Construct: sgRNA BRDN0001145501

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAACGGCTAGTGTTAAGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PXK (54899)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78017

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 58391216 + PXK NNNACGGCTAGTGTTAAGCT NGG 0 1.0 Tier I
2 NC_000014.9 14 57729762 - SLC35F4 NNNACGGCTAGTGCTAGGCT NGG 2 0.0504 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 49256215 - Enox2 NNNAAGGCTAGTGCTAAGCT NGG 2 0.1633 Tier II
2 NC_000068.7 2 181124608 + Kcnq2 NNNACGGCTAGGGTTATGCT NGG 2 0.0667 Tier II
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Other clones with same target sequence:

(none)