Construct: sgRNA BRDN0001145543
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGTGATGGAACAGACACAT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- FPGT-TNNI3K (100526835)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000001.11 | 1 | 74367920 | - | TNNI3K | NNNTGATGGAACAGACACAT | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 74367920 | - | FPGT-TNNI3K | NNNTGATGGAACAGACACAT | NGG | 0 | 1.0 | Tier I |
3 | NC_000011.10 | 11 | 216703 | - | SIRT3 | NNNTGATGGACCAGACAAAT | NGG | 2 | 0.2154 | Tier I |
4 | NC_000003.12 | 3 | 100539253 | + | TMEM45A | NNNTGAAAGAACAGACACAT | NGG | 2 | 0.875 | Tier II |
5 | NC_000021.9 | 21 | 38290406 | + | KCNJ15 | NNNTAATGGAACAGAAACAT | NGG | 2 | 0.8667 | Tier II |
6 | NC_000007.14 | 7 | 31070986 | + | ADCYAP1R1 | NNNTCATGGAACAGAAACAT | NGG | 2 | 0.7857 | Tier II |
7 | NC_000006.12 | 6 | 138218553 | - | ARFGEF3 | NNNGGATGGAACAGAAACAT | NGG | 2 | 0.625 | Tier II |
8 | NC_000003.12 | 3 | 38873697 | - | SCN11A | NNNTGATGGAACAGAAACTT | NGG | 2 | 0.5385 | Tier II |
9 | NC_000005.10 | 5 | 83497098 | + | VCAN | NNNTGATGGAACACACACAT | NGG | 1 | 0.4286 | Tier II |
10 | NC_000011.10 | 11 | 126599571 | - | KIRREL3 | NNNTGATGGAAAAGACAAAT | NGG | 2 | 0.3846 | Tier II |
11 | NC_000001.11 | 1 | 3632985 | + | WRAP73 | NNNTGAGGAAACAGACACAT | NGG | 2 | 0.3782 | Tier II |
12 | NC_000019.10 | 19 | 39173485 | - | PAK4 | NNNCGATGGGACAGACACAT | NGG | 2 | 0.3595 | Tier II |
13 | NC_000001.11 | 1 | 49690841 | - | AGBL4 | NNNTGATGGAAGAAACACAT | NGG | 2 | 0.3333 | Tier II |
14 | NC_000010.11 | 10 | 21623039 | + | MLLT10 | NNNGGATGGAAGAGACACAT | NGG | 2 | 0.2778 | Tier II |
15 | NC_000001.11 | 1 | 7369538 | + | CAMTA1 | NNNTGAGGGAAGAGACACAT | NGG | 2 | 0.2614 | Tier II |
16 | NC_000016.10 | 16 | 10684135 | + | TEKT5 | NNNTGATGGTACAGACACAT | NAG | 2 | 0.2288 | Tier II |
17 | NC_000018.10 | 18 | 33408762 | + | CCDC178 | NNNTTATGGAAAAGACACAT | NGG | 2 | 0.2143 | Tier II |
18 | NC_000002.12 | 2 | 206448809 | - | ADAM23 | NNNAGATGGAAGAGACACAT | NGG | 2 | 0.2116 | Tier II |
19 | NC_000008.11 | 8 | 135588311 | + | KHDRBS3 | NNNAGATGGAAGAGACACAT | NGG | 2 | 0.2116 | Tier II |
20 | NC_000009.12 | 9 | 17593108 | - | SH3GL2 | NNNAGATGGAAGAGACACAT | NGG | 2 | 0.2116 | Tier II |
21 | NC_000020.11 | 20 | 38830195 | - | PPP1R16B | NNNGGATGGCACAGACACAT | NGG | 2 | 0.2083 | Tier II |
22 | NC_000001.11 | 1 | 246181681 | + | SMYD3 | NNNTTATGAAACAGACACAT | NGG | 2 | 0.1929 | Tier II |
23 | NC_000012.12 | 12 | 120306992 | + | SIRT4 | NNNTGAGGGAACAGATACAT | NGG | 2 | 0.181 | Tier II |
24 | NC_000002.12 | 2 | 100058511 | + | AFF3 | NNNTGATGAAACAGCCACAT | NGG | 2 | 0.1753 | Tier II |
25 | NC_000003.12 | 3 | 184181067 | - | AP2M1 | NNNTGATGGAACAGATAAAT | NGG | 2 | 0.1657 | Tier II |
26 | NC_000009.12 | 9 | 113986050 | + | ZNF618 | NNNTGATGGGACAGACACAT | NAG | 2 | 0.144 | Tier II |
27 | NC_000018.10 | 18 | 46656779 | + | LOXHD1 | NNNATATGGAACAGACACAT | NGG | 2 | 0.1429 | Tier II |
28 | NC_000010.11 | 10 | 125626652 | + | TEX36 | NNNTGATGGAGCAGACACAG | NGG | 2 | 0.1147 | Tier II |
29 | NC_000001.11 | 1 | 160427734 | - | VANGL2 | NNNTGATGGGACAGACACAG | NGG | 2 | 0.098 | Tier II |
30 | NC_000009.12 | 9 | 132571752 | + | CFAP77 | NNNTGATGTAACAGACACAG | NGG | 2 | 0.0941 | Tier II |
31 | NC_000001.11 | 1 | 10014342 | - | RBP7 | NNNTGATGGAAGAGACGCAT | NGG | 2 | 0.0784 | Tier II |
32 | NC_000008.11 | 8 | 102853136 | + | AZIN1 | NNNTGATGGAACACACAGAT | NGG | 2 | 0.0571 | Tier II |
33 | NC_000023.11 | X | 49213218 | + | CACNA1F | NNNTGATGGATCAGAGACAT | NGG | 2 | 0.0473 | Tier II |
34 | NC_000009.12 | 9 | 15891504 | + | CCDC171 | NNNTTATGGAACAGACACAC | NGG | 2 | 0.0273 | Tier II |
35 | NC_000004.12 | 4 | 181700048 | - | TENM3 | NNNTGATGGAACAGCCACAC | NGG | 2 | 0.0248 | Tier II |
36 | NC_000005.10 | 5 | 128382395 | - | FBN2 | NNNTTATGGAACAGACACAT | NGA | 2 | 0.0208 | Tier II |
37 | NC_000011.10 | 11 | 22662045 | - | GAS2 | NNNTTATGGAACAGACACAT | NGA | 2 | 0.0208 | Tier II |
38 | NC_000021.9 | 21 | 6520636 | + | LOC102724652 | NNNTGATGGAACAGACCCAC | NGG | 2 | 0.016 | Tier II |
39 | NC_000010.11 | 10 | 103712454 | - | SH3PXD2A | NNNTGATGGAACAGACACAG | NGA | 2 | 0.0123 | Tier II |
40 | NC_000012.12 | 12 | 127464854 | - | LOC105370066 | NNNTGATGGTACAGACACAA | NGG | 2 | 0.4963 | Tier III |
41 | NC_000004.12 | 4 | 12969702 | + | LOC105374493 | NNNTGATGGAAGAGAAACAT | NGG | 2 | 0.4444 | Tier III |
42 | NC_000011.10 | 11 | 126599571 | - | KIRREL3-AS1 | NNNTGATGGAAAAGACAAAT | NGG | 2 | 0.3846 | Tier III |
43 | NC_000004.12 | 4 | 139210371 | + | LOC105379412 | NNNTGAAGGAACACACACAT | NGG | 2 | 0.375 | Tier III |
44 | NC_000001.11 | 1 | 7369538 | + | CAMTA1-IT1 | NNNTGAGGGAAGAGACACAT | NGG | 2 | 0.2614 | Tier III |
45 | NC_000018.10 | 18 | 47326377 | + | MIR4527HG | NNNTGGTGGAACTGACACAT | NGG | 2 | 0.2143 | Tier III |
46 | NC_000001.11 | 1 | 32876048 | + | LOC105378631 | NNNTGATGGATCAGACATAT | NGG | 2 | 0.1978 | Tier III |
47 | NC_000012.12 | 12 | 93019239 | - | LOC643339 | NNNTGATGGAACTGAGACAT | NGG | 2 | 0.0462 | Tier III |
48 | NC_000012.12 | 12 | 93019239 | - | LINC02413 | NNNTGATGGAACTGAGACAT | NGG | 2 | 0.0462 | Tier III |
49 | NC_000013.11 | 13 | 36709354 | - | LOC102723490 | NNNTGATGCAACAGACACAT | NGA | 2 | 0.0374 | Tier III |
50 | NC_000007.14 | 7 | 94041443 | - | LOC130890646 | NNNTGATGGAACAAACACAT | NTG | 2 | 0.0292 | Tier III |
51 | NC_000021.9 | 21 | 6520636 | + | LOC102724701 | NNNTGATGGAACAGACCCAC | NGG | 2 | 0.016 | Tier III |
52 | NC_000021.9 | 21 | 43128952 | + | LOC101928369 | NNNTGATGGAACAGACCCAC | NGG | 2 | 0.016 | Tier III |
53 | NC_000017.11 | 17 | 79021385 | - | C1QTNF1-AS1 | NNNTGGTGGAACAGACACAT | NGT | 2 | 0.0115 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000069.6 | 3 | 154941077 | + | Tnni3k | NNNGGATGGGACAGACACAT | NGG | 2 | 0.3472 | Tier I |
2 | NC_000070.6 | 4 | 102988585 | + | Tctex1d1 | NNNTCATGGCACAGACACAT | NGG | 2 | 0.2619 | Tier I |
3 | NC_000082.6 | 16 | 97794629 | + | Prdm15 | NNNTGATGGAGCAGTCACAT | NGG | 2 | 0.13 | Tier I |
4 | NC_000071.6 | 5 | 127549835 | + | Tmem132c | NNNTGATGATACAGACACAT | NGG | 2 | 0.5672 | Tier II |
5 | NC_000074.6 | 8 | 95339599 | + | Usb1 | NNNCGATGGAAAAGACACAT | NGG | 2 | 0.4622 | Tier II |
6 | NC_000072.6 | 6 | 53301883 | + | Creb5 | NNNTGATTGAACAGACACTT | NGG | 2 | 0.3365 | Tier II |
7 | NC_000083.6 | 17 | 51102919 | + | Tbc1d5 | NNNTGCTGAAACAGACACAT | NGG | 2 | 0.2922 | Tier II |
8 | NC_000081.6 | 15 | 26860009 | + | Fbxl7 | NNNTGCTGGGACAGACACAT | NGG | 2 | 0.2525 | Tier II |
9 | NC_000067.6 | 1 | 184790829 | + | Mtarc1 | NNNTGATGGAAGAGACACTT | NGG | 2 | 0.2393 | Tier II |
10 | NC_000086.7 | X | 168517362 | - | Frmpd4 | NNNTGGTGGATCAGACACAT | NGG | 2 | 0.2198 | Tier II |
11 | NC_000085.6 | 19 | 47827204 | + | Cfap43 | NNNTTATGAAACAGACACAT | NGG | 2 | 0.1929 | Tier II |
12 | NC_000075.6 | 9 | 63286175 | + | Map2k5 | NNNTGATGAAACAGACACAT | NAG | 2 | 0.1667 | Tier II |
13 | NC_000077.6 | 11 | 119719259 | + | Rptor | NNNTGAAGGAACAGACACAG | NGG | 2 | 0.1544 | Tier II |
14 | NC_000067.6 | 1 | 188458042 | + | Ush2a | NNNTGATGGAACAATCACAT | NGG | 2 | 0.15 | Tier II |
15 | NC_000070.6 | 4 | 74388554 | - | Kdm4c | NNNTGATGGAACAAACACAG | NGG | 2 | 0.1324 | Tier II |
16 | NC_000078.6 | 12 | 29661819 | - | Myt1l | NNNTGATGGAAAAGAGACAT | NGG | 2 | 0.1099 | Tier II |
17 | NC_000070.6 | 4 | 149004592 | - | Pex14 | NNNTTATGGCACAGACACAT | NGG | 2 | 0.1 | Tier II |
18 | NC_000071.6 | 5 | 134121245 | + | Castor2 | NNNTGATGGGACAGACACAG | NGG | 2 | 0.098 | Tier II |
19 | NC_000080.6 | 14 | 27714731 | - | Erc2 | NNNTGATGGCACAGCCACAT | NGG | 2 | 0.0909 | Tier II |
20 | NC_000068.7 | 2 | 19253038 | - | Armc3 | NNNTGATGGAAGAGTCACAT | NGG | 2 | 0.0889 | Tier II |
21 | NC_000083.6 | 17 | 71304202 | + | Emilin2 | NNNAGATGGAACAGACACAG | NGG | 2 | 0.084 | Tier II |
22 | NC_000069.6 | 3 | 69393370 | + | Ppm1l | NNNTGATGGTACAGACACAC | NGG | 2 | 0.0802 | Tier II |
23 | NC_000077.6 | 11 | 53032807 | + | Fstl4 | NNNTGATGGATCAGACACCT | NGG | 2 | 0.0637 | Tier II |
24 | NC_000069.6 | 3 | 158373052 | + | Lrrc7 | NNNAGATGGAACAGACAGAT | NGG | 2 | 0.0635 | Tier II |
25 | NC_000069.6 | 3 | 122247986 | - | Gclm | NNNTGAGGGAACAGACACAC | NGG | 2 | 0.0535 | Tier II |
26 | NC_000069.6 | 3 | 154052279 | + | Slc44a5 | NNNTGATGGAACAGACAGGT | NGG | 2 | 0.05 | Tier II |
27 | NC_000074.6 | 8 | 44957072 | - | Fat1 | NNNTGATGGAACAGCCACAG | NGG | 2 | 0.0481 | Tier II |
28 | NC_000080.6 | 14 | 72861591 | + | Gm4606 | NNNTGATGGAAAAGACACAT | NTG | 2 | 0.0278 | Tier II |
29 | NC_000072.6 | 6 | 9204400 | + | Nxph1 | NNNTGATTGAACAGACACAT | NTG | 2 | 0.0244 | Tier II |
30 | NC_000080.6 | 14 | 46963279 | - | Samd4 | NNNTGATGGAACAGAAACAT | NGT | 2 | 0.0161 | Tier II |
31 | NC_000070.6 | 4 | 102988585 | + | Gm12709 | NNNTCATGGCACAGACACAT | NGG | 2 | 0.2619 | Tier III |
32 | NC_000086.7 | X | 168517362 | - | Gm7209 | NNNTGGTGGATCAGACACAT | NGG | 2 | 0.2198 | Tier III |
33 | NC_000070.6 | 4 | 149004592 | - | Gm42351 | NNNTTATGGCACAGACACAT | NGG | 2 | 0.1 | Tier III |
34 | NC_000068.7 | 2 | 128525050 | + | Gm39928 | NNNTGATGGAAAAGACAGAT | NGG | 2 | 0.0952 | Tier III |
35 | NC_000080.6 | 14 | 27714731 | - | Gm18398 | NNNTGATGGCACAGCCACAT | NGG | 2 | 0.0909 | Tier III |
36 | NC_000076.6 | 10 | 18789073 | + | Gm32926 | NNNTGATGGATCAGAGACAT | NGG | 2 | 0.0473 | Tier III |