Construct: sgRNA BRDN0001145545
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATTCCAGATGAATTACACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K2 (10746)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75509
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
127326761 |
- |
MAP3K2 |
NNNTCCAGATGAATTACACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
183373699 |
- |
MCF2L2 |
NNNTTCAGATGAATTACATC |
NGG |
2 |
0.2937 |
Tier II |
| 3 |
NC_000005.10 |
5 |
91150386 |
+ |
ADGRV1 |
NNNTCAAGATGAATCACACC |
NGG |
2 |
0.2532 |
Tier II |
| 4 |
NC_000006.12 |
6 |
170546366 |
+ |
PSMB1 |
NNNTCCAGATGAATTACAGG |
NGG |
2 |
0.0074 |
Tier II |
| 5 |
NC_000003.12 |
3 |
160079909 |
- |
IL12A-AS1 |
NNNGCCAGATGAATTAAACC |
NGG |
2 |
0.2917 |
Tier III |
| 6 |
NC_000003.12 |
3 |
160087233 |
- |
IL12A-AS1 |
NNNGCCAGATGAATTAAACC |
NGG |
2 |
0.2917 |
Tier III |
| 7 |
NC_000013.11 |
13 |
50348616 |
- |
DLEU1 |
NNNCCCAGATGAACTACACC |
NGG |
2 |
0.1849 |
Tier III |
| 8 |
NC_000005.10 |
5 |
32529619 |
+ |
LOC124900954 |
NNNTCCAGATGAATAACACT |
NGG |
2 |
0.1737 |
Tier III |
| 9 |
NC_000011.10 |
11 |
109787588 |
- |
LINC02715 |
NNNTCCAGATGAATTAGTCC |
NGG |
2 |
0.0294 |
Tier III |
| 10 |
NC_000012.12 |
12 |
3928780 |
- |
LOC105369608 |
NNNTCCAGATGTATGACACC |
NGG |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
21513063 |
+ |
Fbxl13 |
NNNTCAAGATGAATTACATC |
NGG |
2 |
0.4286 |
Tier II |
| 2 |
NC_000083.6 |
17 |
90521339 |
- |
Nrxn1 |
NNNTCCTGATGAATTACACA |
NGG |
2 |
0.2188 |
Tier II |
| 3 |
NC_000067.6 |
1 |
30828004 |
+ |
Phf3 |
NNNTCCAGATCAATTACACA |
NGG |
2 |
0.2143 |
Tier II |
| 4 |
NC_000076.6 |
10 |
43531089 |
+ |
Mtres1 |
NNNTCCAGATGACTGACACC |
NGG |
2 |
0.0105 |
Tier II |
| 5 |
NC_000077.6 |
11 |
119944908 |
- |
Baiap2 |
NNNTCCAGATGAATTACAGC |
NGC |
2 |
0.0028 |
Tier II |
| 6 |
NC_000072.6 |
6 |
127408176 |
+ |
Gm34091 |
NNNTCCAGATGTATTAAACC |
NGG |
2 |
0.1556 |
Tier III |
| 7 |
NC_000085.6 |
19 |
53081382 |
- |
1700054A03Rik |
NNNTCCAGATGAACTACACT |
NGG |
2 |
0.0857 |
Tier III |
| 8 |
NC_000085.6 |
19 |
53081382 |
- |
Gm30234 |
NNNTCCAGATGAACTACACT |
NGG |
2 |
0.0857 |
Tier III |
| 9 |
NC_000072.6 |
6 |
93023036 |
- |
9530026P05Rik |
NNNTGCAGATGAATGACACC |
NGG |
2 |
0.03 |
Tier III |
Other clones with same target sequence:
(none)
