Construct: sgRNA BRDN0001145528
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGGGTATAACCAACCCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PCK1 (5105)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76288
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
57563584 |
+ |
PCK1 |
NNNGGGTATAACCAACCCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
111500049 |
- |
STARD4 |
NNNGGATATAACCATCCCTG |
NGG |
2 |
0.2 |
Tier I |
| 3 |
NC_000012.12 |
12 |
81524198 |
- |
PPFIA2 |
NNNGGGTAGAACCAACCCTA |
NGG |
2 |
0.5804 |
Tier II |
| 4 |
NC_000001.11 |
1 |
158987274 |
+ |
PYHIN1 |
NNNGGGTATAACCAACCCAG |
NGG |
1 |
0.2857 |
Tier II |
| 5 |
NC_000019.10 |
19 |
2243246 |
- |
SF3A2 |
NNNGGGTCTAACCAGCCCTG |
NGG |
2 |
0.2786 |
Tier II |
| 6 |
NC_000005.10 |
5 |
90751362 |
- |
ADGRV1 |
NNNGGTTATACCCAACCCTG |
NGG |
2 |
0.2667 |
Tier II |
| 7 |
NC_000006.12 |
6 |
37309777 |
+ |
TBC1D22B |
NNNGGGTATATCCATCCCTG |
NGG |
2 |
0.0615 |
Tier II |
| 8 |
NC_000007.14 |
7 |
106581122 |
+ |
CTB-30L5.1 |
NNNGGGTATCACCAACCATG |
NGG |
2 |
0.1795 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
33203758 |
- |
Stard4 |
NNNGGCTATAACCATCCCTG |
NGG |
2 |
0.1364 |
Tier I |
| 2 |
NC_000067.6 |
1 |
36169290 |
+ |
Uggt1 |
NNNGGGAATAACCTACCCTG |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000079.6 |
13 |
103751554 |
+ |
Srek1 |
NNNGGGTATAAACAACCTTG |
NGG |
2 |
0.4592 |
Tier II |
| 4 |
NC_000070.6 |
4 |
6739232 |
+ |
Tox |
NNNGGCTATACCCAACCCTG |
NGG |
2 |
0.2727 |
Tier II |
| 5 |
NC_000067.6 |
1 |
151719567 |
- |
Niban1 |
NNNGGGTAAAACCAACCCTG |
NAG |
2 |
0.2407 |
Tier II |
| 6 |
NC_000068.7 |
2 |
96932268 |
+ |
Lrrc4c |
NNNGGGTAAAACCAACCCTG |
NAG |
2 |
0.2407 |
Tier II |
| 7 |
NC_000080.6 |
14 |
63382771 |
- |
Blk |
NNNGGGTATCACCAACTCTG |
NGG |
2 |
0.1556 |
Tier II |
| 8 |
NC_000068.7 |
2 |
11500524 |
- |
Pfkfb3 |
NNNGGGTAAAACCAAGCCTG |
NGG |
2 |
0.1429 |
Tier II |
| 9 |
NC_000073.6 |
7 |
81872010 |
- |
Tm6sf1 |
NNNGGGTTTAACCAACCCTG |
NAG |
2 |
0.1111 |
Tier II |
| 10 |
NC_000070.6 |
4 |
137022431 |
- |
Zbtb40 |
NNNGGGTATAAGCCACCCTG |
NGG |
2 |
0.0952 |
Tier II |
| 11 |
NC_000071.6 |
5 |
110986491 |
+ |
Ttc28 |
NNNGGGTTTAACCATCCCTG |
NGG |
2 |
0.0857 |
Tier II |
| 12 |
NC_000070.6 |
4 |
98542291 |
- |
Patj |
NNNGGGTATTACCAACCCTG |
NGC |
2 |
0.0196 |
Tier II |
| 13 |
NC_000078.6 |
12 |
90233846 |
+ |
Nrxn3 |
NNNGGGTATAACCAACCCTG |
NTC |
2 |
0.0 |
Tier II |
| 14 |
NC_000086.7 |
X |
84014925 |
- |
Dmd |
NNNGGGTATAACCAACCCTG |
NTT |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
