Construct: sgRNA BRDN0001145496

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGTGTTCCGAGCACGCCATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NME6 (10201)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76222

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 48295144 - NME6 NNNGTTCCGAGCACGCCATG NGG 0 1.0 Tier I
2 NC_000023.11 X 47571819 + ARAF NNNGTTCCCAGCACCCCATG NGG 2 0.1469 Tier II
3 NC_000008.11 8 139890309 + TRAPPC9 NNNGTTCCGAGCACCCCATC NGG 2 0.1169 Tier II
4 NC_000009.12 9 92598622 - CENPP NNNGTTCCGAGCTGGCCATG NGG 2 0.0 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 144696944 - Dtd1 NNNGTTCCTAGCATGCCATG NGG 2 0.3911 Tier II
2 NC_000070.6 4 11572614 - Rad54b NNNGTTCTGAGCAAGCCATG NGG 2 0.3062 Tier II
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Other clones with same target sequence:

(none)