Construct: sgRNA BRDN0001145525
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATATTTATGAGCTAATACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MOK (5891)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75936
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
102263551 |
- |
MOK |
NNNATTTATGAGCTAATACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
42475683 |
- |
ATP8A1 |
NNNATAAATGAGCTAATACG |
NGG |
2 |
0.7583 |
Tier II |
| 3 |
NC_000003.12 |
3 |
45002255 |
+ |
EXOSC7 |
NNNATTTATGAGTAAATACG |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000014.9 |
14 |
88471785 |
+ |
PTPN21 |
NNNATTTATGAGCTAAAAAG |
NGG |
2 |
0.2286 |
Tier II |
| 5 |
NC_000016.10 |
16 |
68444044 |
- |
SMPD3 |
NNNAATTATGAGCTAATAAG |
NGG |
2 |
0.2143 |
Tier II |
| 6 |
NC_000014.9 |
14 |
79305216 |
+ |
NRXN3 |
NNNATTTATGAGCTAATATG |
NGT |
2 |
0.0074 |
Tier II |
| 7 |
NC_000008.11 |
8 |
88569313 |
- |
LOC105375629 |
NNNATTTACGAACTAATACG |
NGG |
2 |
0.8615 |
Tier III |
| 8 |
NC_000008.11 |
8 |
88569313 |
- |
LOC105375630 |
NNNATTTACGAACTAATACG |
NGG |
2 |
0.8615 |
Tier III |
| 9 |
NC_000013.11 |
13 |
27824369 |
- |
PLUT |
NNNATTTATGAGCTAAGACA |
NGG |
2 |
0.1103 |
Tier III |
| 10 |
NC_000002.12 |
2 |
144682368 |
+ |
TEX41 |
NNNATTTATGAGCTAGTGCG |
NGG |
2 |
0.0769 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
110826447 |
- |
Mok |
NNNATTTATGAGCTAATACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000071.6 |
5 |
70792224 |
- |
Gabrg1 |
NNNATTTATGGGCTAATACG |
NAG |
2 |
0.1685 |
Tier II |
| 3 |
NC_000080.6 |
14 |
120790921 |
+ |
Gm41253 |
NNNAGTTATGAGCTAATATG |
NGG |
2 |
0.2954 |
Tier III |
Other clones with same target sequence:
(none)
