTTTGGGATCCAAG NGG 2 0.0584 Tier I
5 NC_000018.10 18 53402871 - DCC NNNTCCCATTGGCTTCCAAG NGA 2 0.037 Tier I
6 NC_000004.12 4 8408853 + ACOX3 NNNTGCCATTGGCATCCAAA NGG 2 0.5625 Tier II
7 NC_000011.10 11 101553514 - TRPC6 NNNTAACATTGGCATCCAAG NGG 2 0.5306 Tier II
8 NC_000005.10 5 141956185 + RNF14 NNNTATCATTGGCATCCAAG NGG 2 0.5306 Tier II
9 NC_000016.10 16 73033777 + ZFHX3 NNNTCCCACTGGCATCCTAG NGG 2 0.4615 Tier II
10 NC_000010.11 10 117341493 + PDZD8 NNNTCCCCTTGGCATCCATG NGG 2 0.2308 Tier II
11 NC_000011.10 11 62499704 - AHNAK NNNTCTCATTGGCATCCCAG NGG 2 0.1769 Tier II
12 NC_000011.10 11 64804932 + MEN1 NNNTCCCATCGGCACCCAAG NGG 2 0.1455 Tier II
13 NC_000016.10 16 48126041 + ABCC12 NNNTCCCATTTGCCTCCAAG NGG 2 0.0824 Tier II
14 NC_000012.12 12 131751819 + SFSWAP NNNTCCCATTGACATCCAAG NGT 2 0.0151 Tier II
15 NC_000004.12 4 89638064 - LOC105377329 NNNGCTCATTGGCATCCAAG NGG 2 0.5804 Tier III
16 NC_000016.10 16 54952892 + LOC105371277 NNNTCCCAGTGACATCCAAG NGG 2 0.5778 Tier III
17 NC_000007.14 7 128218075 - LOC124901744 NNNTCCCATGGGCATCCAAC NGG 2 0.2143 Tier III
18 NC_000012.12 12 52735756 - KRT125P NNNTCCCTTTGGCATCCAGG NGG 2 0.1607 Tier III
19 NC_000006.12 6 169223349 + THBS2-AS1 NNNTCCCTTTGGGATCCAAG NGG 2 0.0584 Tier III
20 NC_000003.12 3 130233369 + COL6A4P2 NNNTTCCATTGGCATCCAAG NTG 2 0.0248 Tier III
21 NC_000001.11 1 147448830 - LINC00624 NNNTCCCAGTGGCATCCAAG NTG 2 0.0241 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 38282404 + Pcdh12 NNNTATCATTGGCATCCAAG NGG 2 0.5306 Tier I
2 NC_000071.6 5 125778835 + Tmem132b NNNTCCCAGTGGCATCCAAC NGG 2 0.2653 Tier I
3 NC_000084.6 18 71370674 + Dcc NNNTCCCATTGGCTTCCAAG NGA 2 0.037 Tier I
4 NC_000078.6 12 102152142 - Slc24a4 NNNTCCCAATAGCATCCAAG NGG 2 0.9286 Tier II
5 NC_000069.6 3 88449932 + Sema4a NNNTCCCATTGGCTTCCAAG NGG 1 0.5333 Tier II
6 NC_000084.6 18 38282404 + Rnf14 NNNTATCATTGGCATCCAAG NGG 2 0.5306 Tier II
7 NC_000085.6 19 21472669 - Gda NNNTCCTATTGGCATCCAGG NGG 2 0.3047 Tier II
8 NC_000083.6 17 29697328 + Ccdc167 NNNTTCCATTGGCATCAAAG NGG 2 0.297 Tier II
9 NC_000083.6 17 29697328 + Cmtr1 NNNTTCCATTGGCATCAAAG NGG 2 0.297 Tier II
10 NC_000069.6 3 144200068 + Lmo4 NNNTCCCATGGGAATCCAAG NGG 2 0.1923 Tier II
11 NC_000068.7 2 165364599 + Zfp663 NNNTCCCAGTGGCATCCAAG NAG 2 0.1605 Tier II
12 NC_000077.6 11 81164630 - Asic2 NNNTCCCATTGTCCTCCAAG NGG 2 0.0824 Tier II
13 NC_000084.6 18 9859736 + Colec12 NNNACCCATTGGCATGCAAG NGG 2 0.0733 Tier II
14 NC_000073.6 7 139862694 + Adgra1 NNNTCCAATTGGCATCCAAG NGA 2 0.0521 Tier II
15 NC_000072.6 6 144062504 + Sox5 NNNTCCCATTGACATCCAAG NTG 2 0.0364 Tier II
16 NC_000082.6 16 11425565 - Snx29 NNNTCCCATTGGCTTCCAAG NGC 2 0.0119 Tier II
17 NC_000070.6 4 3929824 - Plag1 NNNTTCCATTGGCATCCAAG NGT 2 0.0103 Tier II
18 NC_000083.6 17 29697328 + Gm28043 NNNTTCCATTGGCATCAAAG NGG 2 0.297 Tier III
19 NC_000082.6 16 86850089 - Gm32624 NNNTCCAATTGGAATCCAAG NGG 2 0.2885 Tier III
20 NC_000077.6 11 18578316 + Gm12024 NNNTCCCATCTGCATCCAAG NGG 2 0.2051 Tier III
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Other clones with same target sequence:

(none)