Construct: sgRNA BRDN0001145543

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGTGATGGAACAGACACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FPGT-TNNI3K (100526835)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75902

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 74367920 - TNNI3K NNNTGATGGAACAGACACAT NGG 0 1.0 Tier I
2 NC_000001.11 1 74367920 - FPGT-TNNI3K NNNTGATGGAACAGACACAT NGG 0 1.0 Tier I
3 NC_000011.10 11 216703 - SIRT3 NNNTGATGGACCAGACAAAT NGG 2 0.2154 Tier I
4 NC_000003.12 3 100539253 + TMEM45A NNNTGAAAGAACAGACACAT NGG 2 0.875 Tier II
5 NC_000021.9 21 38290406 + KCNJ15 NNNTAATGGAACAGAAACAT NGG 2 0.8667 Tier II
6 NC_000007.14 7 31070986 + ADCYAP1R1 NNNTCATGGAACAGAAACAT NGG 2 0.7857 Tier II
7 NC_000006.12 6 138218553 - ARFGEF3 NNNGGATGGAACAGAAACAT NGG 2 0.625 Tier II
8 NC_000003.12 3 38873697 - SCN11A NNNTGATGGAACAGAAACTT NGG 2 0.5385 Tier II
9 NC_000005.10 5 83497098 + VCAN NNNTGATGGAACACACACAT NGG 1 0.4286 Tier II
10 NC_000011.10 11 126599571 - KIRREL3 NNNTGATGGAAAAGACAAAT NGG 2 0.3846 Tier II
11 NC_000001.11 1 3632985 + WRAP73 NNNTGAGGAAACAGACACAT NGG 2 0.3782 Tier II
12 NC_000019.10 19 39173485 - PAK4 NNNCGATGGGACAGACACAT NGG 2 0.3595 Tier II
13 NC_000001.11 1 49690841 - AGBL4 NNNTGATGGAAGAAACACAT NGG 2 0.3333 Tier II
14 NC_000010.11 10 21623039 + MLLT10 NNNGGATGGAAGAGACACAT NGG 2 0.2778 Tier II
15 NC_000001.11 1 7369538 + CAMTA1 NNNTGAGGGAAGAGACACAT NGG 2 0.2614 Tier II
16 NC_000016.10 16 10684135 + TEKT5 NNNTGATGGTACAGACACAT NAG 2 0.2288 Tier II
17 NC_000018.10 18 33408762 + CCDC178 NNNTTATGGAAAAGACACAT NGG 2 0.2143 Tier II
18 NC_000002.12 2 206448809 - ADAM23 NNNAGATGGAAGAGACACAT NGG 2 0.2116 Tier II
19 NC_000008.11 8 135588311 + KHDRBS3 NNNAGATGGAAGAGACACAT NGG 2 0.2116 Tier II
20 NC_000009.12 9 17593108 - SH3GL2 NNNAGATGGAAGAGACACAT NGG 2 0.2116 Tier II
21 NC_000020.11 20 38830195 - PPP1R16B NNNGGATGGCACAGACACAT NGG 2 0.2083 Tier II
22 NC_000001.11 1 246181681 + SMYD3 NNNTTATGAAACAGACACAT NGG 2 0.1929 Tier II
23 NC_000012.12 12 120306992 + SIRT4 NNNTGAGGGAACAGATACAT NGG 2 0.181 Tier II
24 NC_000002.12 2 100058511 + AFF3 NNNTGATGAAACAGCCACAT NGG 2 0.1753 Tier II
25 NC_000003.12 3 184181067 - AP2M1 NNNTGATGGAACAGATAAAT NGG 2 0.1657 Tier II
26 NC_000009.12 9 113986050 + ZNF618 NNNTGATGGGACAGACACAT NAG 2 0.144 Tier II
27 NC_000018.10 18 46656779 + LOXHD1 NNNATATGGAACAGACACAT NGG 2 0.1429 Tier II
28 NC_000010.11 10 125626652 + TEX36 NNNTGATGGAGCAGACACAG NGG 2 0.1147 Tier II
29 NC_000001.11 1 160427734 - VANGL2 NNNTGATGGGACAGACACAG NGG 2 0.098 Tier II
30 NC_000009.12 9 132571752 + CFAP77 NNNTGATGTAACAGACACAG NGG 2 0.0941 Tier II
31 NC_000001.11 1 10014342 - RBP7 NNNTGATGGAAGAGACGCAT NGG 2 0.0784 Tier II
32 NC_000008.11 8 102853136 + AZIN1 NNNTGATGGAACACACAGAT NGG 2 0.0571 Tier II
33 NC_000023.11 X 49213218 + CACNA1F NNNTGATGGATCAGAGACAT NGG 2 0.0473 Tier II
34 NC_000009.12 9 15891504 + CCDC171 NNNTTATGGAACAGACACAC NGG 2 0.0273 Tier II
35 NC_000004.12 4 181700048 - TENM3 NNNTGATGGAACAGCCACAC NGG 2 0.0248 Tier II
36 NC_000005.10 5 128382395 - FBN2 NNNTTATGGAACAGACACAT NGA 2 0.0208 Tier II
37 NC_000011.10 11 22662045 - GAS2 NNNTTATGGAACAGACACAT NGA 2 0.0208 Tier II
38 NC_000021.9 21 6520636 + LOC102724652 NNNTGATGGAACAGACCCAC NGG 2 0.016 Tier II
39 NC_000010.11 10 103712454 - SH3PXD2A NNNTGATGGAACAGACACAG NGA 2 0.0123 Tier II
40 NC_000012.12 12 127464854 - LOC105370066 NNNTGATGGTACAGACACAA NGG 2 0.4963 Tier III
41 NC_000004.12 4 12969702 + LOC105374493 NNNTGATGGAAGAGAAACAT NGG 2 0.4444 Tier III
42 NC_000011.10 11 126599571 - KIRREL3-AS1 NNNTGATGGAAAAGACAAAT NGG 2 0.3846 Tier III
43 NC_000004.12 4 139210371 + LOC105379412 NNNTGAAGGAACACACACAT NGG 2 0.375 Tier III
44 NC_000001.11 1 7369538 + CAMTA1-IT1 NNNTGAGGGAAGAGACACAT NGG 2 0.2614 Tier III
45 NC_000018.10 18 47326377 + MIR4527HG NNNTGGTGGAACTGACACAT NGG 2 0.2143 Tier III
46 NC_000001.11 1 32876048 + LOC105378631 NNNTGATGGATCAGACATAT NGG 2 0.1978 Tier III
47 NC_000012.12 12 93019239 - LOC643339 NNNTGATGGAACTGAGACAT NGG 2 0.0462 Tier III
48 NC_000012.12 12 93019239 - LINC02413 NNNTGATGGAACTGAGACAT NGG 2 0.0462 Tier III
49 NC_000013.11 13 36709354 - LOC102723490 NNNTGATGCAACAGACACAT NGA 2 0.0374 Tier III
50 NC_000007.14 7 94041443 - LOC130890646 NNNTGATGGAACAAACACAT NTG 2 0.0292 Tier III
51 NC_000021.9 21 6520636 + LOC102724701 NNNTGATGGAACAGACCCAC NGG 2 0.016 Tier III
52 NC_000021.9 21 43128952 + LOC101928369 NNNTGATGGAACAGACCCAC NGG 2 0.016 Tier III
53 NC_000017.11 17 79021385 - C1QTNF1-AS1 NNNTGGTGGAACAGACACAT NGT 2 0.0115 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 154941077 + Tnni3k NNNGGATGGGACAGACACAT NGG 2 0.3472 Tier I
2 NC_000070.6 4 102988585 + Tctex1d1 NNNTCATGGCACAGACACAT NGG 2 0.2619 Tier I
3 NC_000082.6 16 97794629 + Prdm15 NNNTGATGGAGCAGTCACAT NGG 2 0.13 Tier I
4 NC_000071.6 5 127549835 + Tmem132c NNNTGATGATACAGACACAT NGG 2 0.5672 Tier II
5 NC_000074.6 8 95339599 + Usb1 NNNCGATGGAAAAGACACAT NGG 2 0.4622 Tier II
6 NC_000072.6 6 53301883 + Creb5 NNNTGATTGAACAGACACTT NGG 2 0.3365 Tier II
7 NC_000083.6 17 51102919 + Tbc1d5 NNNTGCTGAAACAGACACAT NGG 2 0.2922 Tier II
8 NC_000081.6 15 26860009 + Fbxl7 NNNTGCTGGGACAGACACAT NGG 2 0.2525 Tier II
9 NC_000067.6 1 184790829 + Mtarc1 NNNTGATGGAAGAGACACTT NGG 2 0.2393 Tier II
10 NC_000086.7 X 168517362 - Frmpd4 NNNTGGTGGATCAGACACAT NGG 2 0.2198 Tier II
11 NC_000085.6 19 47827204 + Cfap43 NNNTTATGAAACAGACACAT NGG 2 0.1929 Tier II
12 NC_000075.6 9 63286175 + Map2k5 NNNTGATGAAACAGACACAT NAG 2 0.1667 Tier II
13 NC_000077.6 11 119719259 + Rptor NNNTGAAGGAACAGACACAG NGG 2 0.1544 Tier II
14 NC_000067.6 1 188458042 + Ush2a NNNTGATGGAACAATCACAT NGG 2 0.15 Tier II
15 NC_000070.6 4 74388554 - Kdm4c NNNTGATGGAACAAACACAG NGG 2 0.1324 Tier II
16 NC_000078.6 12 29661819 - Myt1l NNNTGATGGAAAAGAGACAT NGG 2 0.1099 Tier II
17 NC_000070.6 4 149004592 - Pex14 NNNTTATGGCACAGACACAT NGG 2 0.1 Tier II
18 NC_000071.6 5 134121245 + Castor2 NNNTGATGGGACAGACACAG NGG 2 0.098 Tier II
19 NC_000080.6 14 27714731 - Erc2 NNNTGATGGCACAGCCACAT NGG 2 0.0909 Tier II
20 NC_000068.7 2 19253038 - Armc3 NNNTGATGGAAGAGTCACAT NGG 2 0.0889 Tier II
21 NC_000083.6 17 71304202 + Emilin2 NNNAGATGGAACAGACACAG NGG 2 0.084 Tier II
22 NC_000069.6 3 69393370 + Ppm1l NNNTGATGGTACAGACACAC NGG 2 0.0802 Tier II
23 NC_000077.6 11 53032807 + Fstl4 NNNTGATGGATCAGACACCT NGG 2 0.0637 Tier II
24 NC_000069.6 3 158373052 + Lrrc7 NNNAGATGGAACAGACAGAT NGG 2 0.0635 Tier II
25 NC_000069.6 3 122247986 - Gclm NNNTGAGGGAACAGACACAC NGG 2 0.0535 Tier II
26 NC_000069.6 3 154052279 + Slc44a5 NNNTGATGGAACAGACAGGT NGG 2 0.05 Tier II
27 NC_000074.6 8 44957072 - Fat1 NNNTGATGGAACAGCCACAG NGG 2 0.0481 Tier II
28 NC_000080.6 14 72861591 + Gm4606 NNNTGATGGAAAAGACACAT NTG 2 0.0278 Tier II
29 NC_000072.6 6 9204400 + Nxph1 NNNTGATTGAACAGACACAT NTG 2 0.0244 Tier II
30 NC_000080.6 14 46963279 - Samd4 NNNTGATGGAACAGAAACAT NGT 2 0.0161 Tier II
31 NC_000070.6 4 102988585 + Gm12709 NNNTCATGGCACAGACACAT NGG 2 0.2619 Tier III
32 NC_000086.7 X 168517362 - Gm7209 NNNTGGTGGATCAGACACAT NGG 2 0.2198 Tier III
33 NC_000070.6 4 149004592 - Gm42351 NNNTTATGGCACAGACACAT NGG 2 0.1 Tier III
34 NC_000068.7 2 128525050 + Gm39928 NNNTGATGGAAAAGACAGAT NGG 2 0.0952 Tier III
35 NC_000080.6 14 27714731 - Gm18398 NNNTGATGGCACAGCCACAT NGG 2 0.0909 Tier III
36 NC_000076.6 10 18789073 + Gm32926 NNNTGATGGATCAGAGACAT NGG 2 0.0473 Tier III
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Other clones with same target sequence:

(none)