Construct: sgRNA BRDN0001145500
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGCAGCGTCCCGGTCAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HUNK (30811)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75941
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
31958866 |
- |
HUNK |
NNNCAGCGTCCCGGTCAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
144397563 |
+ |
CPSF1 |
NNNCAGGGTCCCGGTCAGCA |
NGG |
2 |
0.1882 |
Tier I |
| 3 |
NC_000019.10 |
19 |
50548580 |
- |
LRRC4B |
NNNCAGCATCCCGGTCAACA |
NGC |
2 |
0.0222 |
Tier I |
| 4 |
NC_000022.11 |
22 |
50269495 |
+ |
MAPK11 |
NNNCAGCCTCCCAGTCAACA |
NGG |
2 |
0.568 |
Tier II |
| 5 |
NC_000002.12 |
2 |
239291180 |
- |
HDAC4 |
NNNCAGCGTCCCAGTCAAAA |
NGG |
2 |
0.3956 |
Tier II |
| 6 |
NC_000003.12 |
3 |
8611241 |
- |
LOC107986009 |
NNNCAACGTGCCGGTCAACA |
NGG |
2 |
0.3889 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
37360748 |
+ |
Grhl2 |
NNNCAGCATCCTGGTCAACA |
NGG |
2 |
0.5385 |
Tier I |
| 2 |
NC_000068.7 |
2 |
158625455 |
+ |
Actr5 |
NNNCAACGTCCCGGTCAACC |
NGG |
2 |
0.2273 |
Tier I |
| 3 |
NC_000080.6 |
14 |
19935195 |
+ |
Gng2 |
NNNCAGCATCCGGGTCAACA |
NGG |
2 |
0.4444 |
Tier II |
| 4 |
NC_000070.6 |
4 |
59826986 |
+ |
Snx30 |
NNNCAGCGTCCAGGCCAACA |
NGG |
2 |
0.1948 |
Tier II |
| 5 |
NC_000077.6 |
11 |
4760741 |
- |
Nf2 |
NNNCAGCCTCCCGGTCCACA |
NGG |
2 |
0.1086 |
Tier II |
Other clones with same target sequence:
(none)
