Construct: sgRNA BRDN0001145504
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGACGGGCCGAGTCATGCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME1 (4830)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75537
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
51161201 |
+ |
NME1 |
NNNACGGGCCGAGTCATGCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
51161201 |
+ |
NME1-NME2 |
NNNACGGGCCGAGTCATGCT |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000023.11 |
X |
10208465 |
- |
CLCN4 |
NNNACAGGCCGAGTCATGTT |
NGG |
2 |
0.4615 |
Tier I |
| 4 |
NC_000017.11 |
17 |
51169978 |
+ |
NME2 |
NNNACAGGCCGAGTGATGCT |
NGG |
2 |
0.05 |
Tier I |
| 5 |
NC_000017.11 |
17 |
51169978 |
+ |
NME1-NME2 |
NNNACAGGCCGAGTGATGCT |
NGG |
2 |
0.05 |
Tier I |
| 6 |
NC_000001.11 |
1 |
65055818 |
- |
JAK1 |
NNNACGGACAGAGTCATGCT |
NGG |
2 |
0.8667 |
Tier II |
| 7 |
NC_000012.12 |
12 |
31009813 |
+ |
TSPAN11 |
NNNACGGGCCGCGTCATCCT |
NGG |
2 |
0.1253 |
Tier II |
| 8 |
NC_000003.12 |
3 |
110562802 |
+ |
LOC105374037 |
NNNACTGGCTGAGTCATGCT |
NGG |
2 |
0.6275 |
Tier III |
| 9 |
NC_000011.10 |
11 |
112273444 |
+ |
LINC02762 |
NNNAGGGGCCAAGTCATGCT |
NGG |
2 |
0.6 |
Tier III |
| 10 |
NC_000001.11 |
1 |
72283779 |
+ |
LOC105378797 |
NNNACGGGGCGGGTCATGCT |
NGG |
2 |
0.4471 |
Tier III |
| 11 |
NC_000009.12 |
9 |
72275691 |
+ |
LOC100289320 |
NNNACAGGCCGAGTGATGCT |
NGG |
2 |
0.05 |
Tier III |
| 12 |
NC_000009.12 |
9 |
98890725 |
+ |
NME2P3 |
NNNACAGGCCGAGTGATGCT |
NGG |
2 |
0.05 |
Tier III |
| 13 |
NC_000012.12 |
12 |
120282527 |
+ |
NME2P1 |
NNNACAGGCCGAGTGATGCT |
NGG |
2 |
0.05 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
7291537 |
+ |
Clcn4 |
NNNACAGGCCGAGTCATGTT |
NGG |
2 |
0.4615 |
Tier I |
| 2 |
NC_000077.6 |
11 |
93951972 |
- |
Nme2 |
NNNACGGGCCGAGTGATGCT |
NGG |
1 |
0.05 |
Tier I |
| 3 |
NC_000083.6 |
17 |
32350427 |
- |
Akap8l |
NNNACGGGCTGAGTCATGCT |
NGG |
1 |
0.9412 |
Tier II |
| 4 |
NC_000080.6 |
14 |
46636472 |
+ |
4933425B07Rik |
NNNAAGGGCTGAGTCATGCT |
NGG |
2 |
0.5378 |
Tier III |
| 5 |
NC_000074.6 |
8 |
70010734 |
- |
Gm7730 |
NNNACGGGCCGAGTGATGCT |
NGG |
1 |
0.05 |
Tier III |
| 6 |
NC_000071.6 |
5 |
149660955 |
- |
Gm5566 |
NNNACTGGCCGAGTGATGCT |
NGG |
2 |
0.0333 |
Tier III |
Other clones with same target sequence:
(none)
