Construct: sgRNA BRDN0001145518

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTGAAGCTCATGCGTCGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAG2 (51422)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77463

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 151781263 + PRKAG2 NNNGAAGCTCATGCGTCGAG NGG 0 1.0 Tier I
2 NC_000014.9 14 36582377 + NKX2-8 NNNGAAGCTCAGGCGTCCAG NGG 2 0.2381 Tier I
3 NC_000014.9 14 72312685 + RGS6 NNNGTAGCTCATGCGTCGAC NGG 2 0.1558 Tier II
4 NC_000019.10 19 58242042 + ZNF544 NNNGAAGCTCATGCCTAGAG NGG 2 0.1273 Tier II
5 NC_000004.12 4 7995727 - ABLIM2 NNNAAAGCTCATGCGTCGAG NGA 2 0.0625 Tier II
6 NC_000016.10 16 47855861 - LINC02192 NNNGAAGCTCATGGGTCCAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 34349199 - 9430069I07Rik NNNGAGGCTCATGCGTGGAG NGG 2 0.042 Tier I
2 NC_000070.6 4 98512156 + Patj NNNGAAGCTCATGCAGCGAG NGG 2 0.3258 Tier II
3 NC_000081.6 15 34349199 - Matn2 NNNGAGGCTCATGCGTGGAG NGG 2 0.042 Tier II
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Other clones with same target sequence:

(none)