Construct: sgRNA BRDN0001145540
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGGGAGACTCATGAGATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK5 (1020)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77542
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
151057113 |
- |
CDK5 |
NNNGGAGACTCATGAGATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
33231645 |
+ |
ASIC2 |
NNNGGAGACCCATCAGATCG |
NGG |
2 |
0.2286 |
Tier II |
| 3 |
NC_000014.9 |
14 |
102337999 |
+ |
ZNF839 |
NNNGGAGACTCAGGAGATGG |
NGG |
2 |
0.0326 |
Tier II |
| 4 |
NC_000015.10 |
15 |
29001565 |
+ |
APBA2 |
NNNGGAGACTCATGAGTTGG |
NGG |
2 |
0.0167 |
Tier II |
| 5 |
NC_000009.12 |
9 |
72161252 |
- |
GDA |
NNNGGAGACTCATGAGATCA |
NGT |
2 |
0.0151 |
Tier II |
| 6 |
NC_000008.11 |
8 |
122769359 |
+ |
CDK5P1 |
NNNGGAGACTCATGAGATTG |
NGG |
1 |
0.4615 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
24422839 |
- |
Cdk5 |
NNNGGAAACTCATGAGATTG |
NGG |
2 |
0.4615 |
Tier I |
| 2 |
NC_000085.6 |
19 |
6171591 |
- |
Batf2 |
NNNGGAGACTCATGAGAGCA |
NGG |
2 |
0.3125 |
Tier I |
| 3 |
NC_000086.7 |
X |
103754644 |
- |
Slc16a2 |
NNNGGAGAATCATGAAATCG |
NGG |
2 |
0.8571 |
Tier II |
| 4 |
NC_000073.6 |
7 |
125986362 |
- |
Gsg1l |
NNNGGAGGCTCATGAGATCA |
NGG |
2 |
0.6875 |
Tier II |
| 5 |
NC_000086.7 |
X |
162924278 |
- |
Ctps2 |
NNNGGAGAATCATGAGATTG |
NGG |
2 |
0.3956 |
Tier II |
| 6 |
NC_000075.6 |
9 |
80098217 |
+ |
Senp6 |
NNNGGAGACTAATGAGATCC |
NGG |
2 |
0.3214 |
Tier II |
| 7 |
NC_000079.6 |
13 |
104478113 |
+ |
Adamts6 |
NNNGGAGACTCTTGAGATCA |
NGG |
2 |
0.3125 |
Tier II |
| 8 |
NC_000075.6 |
9 |
106289864 |
+ |
Poc1a |
NNNGGAGACCCATGAGATAG |
NGG |
2 |
0.2286 |
Tier II |
| 9 |
NC_000068.7 |
2 |
20642140 |
- |
Etl4 |
NNNGGAGACTCATGAGACTG |
NGG |
2 |
0.1978 |
Tier II |
| 10 |
NC_000085.6 |
19 |
47976343 |
- |
Cfap58 |
NNNTGAGACTCATGAGATTG |
NGG |
2 |
0.1678 |
Tier II |
| 11 |
NC_000067.6 |
1 |
132489359 |
+ |
Rbbp5 |
NNNGGAGACTCATGAGTTCA |
NGG |
2 |
0.125 |
Tier II |
| 12 |
NC_000078.6 |
12 |
105754539 |
+ |
Ak7 |
NNNGGAGAATCATGAGTTCG |
NGG |
2 |
0.1143 |
Tier II |
| 13 |
NC_000073.6 |
7 |
97445784 |
+ |
Kctd14 |
NNNGGAGACTCAAGAGTTCG |
NGG |
2 |
0.0923 |
Tier II |
Other clones with same target sequence:
(none)
