Construct: sgRNA BRDN0001145524

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTGAATGGAAGCACATACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCK2 (5106)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78081

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 24098487 - PCK2 NNNGAATGGAAGCACATACA NGG 0 1.0 Tier I
2 NC_000014.9 14 24098487 - NRL NNNGAATGGAAGCACATACA NGG 0 1.0 Tier II
3 NC_000003.12 3 142702948 + PLS1 NNNGAAAGGAAACACATACA NGG 2 0.8167 Tier II
4 NC_000008.11 8 39112935 - ADAM32 NNNAAATGGAAGCACACACA NGG 2 0.6353 Tier II
5 NC_000018.10 18 25286892 + ZNF521 NNNAAATGAAAGCACATACA NGG 2 0.5786 Tier II
6 NC_000001.11 1 9737840 + CLSTN1 NNNGAATGAAAGCACACACA NGG 2 0.4538 Tier II
7 NC_000002.12 2 178382401 - OSBPL6 NNNGAAGGGAAGAACATACA NGG 2 0.2262 Tier II
8 NC_000007.14 7 31775021 + PDE1C NNNGAATGTAAGCACATGCA NGG 2 0.2133 Tier II
9 NC_000010.11 10 99258722 + HPSE2 NNNGAATGGACCCACATACA NGG 2 0.2118 Tier II
10 NC_000003.12 3 77499456 + ROBO2 NNNCAATGGATGCACATACA NGG 2 0.1629 Tier II
11 NC_000017.11 17 63540014 - KCNH6 NNNGAATGGCAGCACATGCA NGG 2 0.1333 Tier II
12 NC_000007.14 7 3562898 + SDK1 NNNGAAGGGAAGCAAATACA NGG 2 0.1307 Tier II
13 NC_000011.10 11 106880294 + GUCY1A2 NNNGAATGCAAGCCCATACA NGG 2 0.1154 Tier II
14 NC_000014.9 14 103582237 - COA8 NNNGAATGGAACCACATCCA NGG 2 0.1008 Tier II
15 NC_000005.10 5 26913323 + CDH9 NNNTAATGGAAGCACATACA NAG 2 0.0943 Tier II
16 NC_000004.12 4 181874897 - TENM3 NNNGAAGGGAAGCACATAGA NGG 2 0.0735 Tier II
17 NC_000005.10 5 64861732 - CWC27 NNNTAATGGAAGCACATAGA NGG 2 0.0455 Tier II
18 NC_000020.11 20 9606698 + PAK5 NNNGAATGGAAGCAGACACA NGG 2 0.0353 Tier II
19 NC_000006.12 6 29553103 - OR2I1P NNNGAATGGAATCATATACA NGG 2 0.0256 Tier II
20 NC_000023.11 X 33288143 + DMD NNNGAATGGAAGCACATAGA NGA 2 0.0087 Tier II
21 NC_000012.12 12 123676768 + TCTN2 NNNGAATGGAAGCACAGACA NGA 2 0.0082 Tier II
22 NC_000021.9 21 44384430 + TRPM2 NNNGAATGGCAGCACATACA NGC 2 0.0074 Tier II
23 NC_000004.12 4 66905094 - LOC105377262 NNNGAAAGGAAGCAAATACA NGG 2 0.1944 Tier III
24 NC_000001.11 1 55853195 - LOC105378740 NNNGCATGGAAGAACATACA NGG 2 0.1923 Tier III
25 NC_000003.12 3 106877540 + LOC124906264 NNNGAATGGAAGCAAATATA NGG 2 0.1026 Tier III
26 NC_000020.11 20 9606698 + LOC105372523 NNNGAATGGAAGCAGACACA NGG 2 0.0353 Tier III
27 NC_000006.12 6 97514122 - LOC101927314 NNNGAATGGAAGCACATATA NGA 2 0.0321 Tier III
28 NC_000005.10 5 109471383 + LOC105379117 NNNGAATGGAAGCACAGAGA NGG 2 0.0147 Tier III
29 NC_000010.11 10 59062660 + LOC102724768 NNNGAATGGAAGCATATCCA NGG 2 0.0127 Tier III
30 NC_000003.12 3 44111303 + LOC124909489 NNNGAATGGAAGCACATCCA NGC 2 0.0042 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 93274097 + Sned1 NNNGAATGGAGGCACATGCA NGG 2 0.26 Tier I
2 NC_000080.6 14 55543872 - Pck2 NNNGAATGGAAGCACATACA NAA 2 0.0 Tier I
3 NC_000070.6 4 148933250 + Casz1 NNNGAATGGAAGCACTTACA NGA 2 0.0 Tier I
4 NC_000070.6 4 117214162 + Armh1 NNNGAATGGAAGTACTTACA NGG 2 0.0 Tier I
5 NC_000078.6 12 34191507 - Hdac9 NNNGAACAGAAGCACATACA NGG 2 0.6875 Tier II
6 NC_000077.6 11 94671023 - Xylt2 NNNGAAAGGAAGTACATACA NGG 2 0.6125 Tier II
7 NC_000085.6 19 58020368 - Atrnl1 NNNGAAGGGAAACACATACA NGG 2 0.549 Tier II
8 NC_000080.6 14 69359144 - Entpd4 NNNAAATGCAAGCACATACA NGG 2 0.4846 Tier II
9 NC_000080.6 14 69577394 - Entpd4b NNNAAATGCAAGCACATACA NGG 2 0.4846 Tier II
10 NC_000085.6 19 4277104 + Ankrd13d NNNACATGGAAGCACATACA NGG 2 0.45 Tier II
11 NC_000081.6 15 66285363 + Kcnq3 NNNGAAGGGAAGCACACACA NGG 2 0.4152 Tier II
12 NC_000070.6 4 43420793 + Rusc2 NNNGATTGGGAGCACATACA NGG 2 0.3968 Tier II
13 NC_000071.6 5 147491151 + Pan3 NNNGAATGGAAGCACATGCT NGG 2 0.24 Tier II
14 NC_000076.6 10 110294914 + Nav3 NNNGAATGGAAACCCATACA NGG 2 0.2 Tier II
15 NC_000070.6 4 131852137 - Mecr NNNGTATGCAAGCACATACA NGG 2 0.1958 Tier II
16 NC_000082.6 16 75105220 - Robo2 NNNGAATGGAAGTCCATACA NGG 2 0.15 Tier II
17 NC_000069.6 3 64270352 + Vmn2r3 NNNTAATGGAAGCACATGCA NGG 2 0.1455 Tier II
18 NC_000068.7 2 52405270 - Arl5a NNNGAATGTAAGCCCATACA NGG 2 0.1143 Tier II
19 NC_000078.6 12 117677228 + Rapgef5 NNNGAATGGAACCACATCCA NGG 2 0.1008 Tier II
20 NC_000069.6 3 84978708 + Fbxw7 NNNGAATGGAAGAACATACA NAG 2 0.0997 Tier II
21 NC_000086.7 X 162824730 + Txlng NNNGAATGGAAGAACATACA NAG 2 0.0997 Tier II
22 NC_000081.6 15 92575338 + Pdzrn4 NNNCAATGGAAGCACAGACA NGG 2 0.0623 Tier II
23 NC_000077.6 11 33836217 + Kcnip1 NNNGAATGGAAGCAGATACA NGG 1 0.05 Tier II
24 NC_000067.6 1 126251277 + Nckap5 NNNGAATGGAAGCACATACT NTG 2 0.0234 Tier II
25 NC_000068.7 2 120210633 + Pla2g4e NNNGGATGGAAGCACATACA NGC 2 0.016 Tier II
26 NC_000071.6 5 65417960 - Ugdh NNNGAATGGAAGCACATATA NGT 2 0.0074 Tier II
27 NC_000079.6 13 59590154 - Naa35 NNNGAATGGAAGCACAGACA NTG 2 0.0046 Tier II
28 NC_000086.7 X 152733917 + Shroom2 NNNGAATGGAAGCACATACC NGT 2 0.0037 Tier II
29 NC_000068.7 2 55556923 - Kcnj3 NNNGAATGGAAGCACTAACA NGG 2 0.0 Tier II
30 NC_000069.6 3 122083045 + Abca4 NNNGAATGGAAGCACTCACA NGG 2 0.0 Tier II
31 NC_000074.6 8 8679275 - Arglu1 NNNTAATGGAAGCACTTACA NGG 2 0.0 Tier II
32 NC_000085.6 19 58020368 - Gm32980 NNNGAAGGGAAACACATACA NGG 2 0.549 Tier III
33 NC_000081.6 15 66285363 + Gm27242 NNNGAAGGGAAGCACACACA NGG 2 0.4152 Tier III
34 NC_000074.6 8 8093140 - Gm31401 NNNGAATGGAAACAGATACA NGG 2 0.0467 Tier III
35 NC_000082.6 16 49231713 - 1700026J12Rik NNNGAAAGGAAGCACATACA NGT 2 0.0141 Tier III
36 NC_000068.7 2 64382212 - Gm13575 NNNGAATGGAAGCACCTCCA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)