Construct: sgRNA BRDN0001145519
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCGCGCCCTACCTGGATCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SH3BP5 (9467)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76844
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
15332277 |
+ |
SH3BP5 |
NNNCGCCCTACCTGGATCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
48145119 |
- |
PTPRJ |
NNNCTCCCTACCTGGATCCT |
NGG |
2 |
0.21 |
Tier I |
| 3 |
NC_000017.11 |
17 |
74466650 |
- |
CD300A |
NNNGGCCCTACCTGGACCCG |
NGG |
2 |
0.3529 |
Tier II |
| 4 |
NC_000009.12 |
9 |
137211846 |
- |
NDOR1 |
NNNCTCCCTACCTGGATCCC |
NGG |
2 |
0.1286 |
Tier II |
| 5 |
NC_000023.11 |
X |
49166448 |
+ |
MAGIX |
NNNCGCCCAACCTGGATCCG |
NCG |
2 |
0.0995 |
Tier II |
| 6 |
NC_000019.10 |
19 |
16198403 |
- |
AP1M1 |
NNNCGCCCTGCCTGGCTCCG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000017.11 |
17 |
74466650 |
- |
LOC124904056 |
NNNGGCCCTACCTGGACCCG |
NGG |
2 |
0.3529 |
Tier III |
| 8 |
NC_000023.11 |
X |
27287668 |
- |
LOC105373150 |
NNNCGCCCTACCTGGATCCC |
NAG |
2 |
0.1111 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
31435817 |
+ |
Sh3bp5 |
NNNCGTCCTACCTGGATCCG |
NGG |
1 |
0.9286 |
Tier I |
| 2 |
NC_000075.6 |
9 |
107551501 |
+ |
Rassf1 |
NNNCGCCCTGCCTGGATCCT |
NGG |
2 |
0.3889 |
Tier II |
| 3 |
NC_000070.6 |
4 |
44531351 |
+ |
Pax5 |
NNNCACCCTACCTGGATCCC |
NGG |
2 |
0.3714 |
Tier II |
| 4 |
NC_000083.6 |
17 |
66076768 |
+ |
Ankrd12 |
NNNCCCCCTACCTGGCTCCG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000080.6 |
14 |
31435817 |
+ |
Gm35907 |
NNNCGTCCTACCTGGATCCG |
NGG |
1 |
0.9286 |
Tier III |
Other clones with same target sequence:
(none)
