Construct: sgRNA BRDN0001145509

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCATGGAGATGACCCGCAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CKB (1152)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76589

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 103520542 + CKB NNNTGGAGATGACCCGCAGG NGG 0 1.0 Tier I
2 NC_000017.11 17 75730276 - ITGB4 NNNTGGAGAAGACCAGCAGG NGG 2 0.1905 Tier I
3 NC_000020.11 20 35292123 + FAM83C NNNCGGAGATGACCCGCAGG NAG 2 0.1678 Tier I
4 NC_000011.10 11 17442772 - ABCC8 NNNTGGAGACGACCCGCAGG NAG 2 0.1383 Tier I
5 NC_000010.11 10 87505446 - MINPP1 NNNTGGTGATGAGCCGCAGG NGG 2 0.0597 Tier I
6 NC_000019.10 19 45308482 + CKM NNNTGGAGATGACCCGGAGG NGA 2 0.0041 Tier I
7 NC_000005.10 5 32091003 + PDZD2 NNNTGGAGATCACCCCCAGG NGG 2 0.0 Tier I
8 NC_000021.9 21 26091666 + APP NNNTGGAGATGACCAACAGG NGG 2 0.2222 Tier II
9 NC_000004.12 4 76690458 - SHROOM3 NNNTGGAGATGACCCGCATG NAG 2 0.1728 Tier II
10 NC_000016.10 16 3191303 - OR1F1 NNNTGGAGATGACCCGCCAG NGG 2 0.1361 Tier II
11 NC_000010.11 10 686318 - DIP2C NNNTGGAGAGGCCCCGCAGG NGG 2 0.1316 Tier II
12 NC_000007.14 7 130308227 - CPA4 NNNTGGAGATGACACGCAGG NAG 2 0.0907 Tier II
13 NC_000001.11 1 109187607 - ELAPOR1 NNNTGGAGATGACCCGCAGG NGA 1 0.0694 Tier II
14 NC_000012.12 12 119155381 + SRRM4 NNNTGGAGATGACCTGGAGG NGG 2 0.0039 Tier II
15 NC_000007.14 7 152262329 - KMT2C NNNTGGAGATGACCCTCAGG NGC 2 0.0 Tier II
16 NC_000016.10 16 46843989 + CKBP1 NNNCGGAGATGGCCCGCAGG NGG 2 0.4673 Tier III
17 NC_000013.11 13 27053722 - LOC105370124 NNNTGGAGATGCCCAGCAGG NGG 2 0.0585 Tier III
18 NC_000001.11 1 207194928 - C4BPAP3 NNNTGGAGATGACCAGCAGG NAG 2 0.0576 Tier III
19 NC_000001.11 1 207194928 - LOC107985251 NNNTGGAGATGACCAGCAGG NAG 2 0.0576 Tier III
20 NC_000021.9 21 10465654 + BAGE2 NNNTGGAGATGACCCTCAGG NGC 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 111670235 + Ckb NNNTGGAGATGACTCGCAGG NGG 1 0.7333 Tier I
2 NC_000073.6 7 102484373 + Olfr544 NNNTGAAGATGACCAGCAGG NGG 2 0.2222 Tier I
3 NC_000073.6 7 102494028 + Olfr545 NNNTGAAGATGACCAGCAGG NGG 2 0.2222 Tier I
4 NC_000080.6 14 78502208 - Akap11 NNNTGGAGATGACGAGCAGG NGG 2 0.0 Tier I
5 NC_000073.6 7 46150779 - Abcc8 NNNTGGAGATGACGCGCAGG NAG 2 0.0 Tier I
6 NC_000082.6 16 38483974 - Cd80 NNNTGGAGATGACCCGCAGG NGG 0 1.0 Tier II
7 NC_000067.6 1 65289697 - Pth2r NNNTGGAGGTGACCCGCAGT NGG 2 0.4667 Tier II
8 NC_000084.6 18 21283866 - Garem1 NNNTGGAGCCGACCCGCAGG NGG 2 0.3048 Tier II
9 NC_000071.6 5 121818709 + Sh2b3 NNNTGGTGGTGACCCGCAGG NGG 2 0.2917 Tier II
10 NC_000072.6 6 143914875 + Sox5 NNNTTGAGATGAACCGCAGG NGG 2 0.1154 Tier II
11 NC_000070.6 4 87125001 + Slc24a2 NNNTGGAGGTGAGCCGCAGG NGG 2 0.0909 Tier II
12 NC_000067.6 1 125738352 + Gpr39 NNNTGGTGATGAGCCGCAGG NGG 2 0.0597 Tier II
13 NC_000081.6 15 100260115 + Atf1 NNNTGGAGATGACCTGCAGG NAG 2 0.0173 Tier II
14 NC_000068.7 2 35571444 + Dab2ip NNNTGGAGATCACCCGCAGG NGC 2 0.0095 Tier II
15 NC_000082.6 16 21640119 - Vps8 NNNTGGAGATGACCCTCAGG NGG 1 0.0 Tier II
16 NC_000082.6 16 38483974 - Ck-ps3 NNNTGGAGATGACCCGCAGG NGG 0 1.0 Tier III
17 NC_000070.6 4 122008897 - Gm12892 NNNTGGAGATGACTCGCAGG NGG 1 0.7333 Tier III
18 NC_000083.6 17 53746847 - Ckb-ps2 NNNTGGAGATGACCCGCAGG NGT 1 0.0161 Tier III
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Other clones with same target sequence:

(none)