Construct: sgRNA BRDN0001145505

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAGGCTGCTTAGTAAAGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MERTK (10461)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76444

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 111947405 - MERTK NNNGGCTGCTTAGTAAAGTG NGG 0 1.0 Tier I
2 NC_000007.14 7 139179440 - IFT56 NNNGGCTGCTAAGAAAAGTG NGG 2 0.4643 Tier II
3 NC_000011.10 11 92699834 + FAT3 NNNAGGTGCTTAGTAAAGTG NGG 2 0.45 Tier II
4 NC_000016.10 16 72963152 - ZFHX3 NNNGGCTGCTTGGAAAAGTG NGG 2 0.4471 Tier II
5 NC_000003.12 3 56754413 + ARHGEF3 NNNGGCTGCTTGGTAAACTG NGG 2 0.3439 Tier II
6 NC_000016.10 16 87439452 - ZCCHC14 NNNGACTGCTTAGCAAAGTG NGG 2 0.2476 Tier II
7 NC_000005.10 5 142919605 - ARHGAP26 NNNGGCTGCTTTGTGAAGTG NGG 2 0.2167 Tier II
8 NC_000009.12 9 121652191 - DAB2IP NNNGGCTGCTGAGTAAACTG NGG 2 0.1905 Tier II
9 NC_000003.12 3 149150568 - HPS3 NNNGGCTGCTGAGCAAAGTG NGG 2 0.1143 Tier II
10 NC_000014.9 14 103460997 + MARK3 NNNAGCTGCTTAGTAAAGTG NTG 2 0.0351 Tier II
11 NC_000016.10 16 68032292 - DUS2 NNNGGCTGCTTAGTAAGGTG NGA 2 0.0123 Tier II
12 NC_000005.10 5 76687248 - IQGAP2 NNNGGCTGCTTAGTAAAGTG NTC 2 0.0 Tier II
13 NC_000014.9 14 88544684 - PTPN21 NNNGGCTGCTTAGTAAAGTG NTT 2 0.0 Tier II
14 NC_000009.12 9 115291541 + DELEC1 NNNGGTTGCTTAATAAAGTG NGG 2 0.8571 Tier III
15 NC_000009.12 9 94176887 - LINC02603 NNNGGCTGCTGAGTAAAGTC NGG 2 0.1714 Tier III
16 NC_000009.12 9 94176887 - MIRLET7A1HG NNNGGCTGCTGAGTAAAGTC NGG 2 0.1714 Tier III
17 NC_000023.11 X 39403960 - LINC01283 NNNGGCTGCTTAGAAATGTG NGG 2 0.0825 Tier III
18 NC_000014.9 14 81851221 - LOC107984704 NNNGACTGCTTAGTAAAGTG NGC 2 0.0193 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 128738207 - Mertk NNNGGCTGCTTAATAAAGTA NGG 2 0.8654 Tier I
2 NC_000080.6 14 47717355 + Ktn1 NNNGGATGCATAGTAAAGTG NGG 2 0.7959 Tier II
3 NC_000081.6 15 20730287 + Cdh12 NNNGGCTTCTTAGTAAAGTG NGG 1 0.625 Tier II
4 NC_000073.6 7 50345129 - Nell1 NNNGGCTAGTTAGTAAAGTG NGG 2 0.619 Tier II
5 NC_000082.6 16 91575044 + Tmem50b NNNGGCCGCTAAGTAAAGTG NGG 2 0.5156 Tier II
6 NC_000080.6 14 64245911 - Msra NNNGCCTGCTTAGTAAACTG NGG 2 0.3741 Tier II
7 NC_000085.6 19 60814509 + Dennd10 NNNGGTTGCTTTGTAAAGTG NGG 2 0.3095 Tier II
8 NC_000079.6 13 108338164 + Depdc1b NNNGGCTGCTGAGTAAAGTT NGG 2 0.28 Tier II
9 NC_000078.6 12 69915684 - Atl1 NNNGCCTGCTTTGTAAAGTG NGG 2 0.2619 Tier II
10 NC_000069.6 3 20023115 - Hps3 NNNGGCTGCTTTGTAAAATG NGG 2 0.2308 Tier II
11 NC_000076.6 10 25443659 + Epb41l2 NNNGTCTGCTTAGAAAAGTG NGG 2 0.1857 Tier II
12 NC_000071.6 5 100084713 - Tmem150c NNNGGCTTCTTAGTAAAGGG NGG 2 0.1563 Tier II
13 NC_000078.6 12 13234869 + Ddx1 NNNGGCTTCTTAGTAAGGTG NGG 2 0.1103 Tier II
14 NC_000080.6 14 76440366 - Tsc22d1 NNNGGCTGCTTTTTAAAGTG NGG 2 0.1 Tier II
15 NC_000074.6 8 32348447 + Nrg1 NNNGGCTGCTTTGTAGAGTG NGG 2 0.0641 Tier II
16 NC_000068.7 2 55548061 + Kcnj3 NNNGGCTGCTTAGTAAAGTT NGA 2 0.0486 Tier II
17 NC_000076.6 10 13805156 - Aig1 NNNGGCTGCTTAGTCATGTG NGG 2 0.0364 Tier II
18 NC_000077.6 11 76745606 + Gosr1 NNNGGCTGCTTAGTAAAGTG NTT 2 0.0 Tier II
19 NC_000080.6 14 61172520 + Sacs NNNGGCTGCTTAGTATAGTT NGG 2 0.0 Tier II
20 NC_000070.6 4 89376720 + Gm12610 NNNGGCTGCATAGCAAAGTG NGG 2 0.2449 Tier III
21 NC_000077.6 11 63002264 + Cdrt4os1 NNNGGTTGCTTAGTAAAGTG NAG 2 0.2407 Tier III
22 NC_000071.6 5 106102960 + Gm32736 NNNGGCTGCTAAGTCAAGTG NGG 2 0.2045 Tier III
23 NC_000070.6 4 40497480 + 4933428C19Rik NNNGGCTGCTTAGTAAAAAG NGG 2 0.1978 Tier III
24 NC_000071.6 5 100084713 - Tmem150cos NNNGGCTTCTTAGTAAAGGG NGG 2 0.1563 Tier III
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Other clones with same target sequence:

(none)