Construct: sgRNA BRDN0001145510
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGCAGCTGGTACTTTAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRMS (6725)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76649
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
63544338 |
- |
SRMS |
NNNCAGCTGGTACTTTAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
64933218 |
+ |
ANKDD1A |
NNNCAGCTGGGACTTTAGCA |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000016.10 |
16 |
2332615 |
+ |
ABCA3 |
NNNCAGCTTGTACTGTAGCG |
NGG |
2 |
0.0267 |
Tier II |
| 4 |
NC_000013.11 |
13 |
102399907 |
- |
FGF14 |
NNNCAGCTGGTACTTTGGGG |
NGG |
2 |
0.0221 |
Tier II |
| 5 |
NC_000008.11 |
8 |
78528784 |
+ |
PKIA |
NNNCAGCTGGTACTGTAGCG |
NGA |
2 |
0.0035 |
Tier II |
| 6 |
NC_000006.12 |
6 |
10471430 |
+ |
LINC02522 |
NNNAAGCTGGTACTTTAACG |
NGG |
2 |
0.583 |
Tier III |
| 7 |
NC_000010.11 |
10 |
64411461 |
- |
LOC124902439 |
NNNCTGCTGGTACATTAGCG |
NGG |
2 |
0.2251 |
Tier III |
| 8 |
NC_000016.10 |
16 |
2332615 |
+ |
RPS16P7 |
NNNCAGCTTGTACTGTAGCG |
NGG |
2 |
0.0267 |
Tier III |
| 9 |
NC_000008.11 |
8 |
78528784 |
+ |
LOC105375911 |
NNNCAGCTGGTACTGTAGCG |
NGA |
2 |
0.0035 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
37331497 |
- |
Evc |
NNNCAGATGGTACTTTATCG |
NGG |
2 |
0.5 |
Tier II |
| 2 |
NC_000071.6 |
5 |
77302964 |
- |
Noa1 |
NNNCAGCTGGCACTTTAGCT |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000072.6 |
6 |
116125951 |
+ |
Tmcc1 |
NNNCAGCTGGTACCTTAGAG |
NGG |
2 |
0.1224 |
Tier II |
| 4 |
NC_000067.6 |
1 |
152847427 |
- |
Smg7 |
NNNCAGCTGGTACTTTTGTG |
NGG |
2 |
0.0615 |
Tier II |
Other clones with same target sequence:
(none)
