Construct: sgRNA BRDN0001145520

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGTATTGGAATAACTCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHEK1 (1111)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76643

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 125629419 + CHEK1 NNNTATTGGAATAACTCACA NGG 0 1.0 Tier I
2 NC_000021.9 21 40373601 - DSCAM NNNTATTAGAATAACACACA NGG 2 0.9091 Tier II
3 NC_000015.10 15 82214561 - EFL1 NNNCATTGGAGTAACTCACA NGG 2 0.4206 Tier II
4 NC_000023.11 X 38387639 + OTC NNNTATTGGAAGGACTCACA NGG 2 0.3261 Tier II
5 NC_000004.12 4 106237622 - TBCK NNNTCTGGGAATAACTCACA NGG 2 0.2941 Tier II
6 NC_000008.11 8 14269527 + SGCZ NNNTATTTGCATAACTCACA NGG 2 0.2083 Tier II
7 NC_000003.12 3 169110065 - MECOM NNNTATTGGAGTAAATCACA NGG 2 0.1444 Tier II
8 NC_000012.12 12 19294612 - PLEKHA5 NNNTATTGTAATACCTCACA NGG 2 0.1143 Tier II
9 NC_000010.11 10 73161029 + ECD NNNTCTTGGAATAAATCACA NGG 2 0.1111 Tier II
10 NC_000007.14 7 95095525 - PPP1R9A NNNTATTGGAAAAACTCAGA NGG 2 0.1 Tier II
11 NC_000015.10 15 99588832 + MEF2A NNNTATTGGAATTAATCACA NGG 2 0.0667 Tier II
12 NC_000002.12 2 165251877 + SCN2A NNNTGTTGGAATAATTCACA NGG 2 0.048 Tier II
13 NC_000011.10 11 124697148 - SPA17 NNNTATTGCAATAATTCACA NGG 2 0.0359 Tier II
14 NC_000015.10 15 53590404 + WDR72 NNNTATTGGAATAACTTACA NTG 2 0.0182 Tier II
15 NC_000004.12 4 117431259 + LINC01378 NNNTATTATAATAACTCACA NGG 2 0.5333 Tier III
16 NC_000002.12 2 7857661 - LOC105373408 NNNTCATGGAATAACTCACA NGG 2 0.4333 Tier III
17 NC_000006.12 6 106714149 - LOC105377927 NNNTATGGGAATAACTTACA NGG 2 0.2745 Tier III
18 NC_000016.10 16 80346910 + DYNLRB2-AS1 NNNTATTGGAAAAACTCACA NAG 2 0.2074 Tier III
19 NC_000016.10 16 80236643 - DYNLRB2-AS1 NNNTATTGAATTAACTCACA NGG 2 0.1978 Tier III
20 NC_000007.14 7 95095525 - PPP1R9A-AS1 NNNTATTGGAAAAACTCAGA NGG 2 0.1 Tier III
21 NC_000014.9 14 62406667 + LOC105370529 NNNTATAGGAATAATTCACA NGG 2 0.0583 Tier III
22 NC_000002.12 2 177158724 + LOC105373760 NNNTATTGGAATAAGTCAAA NGG 2 0.0214 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 36722646 - Chek1 NNNAATTGGAATAACTCACA NGG 1 0.4762 Tier I
2 NC_000079.6 13 69618025 + Nsun2 NNNTATTAGAACAACTCACA NGG 2 0.9474 Tier II
3 NC_000067.6 1 70516570 + Spag16 NNNTATTAGAATGACTCACA NGG 2 0.6522 Tier II
4 NC_000073.6 7 76924747 + Agbl1 NNNTATTGGAACAACTCACT NGG 2 0.5684 Tier II
5 NC_000082.6 16 45180815 + Atg3 NNNTATTAGAATAACTTACA NGG 2 0.4667 Tier II
6 NC_000071.6 5 84176514 + Epha5 NNNGATTTGAATAACTCACA NGG 2 0.3906 Tier II
7 NC_000086.7 X 84026420 + Dmd NNNTATGGAAATAACTCACA NGG 2 0.3782 Tier II
8 NC_000073.6 7 56434503 + Oca2 NNNTATTGGAATGACTTACA NGG 2 0.3043 Tier II
9 NC_000070.6 4 116434237 + Mast2 NNNTATTGGAATGGCTCACA NGG 2 0.3043 Tier II
10 NC_000077.6 11 76687573 - Trarg1 NNNTATAGGAATAACTCACA NAG 2 0.2269 Tier II
11 NC_000077.6 11 65730787 - Map2k4 NNNTATTGGAATTACTTACA NGG 2 0.14 Tier II
12 NC_000082.6 16 35213660 + Adcy5 NNNTCTTGGAATAACTCACA NGA 2 0.0347 Tier II
13 NC_000075.6 9 100654124 + Stag1 NNNTATTGGAATAACTCACA NTC 2 0.0 Tier II
14 NC_000074.6 8 65049907 + Gm39183 NNNTATTGGAATGACTTACA NGG 2 0.3043 Tier III
15 NC_000078.6 12 68661193 + Gm31307 NNNTATTGCAAGAACTCACA NGG 2 0.2692 Tier III
16 NC_000076.6 10 33992291 - A830082N09Rik NNNTATTGGAATAACTGCCA NGG 2 0.0112 Tier III
17 NC_000084.6 18 64212898 - St8sia3os NNNTATTGAAATAACTCACA NGT 2 0.0104 Tier III
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Other clones with same target sequence:

(none)