Construct: sgRNA BRDN0001145548

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAGTGGTAGGTATCTCAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRD2 (6046)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77986

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 32976333 - BRD2 NNNGTGGTAGGTATCTCAGG NGG 0 1.0 Tier I
2 NC_000014.9 14 25020618 - STXBP6 NNNGTGGTAGGTGTCTCAGG NGG 1 0.6522 Tier II
3 NC_000002.12 2 154235058 - GALNT13 NNNGTCGTAGGAATCTCAGG NGG 2 0.5455 Tier II
4 NC_000005.10 5 141953052 - RNF14 NNNGTGGCAGGTATCTTAGG NGG 2 0.4667 Tier II
5 NC_000005.10 5 141953052 - PCDH12 NNNGTGGCAGGTATCTTAGG NGG 2 0.4667 Tier II
6 NC_000014.9 14 32488882 + AKAP6 NNNGAGGTAGGTATCTCAGT NGG 2 0.35 Tier II
7 NC_000003.12 3 60161663 + FHIT NNNGAGGTAGGTAACTCAGG NGG 2 0.3095 Tier II
8 NC_000015.10 15 50343508 - GABPB1 NNNGTGGTAGCTATCTTAGG NGG 2 0.2 Tier II
9 NC_000007.14 7 149858789 + ZNF862 NNNGTGGTAGGTATTTCAGG NGG 1 0.0667 Tier II
10 NC_000008.11 8 31087955 - WRN NNNGTGGTAGGTATCACAGG NGA 2 0.0631 Tier II
11 NC_000023.11 X 109394501 - GUCY2F NNNGTGGCAGGTATCTGAGG NGG 2 0.0588 Tier II
12 NC_000022.11 22 23088772 + GNAZ NNNGTGGTAGGTATCTGGGG NGG 2 0.0235 Tier II
13 NC_000022.11 22 23088772 + RSPH14 NNNGTGGTAGGTATCTGGGG NGG 2 0.0235 Tier II
14 NC_000015.10 15 70909513 + LRRC49 NNNGTTGTAGGTATCTCAGG NGC 2 0.0148 Tier II
15 NC_000018.10 18 49368607 - DYM NNNGCGGTAGGTAGCTCAGG NGG 2 0.0 Tier II
16 NC_000023.11 X 19587994 + SH3KBP1 NNNGTGGTAGGTATCTCAGG NCT 2 0.0 Tier II
17 NC_000011.10 11 90700911 - DISC1FP1 NNNGTGGCAAGTATCTCAGG NGG 2 0.9333 Tier III
18 NC_000013.11 13 82823984 + GYG1P2 NNNGTGGAAGATATCTCAGG NGG 2 0.8 Tier III
19 NC_000006.12 6 140833160 - LOC102723724 NNNGTGATAGGTATCTCAGG NTG 2 0.039 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 34115494 + Brd2 NNNGTGGTAGGTATTTCAGG NGG 1 0.0667 Tier I
2 NC_000069.6 3 19646018 + Trim55 NNNGTGGCTGGTATCTCAGG NGG 2 0.6 Tier II
3 NC_000076.6 10 52277688 - Dcbld1 NNNGTGGCTGGTATCTCAGG NGG 2 0.6 Tier II
4 NC_000084.6 18 39224206 + Arhgap26 NNNGTGGAAGTTATCTCAGG NGG 2 0.3077 Tier II
5 NC_000085.6 19 41226979 - Tm9sf3 NNNGTGGTAGATTTCTCAGG NGG 2 0.3 Tier II
6 NC_000077.6 11 51577172 + Col23a1 NNNGTGGTAGGTGTCTCAGC NGG 2 0.2795 Tier II
7 NC_000077.6 11 87542037 - Tex14 NNNGTGGTGCGTATCTCAGG NGG 2 0.2667 Tier II
8 NC_000068.7 2 153841492 - Efcab8 NNNGTGGTTGGTACCTCAGG NGG 2 0.1714 Tier II
9 NC_000073.6 7 35438904 - Cep89 NNNGTGGTAGGTCACTCAGG NGG 2 0.1303 Tier II
10 NC_000070.6 4 150861956 + Errfi1 NNNGTGGTAGGTACCTCAGG NAG 2 0.0741 Tier II
11 NC_000086.7 X 73263069 + Xlr3c NNNGTGGTAGGAATTTCAGG NGG 2 0.0533 Tier II
12 NC_000073.6 7 112249411 - Mical2 NNNGTGGGAGGTATCTCAGG NTG 2 0.0286 Tier II
13 NC_000078.6 12 102650733 + Itpk1 NNNGAGGTAGGTATCTCAGG NGC 2 0.0111 Tier II
14 NC_000071.6 5 34313896 + Cfap99 NNNGTGGTAGGGAGCTCAGG NGG 2 0.0 Tier II
15 NC_000070.6 4 125911420 + 2610028E06Rik NNNGTGGAAGGTCTCTCAGG NGG 2 0.1684 Tier III
16 NC_000073.6 7 35438904 - Gm42378 NNNGTGGTAGGTCACTCAGG NGG 2 0.1303 Tier III
17 NC_000067.6 1 170254950 - Gm6345 NNNGTGGTTGGTCTCTCAGG NGG 2 0.1263 Tier III
18 NC_000086.7 X 81585805 + LOC115489112 NNNGTGGTTGGTCTCTCAGG NGG 2 0.1263 Tier III
19 NC_000086.7 X 74912767 - Xlr3e-ps NNNGTGGTAGGAATTTCAGG NGG 2 0.0533 Tier III
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Other clones with same target sequence:

(none)