Construct: sgRNA BRDN0001145526

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAGCACCCGCTTCTCAACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKCD (5580)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76002

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 53186267 - PRKCD NNNCACCCGCTTCTCAACCA NGG 0 1.0 Tier I
2 NC_000016.10 16 24154784 - PRKCB NNNCACCCGCTTCTCCACCA NAG 2 0.0 Tier I
3 NC_000006.12 6 43043085 + CUL7 NNNCACCTGCTTCTCCACCA NGG 2 0.0 Tier I
4 NC_000008.11 8 1757294 + CLN8 NNNAACCTGCTTCTCAACCA NGG 2 0.7368 Tier II
5 NC_000016.10 16 17215945 - XYLT1 NNNCACCCACTCCTCAACCA NGG 2 0.609 Tier II
6 NC_000012.12 12 132255112 + GALNT9 NNNGAGCCGCTTCTCAACCA NGG 2 0.25 Tier II
7 NC_000003.12 3 141342598 - ZBTB38 NNNCACCCGCTTCTCAACCG NAG 2 0.1983 Tier II
8 NC_000002.12 2 88172538 + THNSL2 NNNCACCCTCTTCTCAACCC NGG 2 0.1212 Tier II
9 NC_000017.11 17 12982502 + ARHGAP44 NNNCACCTGCTTCTCATCCA NGG 2 0.1167 Tier II
10 NC_000017.11 17 66750208 + PRKCA NNNCAGCCGCTTCTGAACCA NGG 2 0.025 Tier II
11 NC_000006.12 6 44290018 - TCTE1 NNNCACCCGCCTCTCAACCA NGC 2 0.0148 Tier II
12 NC_000006.12 6 41741957 + PGC NNNCACCCGCTTCTCCACCA NGA 2 0.0 Tier II
13 NC_000016.10 16 8678516 + ABAT NNNCACCCGCTTCTCCACCA NGC 2 0.0 Tier II
14 NC_000011.10 11 112283557 - LINC02762 NNNCACCTGCTTCTCAATCA NGG 2 0.5625 Tier III
15 NC_000011.10 11 9764586 + SBF2-AS1 NNNCACCCTCCTCTCAACCA NGG 2 0.3556 Tier III
16 NC_000008.11 8 126620224 - LOC105375751 NNNCAGCCCCTTCTCAACCA NGG 2 0.2692 Tier III
17 NC_000008.11 8 65538603 + LINC01299 NNNGACCCGCTTCTCAACCC NGG 2 0.1136 Tier III
18 NC_000017.11 17 77274369 - SEPTIN9-DT NNNCACCCGCTTCTCCACCA NGT 2 0.0 Tier III
19 NC_000006.12 6 43043085 + LOC124901318 NNNCACCTGCTTCTCCACCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 122568234 - Prkcb NNNCACCCGCTTCTCCACCA NGG 1 0.0 Tier I
2 NC_000080.6 14 30602107 + Prkcd NNNCACCCGCTTCTCCACCA NGG 1 0.0 Tier I
3 NC_000077.6 11 107983959 + Prkca NNNCACGCGCTTCTCCACCA NGG 2 0.0 Tier I
4 NC_000084.6 18 25727699 - Celf4 NNNCACCCCCATCTCAACCA NGG 2 0.4038 Tier II
5 NC_000076.6 10 53650046 - Fam184a NNNCACCCCCTTCTCAACCT NGG 2 0.3231 Tier II
6 NC_000068.7 2 175086117 - Gm14393 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
7 NC_000068.7 2 175086117 - LOC115489542 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
8 NC_000068.7 2 175282907 - Gm14440 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
9 NC_000068.7 2 175380063 - Zfp968 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
10 NC_000068.7 2 175477615 - Gm4724 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
11 NC_000068.7 2 175477615 - Zfp965 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
12 NC_000068.7 2 175696098 + Gm4724 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
13 NC_000068.7 2 175793256 + Gm14288 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
14 NC_000068.7 2 175923500 + Zfp966 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
15 NC_000068.7 2 176234915 - 2210418O10Rik NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
16 NC_000068.7 2 176528605 - Zfp967 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
17 NC_000068.7 2 176678152 - Zfp973 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
18 NC_000068.7 2 176919464 + Gm14296 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
19 NC_000068.7 2 177472902 - Zfp970 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
20 NC_000068.7 2 177767640 - Gm14322 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
21 NC_000068.7 2 177835025 + Gm14325 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
22 NC_000068.7 2 177903901 - Zfp972 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
23 NC_000068.7 2 178070358 + Zfp931 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier II
24 NC_000076.6 10 20988497 - Ahi1 NNNCACACGCTTCTCATCCA NGG 2 0.1 Tier II
25 NC_000067.6 1 59599842 + Gm973 NNNCACCTGCTTCTCAACCA NGA 2 0.0608 Tier II
26 NC_000073.6 7 113817461 + Spon1 NNNCACCCGCTTCTCAGCCA NAG 2 0.0458 Tier II
27 NC_000069.6 3 129965266 + Mcub NNNCACCAGCTTCTCTACCA NGG 2 0.0 Tier II
28 NC_000078.6 12 29999307 - Pxdn NNNCACCCGCTTCTCCATCA NGG 2 0.0 Tier II
29 NC_000068.7 2 176431438 - Zfp968-ps NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier III
30 NC_000068.7 2 176855933 + Gm14405 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier III
31 NC_000068.7 2 176855933 + Gm14408 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier III
32 NC_000068.7 2 176919464 + Gm14408 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier III
33 NC_000068.7 2 177138276 - LOC115489545 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier III
34 NC_000068.7 2 177389958 + Gm14418 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier III
35 NC_000068.7 2 177389958 + Gm14412 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier III
36 NC_000068.7 2 177903901 - Gm14327 NNNCACCCCCTTCTCAACCA NAG 2 0.1396 Tier III
37 NC_000068.7 2 177267540 + Gm14412 NNNCACCCTCTTCTCAACCA NAG 2 0.1383 Tier III
38 NC_000068.7 2 177267540 + LOC115489547 NNNCACCCTCTTCTCAACCA NAG 2 0.1383 Tier III
39 NC_000068.7 2 177572224 + Gm14406 NNNCACCCTCTTCTCAACCA NAG 2 0.1383 Tier III
40 NC_000082.6 16 20253541 + Gm49566 NNNCACCCTCTTCTCATCCA NGG 2 0.0711 Tier III
41 NC_000071.6 5 113472309 - Gm30812 NNNCACCCACTTCTCAACCA NGT 2 0.0104 Tier III
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Other clones with same target sequence:

(none)