Construct: sgRNA BRDN0001145521

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGCACCAGATATTGTCAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA5 (9252)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77372

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 90923139 - RPS6KA5 NNNACCAGATATTGTCAGAG NGG 0 1.0 Tier I
2 NC_000003.12 3 26684419 - LRRC3B NNNACCAGAAATTGTCAGAA NGG 2 0.8036 Tier II
3 NC_000001.11 1 164823645 - PBX1 NNNACCAGATGATGTCAGAG NGG 2 0.52 Tier II
4 NC_000007.14 7 36668000 - AOAH NNNAACAGAAATTGTCAGAG NGG 2 0.4898 Tier II
5 NC_000011.10 11 15971965 - SOX6 NNNACCAGATATCGACAGAG NGG 2 0.4571 Tier II
6 NC_000002.12 2 48446287 + PPP1R21 NNNATCACATATTGTCAGAG NGG 2 0.3916 Tier II
7 NC_000005.10 5 56889908 - MAP3K1 NNNAACAGGTATTGTCAGAG NGG 2 0.381 Tier II
8 NC_000006.12 6 85486401 + NT5E NNNACCAGAAATTCTCAGAG NGG 2 0.3673 Tier II
9 NC_000011.10 11 16254836 + SOX6 NNNAACAGAGATTGTCAGAG NGG 2 0.2857 Tier II
10 NC_000003.12 3 44616154 + ZNF660-ZNF197 NNNAGCAGATATTGTCAGAC NGG 2 0.2571 Tier II
11 NC_000023.11 X 32497112 - DMD NNNACCAGATTTTGTCAGAT NGG 2 0.2154 Tier II
12 NC_000020.11 20 48688359 + PREX1 NNNAACAGATATTTTCAGAG NGG 2 0.1524 Tier II
13 NC_000002.12 2 166443798 - SCN7A NNNACCAGATATTGACAGAG NAG 2 0.1501 Tier II
14 NC_000006.12 6 147922283 - SAMD5 NNNACCAGATATTGTCAGAT NTG 2 0.0273 Tier II
15 NC_000002.12 2 167995833 + STK39 NNNACCAGAAATTGTCAGAG NGC 2 0.019 Tier II
16 NC_000012.12 12 28337999 + CCDC91 NNNACCAGATATTGTCAGGG NTG 2 0.0146 Tier II
17 NC_000008.11 8 107322343 + ANGPT1 NNNACCAGATATTGTCAGAG NAA 2 0.0 Tier II
18 NC_000008.11 8 92481887 - LOC105375639 NNNACCAGGTAATGTCAGAG NGG 2 0.5333 Tier III
19 NC_000008.11 8 56453761 + PENK-AS1 NNNAAAAGATATTGTCAGAG NGG 2 0.5306 Tier III
20 NC_000006.12 6 132997509 + RPL23AP46 NNNACCAGAAATTGACAGAG NGG 2 0.4962 Tier III
21 NC_000019.10 19 15612189 - RPL23AP2 NNNACCAGAAATTGACAGAG NGG 2 0.4962 Tier III
22 NC_000011.10 11 82138511 + MIR4300HG NNNAACAGAAATTGTCAGAG NGG 2 0.4898 Tier III
23 NC_000004.12 4 89603727 + LOC105377329 NNNACCAGCTATTATCAGAG NGG 2 0.4286 Tier III
24 NC_000003.12 3 151809116 + AADACL2-AS1 NNNACCAGATATTCTCAGAA NGG 2 0.4018 Tier III
25 NC_000003.12 3 44616154 + ZKSCAN7-AS1 NNNAGCAGATATTGTCAGAC NGG 2 0.2571 Tier III
26 NC_000012.12 12 14910601 + LOC124902885 NNNACCAGATATTTTCAGAA NGG 2 0.25 Tier III
27 NC_000013.11 13 69314542 - LINC00383 NNNTCCAGATATTTTCAGAG NGG 2 0.1697 Tier III
28 NC_000024.10 Y 25691008 + OFD1P13Y NNNACCAGATATAGTCAGAG NGA 2 0.0481 Tier III
29 NC_000015.10 15 100821822 - LOC105371024 NNNGCCAGATATTGGCAGAG NGG 2 0.0313 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 100597916 - Rps6ka5 NNNTCCAGATATTGTCAGAG NGG 1 0.6364 Tier I
2 NC_000072.6 6 76995534 + Ctnna2 NNNACTAGATGTTGTCAGAG NGG 2 0.6036 Tier II
3 NC_000075.6 9 121417763 + Trak1 NNNGCCAGATACTGTCAGAG NGG 2 0.5921 Tier II
4 NC_000081.6 15 65935701 + Hhla1 NNNACAAGATATTGTCAGTG NGG 2 0.5 Tier II
5 NC_000083.6 17 10689082 - Pacrg NNNATCAGATATTATCAGAG NGG 2 0.4773 Tier II
6 NC_000078.6 12 89361728 + Nrxn3 NNNATCAGATATTGACAGAG NGG 2 0.3684 Tier II
7 NC_000084.6 18 35438609 - Sil1 NNNCCCAGATACTGTCAGAG NGG 2 0.3344 Tier II
8 NC_000077.6 11 58292121 - 4930438A08Rik NNNATCAGAGATTGTCAGAG NGG 2 0.3182 Tier II
9 NC_000071.6 5 86420368 - Tmprss11a NNNACCAGCTATTGTCAGGG NGG 2 0.2143 Tier II
10 NC_000084.6 18 75262562 - Dym NNNACCAGATTTTGTCAGTG NGG 2 0.1657 Tier II
11 NC_000069.6 3 131264813 + Hadh NNNACCAGTTATGGTCAGAG NGG 2 0.1565 Tier II
12 NC_000073.6 7 57644639 - Gabrb3 NNNAGCAGATATTGTCAGAG NTG 2 0.0234 Tier II
13 NC_000083.6 17 32089877 + Pdxk-ps NNNACAAGATATTGTCATAG NGG 2 0.619 Tier III
14 NC_000074.6 8 104097221 - Gm29682 NNNACTAGGTATTGTCAGAG NGG 2 0.619 Tier III
15 NC_000077.6 11 58292121 - Gm39827 NNNATCAGAGATTGTCAGAG NGG 2 0.3182 Tier III
16 NC_000069.6 3 144157732 + Gm34162 NNNCCCAGATGTTGTCAGAG NGG 2 0.2294 Tier III
17 NC_000075.6 9 69808072 + B230323A14Rik NNNACCAGATAGTGTCAGAG NGC 2 0.0111 Tier III
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Other clones with same target sequence:

(none)