Construct: sgRNA BRDN0001145490
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACATGATGAAGTAGTATGT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- RIOK3 (8780)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000018.10 | 18 | 23464596 | + | RIOK3 | NNNATGATGAAGTAGTATGT | NGG | 0 | 1.0 | Tier I |
2 | NC_000012.12 | 12 | 82846041 | - | TMTC2 | NNNATGAGGAAGTAGAATGT | NGG | 2 | 0.6667 | Tier II |
3 | NC_000001.11 | 1 | 153179663 | - | SPRR2G | NNNAGGAAGAAGTAGTATGT | NGG | 2 | 0.512 | Tier II |
4 | NC_000020.11 | 20 | 14414895 | - | MACROD2 | NNNATGAGGAAGAAGTATGT | NGG | 2 | 0.5077 | Tier II |
5 | NC_000017.11 | 17 | 67958046 | + | BPTF | NNNTTGAGGAAGTAGTATGT | NGG | 2 | 0.4667 | Tier II |
6 | NC_000012.12 | 12 | 16212573 | + | SLC15A5 | NNNATGATGGAGAAGTATGT | NGG | 2 | 0.3846 | Tier II |
7 | NC_000014.9 | 14 | 67647853 | + | ARG2 | NNNATCATGAAGTGGTATGT | NGG | 2 | 0.3182 | Tier II |
8 | NC_000014.9 | 14 | 67647853 | + | GPHN | NNNATCATGAAGTGGTATGT | NGG | 2 | 0.3182 | Tier II |
9 | NC_000014.9 | 14 | 67647853 | + | VTI1B | NNNATCATGAAGTGGTATGT | NGG | 2 | 0.3182 | Tier II |
10 | NC_000009.12 | 9 | 15732967 | - | CCDC171 | NNNATGATGAAGTCATATGT | NGG | 2 | 0.2017 | Tier II |
11 | NC_000015.10 | 15 | 76877993 | + | SCAPER | NNNAGGATGAAGGAGTATGT | NGG | 2 | 0.167 | Tier II |
12 | NC_000012.12 | 12 | 32516637 | + | FGD4 | NNNATGATGAAGCAGTCTGT | NGG | 2 | 0.1393 | Tier II |
13 | NC_000018.10 | 18 | 58496403 | - | ALPK2 | NNNATGATGAAGTTTTATGT | NGG | 2 | 0.0762 | Tier II |
14 | NC_000004.12 | 4 | 87232388 | - | KLHL8 | NNNATGATGAAATAGTATGT | NGC | 2 | 0.0207 | Tier II |
15 | NC_000003.12 | 3 | 151311679 | - | GPR87 | NNNATGATGAAGTAGAATGT | NGC | 2 | 0.0202 | Tier II |
16 | NC_000003.12 | 3 | 151311679 | - | MED12L | NNNATGATGAAGTAGAATGT | NGC | 2 | 0.0202 | Tier II |
17 | NC_000001.11 | 1 | 43868172 | + | ST3GAL3 | NNNATGATGAAATAGTATGT | NGT | 2 | 0.0151 | Tier II |
18 | NC_000005.10 | 5 | 39113692 | - | FYB1 | NNNATGATGAAATAGTATGT | NGT | 2 | 0.0151 | Tier II |
19 | NC_000002.12 | 2 | 219909174 | - | LOC105373890 | NNNATGATGAAGTAGAATGT | NGG | 1 | 0.9091 | Tier III |
20 | NC_000013.11 | 13 | 74829063 | + | RIOK3P1 | NNNATCATGAAGTAGTATGT | NGG | 1 | 0.6818 | Tier III |
21 | NC_000001.11 | 1 | 153179663 | - | LOC101928009 | NNNAGGAAGAAGTAGTATGT | NGG | 2 | 0.512 | Tier III |
22 | NC_000006.12 | 6 | 81866463 | + | LINC02542 | NNNATGATAAAGTAGTAAGT | NGG | 2 | 0.4286 | Tier III |
23 | NC_000012.12 | 12 | 16212573 | + | LOC101928362 | NNNATGATGGAGAAGTATGT | NGG | 2 | 0.3846 | Tier III |
24 | NC_000005.10 | 5 | 101727105 | + | LOC105379102 | NNNATGATGAAGTAGTAGTT | NGG | 2 | 0.2222 | Tier III |
25 | NC_000005.10 | 5 | 124112035 | + | LINC01170 | NNNATGATGAAGTAGTAAGT | NAG | 2 | 0.1728 | Tier III |
26 | NC_000006.12 | 6 | 41008796 | + | LOC101929555 | NNNATGATGAAGTGGGATGT | NGG | 2 | 0.1615 | Tier III |
27 | NC_000013.11 | 13 | 97696061 | + | LOC105370324 | NNNATGATGAAGTAGTCTTT | NGG | 2 | 0.1176 | Tier III |
28 | NC_000020.11 | 20 | 33158368 | + | LOC105372714 | NNNATGATGCAGTAGTGTGT | NGG | 2 | 0.0588 | Tier III |
29 | NC_000008.11 | 8 | 29786640 | - | LINC02099 | NNNATGATGAAGAAGTATGT | NGT | 2 | 0.0112 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000084.6 | 18 | 12137317 | + | Riok3 | NNNATGATGAAGTAGTATGT | NGA | 1 | 0.0694 | Tier I |
2 | NC_000072.6 | 6 | 61288413 | + | Ccser1 | NNNATCATGAAGTAGAATGT | NGG | 2 | 0.6198 | Tier II |
3 | NC_000086.7 | X | 38542796 | - | Cul4b | NNNATGCTGAAATAGTATGT | NGG | 2 | 0.4083 | Tier II |
4 | NC_000086.7 | X | 151014876 | + | Gnl3l | NNNATGATGAGGTAGTATGA | NGG | 2 | 0.3656 | Tier II |
5 | NC_000071.6 | 5 | 143488518 | - | Daglb | NNNATGATGATGTAGTATGT | NGG | 1 | 0.3077 | Tier II |
6 | NC_000067.6 | 1 | 93423092 | - | Hdlbp | NNNATGATCAACTAGTATGT | NGG | 2 | 0.2851 | Tier II |
7 | NC_000068.7 | 2 | 139922070 | - | Tasp1 | NNNATGATGAATTAGTAAGT | NGG | 2 | 0.2564 | Tier II |
8 | NC_000067.6 | 1 | 54785500 | - | Ankrd44 | NNNATCATGAAGTAGTATGT | NAG | 2 | 0.1768 | Tier II |
9 | NC_000068.7 | 2 | 106248000 | + | Dcdc5 | NNNATGATGATGTAGGATGT | NGG | 2 | 0.1065 | Tier II |
10 | NC_000081.6 | 15 | 4555739 | + | Plcxd3 | NNNATAATGAAGTAGTATGC | NGG | 2 | 0.0909 | Tier II |
11 | NC_000074.6 | 8 | 81918537 | - | Inpp4b | NNNATGATCAAGTATTATGT | NGG | 2 | 0.0769 | Tier II |
12 | NC_000068.7 | 2 | 130925399 | + | Atrn | NNNAAGATGAAGTAGTTTGT | NGG | 2 | 0.0667 | Tier II |
13 | NC_000077.6 | 11 | 75566127 | - | Slc43a2 | NNNATGATGAAGTAGCATGC | NGG | 2 | 0.0606 | Tier II |
14 | NC_000077.6 | 11 | 31048338 | - | Asb3 | NNNATGATGAAGTAGTGTGC | NGG | 2 | 0.016 | Tier II |
15 | NC_000071.6 | 5 | 146872162 | - | Gm38748 | NNNATGATTAAGAAGTATGT | NGG | 2 | 0.3692 | Tier III |
16 | NC_000068.7 | 2 | 93144239 | - | Gm32627 | NNNATGATGTAGGAGTATGT | NGG | 2 | 0.2302 | Tier III |
17 | NC_000085.6 | 19 | 55134248 | - | Gm31418 | NNNATGATGAGGTAGTATGC | NGG | 2 | 0.0591 | Tier III |