Construct: sgRNA BRDN0001145512
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGGTGACAATATTCGTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAPSS1 (9061)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77611
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
107693907 |
- |
PAPSS1 |
NNNGGTGACAATATTCGTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
243234358 |
+ |
CEP170 |
NNNGGTGACAATATTCTCCA |
NGG |
2 |
0.1071 |
Tier II |
| 3 |
NC_000008.11 |
8 |
76219309 |
- |
LOC102724858 |
NNNGGTGAAAATATTCTTCA |
NGG |
2 |
0.2143 |
Tier III |
| 4 |
NC_000005.10 |
5 |
92430838 |
+ |
LOC105379080 |
NNNGGTGACAATATTCGTAA |
NTG |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000087.7 |
Y |
13378032 |
- |
Gm21454 |
NNNGCTGACAATATTCCTCA |
NGG |
2 |
0.1849 |
Tier I |
| 2 |
NC_000087.7 |
Y |
18090786 |
- |
Ssty1 |
NNNGCTGACAATATTCCTCA |
NGG |
2 |
0.1849 |
Tier I |
| 3 |
NC_000087.7 |
Y |
21096950 |
- |
Gm20910 |
NNNGCTGACAATATTCCTCA |
NGG |
2 |
0.1849 |
Tier I |
| 4 |
NC_000073.6 |
7 |
28562970 |
+ |
Pak4 |
NNNGGTGACAATATCCGTGA |
NGG |
2 |
0.0341 |
Tier I |
| 5 |
NC_000069.6 |
3 |
139685161 |
- |
Stpg2 |
NNNGCTGACAATATTAGTCA |
NGG |
2 |
0.7857 |
Tier II |
| 6 |
NC_000068.7 |
2 |
53065991 |
- |
Fmnl2 |
NNNGGTTACTATATTCGTCA |
NGG |
2 |
0.5042 |
Tier II |
| 7 |
NC_000083.6 |
17 |
85130945 |
+ |
Camkmt |
NNNGGTTACTATATTCGTCA |
NGG |
2 |
0.5042 |
Tier II |
| 8 |
NC_000084.6 |
18 |
62945596 |
- |
Apcdd1 |
NNNGGTGACAATTTTAGTCA |
NGG |
2 |
0.3 |
Tier II |
| 9 |
NC_000082.6 |
16 |
46425061 |
- |
Nectin3 |
NNNGGTGAAAATATTTGTCA |
NGG |
2 |
0.2637 |
Tier II |
| 10 |
NC_000083.6 |
17 |
49046432 |
- |
Lrfn2 |
NNNTGTGAGAATATTCGTCA |
NGG |
2 |
0.2251 |
Tier II |
| 11 |
NC_000084.6 |
18 |
72110280 |
- |
Dcc |
NNNGGTGACAATATTCTTCA |
NGT |
2 |
0.004 |
Tier II |
| 12 |
NC_000078.6 |
12 |
44586619 |
- |
Nrcam |
NNNGGTGACAATATTGGTCA |
NGC |
2 |
0.0034 |
Tier II |
| 13 |
NC_000068.7 |
2 |
128757638 |
+ |
Mertk |
NNNGGTGACAATCGTCGTCA |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
