Construct: sgRNA BRDN0001145518
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTGAAGCTCATGCGTCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAG2 (51422)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77463
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
151781263 |
+ |
PRKAG2 |
NNNGAAGCTCATGCGTCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
36582377 |
+ |
NKX2-8 |
NNNGAAGCTCAGGCGTCCAG |
NGG |
2 |
0.2381 |
Tier I |
| 3 |
NC_000014.9 |
14 |
72312685 |
+ |
RGS6 |
NNNGTAGCTCATGCGTCGAC |
NGG |
2 |
0.1558 |
Tier II |
| 4 |
NC_000019.10 |
19 |
58242042 |
+ |
ZNF544 |
NNNGAAGCTCATGCCTAGAG |
NGG |
2 |
0.1273 |
Tier II |
| 5 |
NC_000004.12 |
4 |
7995727 |
- |
ABLIM2 |
NNNAAAGCTCATGCGTCGAG |
NGA |
2 |
0.0625 |
Tier II |
| 6 |
NC_000016.10 |
16 |
47855861 |
- |
LINC02192 |
NNNGAAGCTCATGGGTCCAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
34349199 |
- |
9430069I07Rik |
NNNGAGGCTCATGCGTGGAG |
NGG |
2 |
0.042 |
Tier I |
| 2 |
NC_000070.6 |
4 |
98512156 |
+ |
Patj |
NNNGAAGCTCATGCAGCGAG |
NGG |
2 |
0.3258 |
Tier II |
| 3 |
NC_000081.6 |
15 |
34349199 |
- |
Matn2 |
NNNGAGGCTCATGCGTGGAG |
NGG |
2 |
0.042 |
Tier II |
Other clones with same target sequence:
(none)
