Construct: sgRNA BRDN0001145550
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAGCGTGGATAATAACTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRPM6 (140803)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77337
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
74840041 |
- |
TRPM6 |
NNNGCGTGGATAATAACTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
95113422 |
+ |
BMPR1B |
NNNGTGTTGATAATAACTGA |
NGG |
2 |
0.3977 |
Tier II |
| 3 |
NC_000017.11 |
17 |
67076198 |
+ |
HELZ |
NNNGGGTGGATAATCACTGA |
NGG |
2 |
0.1636 |
Tier II |
| 4 |
NC_000009.12 |
9 |
24086681 |
+ |
LOC124902327 |
NNNGCATGGATAATAACTGA |
NGA |
2 |
0.0694 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
51298996 |
+ |
Col23a1 |
NNNGAGTGGAAAATAACTGA |
NGG |
2 |
0.4286 |
Tier II |
| 2 |
NC_000068.7 |
2 |
28581493 |
- |
Gtf3c5 |
NNNGTGTGGATAAGAACTGA |
NGG |
2 |
0.0 |
Tier II |
| 3 |
NC_000068.7 |
2 |
71734798 |
- |
Gm17250 |
NNNGCCTGGATAATGACTGA |
NGG |
2 |
0.4432 |
Tier III |
Other clones with same target sequence:
(none)
