Construct: sgRNA BRDN0001145503

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTGGTTCGGCTCTACGCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LCK (3932)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76412

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 32276736 + LCK NNNGGTTCGGCTCTACGCTG NGG 0 1.0 Tier I
2 NC_000010.11 10 100999577 - LZTS2 NNNGGTTCGGCTCGACGCGG NGG 2 0.0 Tier II
3 NC_000014.9 14 96679341 - LOC105370645 NNNGGTACGTCTCTACGCTG NGG 2 0.7109 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 63379674 - Blk NNNGGTTCGTCTCTACGCTG NGG 1 0.8125 Tier I
2 NC_000072.6 6 122598469 - Apobec1 NNNGGCTCGGCTCTACGCTG NGA 2 0.0631 Tier II
Download CSV

Other clones with same target sequence:

(none)