Construct: sgRNA BRDN0001145523
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATTGCGGGATATGCCAGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3R3 (8503)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76342
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
46077580 |
- |
PIK3R3 |
NNNTGCGGGATATGCCAGAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
46077580 |
- |
P3R3URF-PIK3R3 |
NNNTGCGGGATATGCCAGAT |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000004.12 |
4 |
78445120 |
+ |
FRAS1 |
NNNTGCAGGATATGCCAGAT |
NGA |
2 |
0.0694 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
116259579 |
+ |
Pik3r3 |
NNNTGCGGGACATGCCAGAT |
NGT |
2 |
0.0108 |
Tier I |
| 2 |
NC_000082.6 |
16 |
59873593 |
- |
Epha6 |
NNNGGCAGGATATGCCAGAT |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000082.6 |
16 |
96168655 |
- |
Lca5l |
NNNTGCAGGATATGCCAGGT |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000080.6 |
14 |
30313943 |
+ |
Cacna1d |
NNNTCCGGGATATGCCAGGT |
NGG |
2 |
0.2946 |
Tier II |
| 5 |
NC_000076.6 |
10 |
67045289 |
- |
Reep3 |
NNNGGCGGGATATGCCAGGT |
NGG |
2 |
0.2344 |
Tier II |
| 6 |
NC_000084.6 |
18 |
49751181 |
- |
Dtwd2 |
NNNTGCTGGATATGCCAGGT |
NGG |
2 |
0.2143 |
Tier II |
| 7 |
NC_000077.6 |
11 |
67587236 |
- |
Gas7 |
NNNTGGGGGAGATGCCAGAT |
NGG |
2 |
0.2 |
Tier II |
| 8 |
NC_000069.6 |
3 |
132841275 |
+ |
Tbck |
NNNTGCGGGAGTTGCCAGAT |
NGG |
2 |
0.1333 |
Tier II |
Other clones with same target sequence:
(none)
