Construct: sgRNA BRDN0001145538
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGCCAGAAGAATGGTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PI4KA (5297)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76817
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
20765216 |
- |
PI4KA |
NNNGCCAGAAGAATGGTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
144012729 |
+ |
SPATC1 |
NNNGCCAGAACAATGGTAAG |
NGG |
2 |
0.1837 |
Tier I |
| 3 |
NC_000001.11 |
1 |
48823068 |
- |
AGBL4 |
NNNGCCAGAAGAGTGGTACA |
NGG |
2 |
0.6114 |
Tier II |
| 4 |
NC_000001.11 |
1 |
47773524 |
- |
TRABD2B |
NNNGCCACAAGAATAGTACG |
NGG |
2 |
0.5792 |
Tier II |
| 5 |
NC_000010.11 |
10 |
59829779 |
+ |
CCDC6 |
NNNGACAGATGAATGGTACG |
NGG |
2 |
0.5042 |
Tier II |
| 6 |
NC_000005.10 |
5 |
154277838 |
- |
GALNT10 |
NNNGCCAGAAGAATGGTTCT |
NGG |
2 |
0.35 |
Tier II |
| 7 |
NC_000008.11 |
8 |
140097040 |
+ |
TRAPPC9 |
NNNGCCAGAACAATGGCACG |
NGG |
2 |
0.3025 |
Tier II |
| 8 |
NC_000008.11 |
8 |
144012729 |
+ |
PARP10 |
NNNGCCAGAACAATGGTAAG |
NGG |
2 |
0.1837 |
Tier II |
| 9 |
NC_000014.9 |
14 |
88653870 |
+ |
EML5 |
NNNGCCAGAAGAATGGTACC |
NGC |
2 |
0.0095 |
Tier II |
| 10 |
NC_000019.10 |
19 |
49175521 |
+ |
TRPM4 |
NNNGCCAGAAGAATTTTACG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000008.11 |
8 |
140097040 |
+ |
PEG13 |
NNNGCCAGAACAATGGCACG |
NGG |
2 |
0.3025 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
88601075 |
+ |
Ston1 |
NNNCCCAGAGGAATGGTACG |
NGG |
2 |
0.2941 |
Tier II |
| 2 |
NC_000071.6 |
5 |
142000754 |
- |
Sdk1 |
NNNGCCAGAGGAATGGTACC |
NGG |
2 |
0.2381 |
Tier II |
| 3 |
NC_000079.6 |
13 |
75731206 |
+ |
Ell2 |
NNNGCCAGAAAACTGGTACG |
NGG |
2 |
0.2105 |
Tier II |
| 4 |
NC_000078.6 |
12 |
21658269 |
+ |
Gm4036 |
NNNGCCAGGGGAATGGTACG |
NGG |
2 |
0.3704 |
Tier III |
| 5 |
NC_000082.6 |
16 |
50619319 |
+ |
4930542D17Rik |
NNNGGCAGAAGAATGGTAAG |
NGG |
2 |
0.2571 |
Tier III |
Other clones with same target sequence:
(none)
