Construct: sgRNA BRDN0001145554

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCATTAAGCAGATCCTGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAPK2 (23604)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76019

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 63971474 - DAPK2 NNNTTAAGCAGATCCTGGAT NGG 0 1.0 Tier I
2 NC_000005.10 5 146131864 - LARS1 NNNTTAAGAAGAACCTGGAT NGG 2 0.5934 Tier II
3 NC_000003.12 3 12389013 - PPARG NNNTCCAGCAGATCCTGGAT NGG 2 0.4545 Tier II
4 NC_000001.11 1 236447770 - EDARADD NNNTTAAGCAGATCATAGAT NGG 2 0.2074 Tier II
5 NC_000014.9 14 39148059 + TRAPPC6B NNNTAAAGCAGTTCCTGGAT NGG 2 0.1667 Tier II
6 NC_000010.11 10 18277901 + CACNB2 NNNTTAAGCAGATCATGTAT NGG 2 0.1481 Tier II
7 NC_000021.9 21 45871858 + PCBP3 NNNTTAAGCAGCTCCTGGAA NGG 2 0.148 Tier II
8 NC_000014.9 14 22611411 - ABHD4 NNNTTAAGCAGACCCTGGAT NGA 2 0.0548 Tier II
9 NC_000018.10 18 48942190 - SMAD7 NNNTTAAGCAGCTCCTGGCT NGG 2 0.0544 Tier II
10 NC_000022.11 22 45209883 + KIAA0930 NNNTTCAGCAGATCCTGGAT NTG 2 0.0177 Tier II
11 NC_000001.11 1 52414211 + PRPF38A NNNTTAAGCAGAGCTTGGAT NGG 2 0.0174 Tier II
12 NC_000007.14 7 7796707 + UMAD1 NNNTTAAGCAGATCCTGGAT NCT 2 0.0 Tier II
13 NC_000009.12 9 19856779 - SLC24A2 NNNTTAAGCAGATCCTGGAT NCA 2 0.0 Tier II
14 NC_000009.12 9 119329494 + BRINP1 NNNTTAAGCAGATGCTGGAT NTG 2 0.0 Tier II
15 NC_000006.12 6 30308238 + HCG18 NNNTTAAGAAGATTCTGGAT NGG 2 0.6286 Tier III
16 NC_000006.12 6 30308238 + HCG17 NNNTTAAGAAGATTCTGGAT NGG 2 0.6286 Tier III
17 NC_000017.11 17 59447188 + LINC01476 NNNTTGAGTAGATCCTGGAT NGG 2 0.625 Tier III
18 NC_000017.11 17 59447188 + LOC124904040 NNNTTGAGTAGATCCTGGAT NGG 2 0.625 Tier III
19 NC_000004.12 4 187675888 + LOC107983963 NNNTTTAGCAGATTCTGGAT NGG 2 0.5238 Tier III
20 NC_000014.9 14 101553809 - DIO3OS NNNCTGAGCAGATCCTGGAT NGG 2 0.4622 Tier III
21 NC_000023.11 X 74145199 + FTX NNNTTAAGCAGATCCAGCAT NGG 2 0.4329 Tier III
22 NC_000001.11 1 55232665 + MIR4422HG NNNTTAACCAGATCCTGGAG NGG 2 0.1086 Tier III
23 NC_000004.12 4 23348623 + LOC105374523 NNNTTCAGCAGATCTTGGAT NGG 2 0.0303 Tier III
24 NC_000004.12 4 23348623 + LOC105374524 NNNTTCAGCAGATCTTGGAT NGG 2 0.0303 Tier III
25 NC_000008.11 8 18035266 + LOC124901894 NNNTTAAGCAGATCCTGGAA NGT 2 0.0091 Tier III
26 NC_000007.14 7 7796707 + LOC105379719 NNNTTAAGCAGATCCTGGAT NCT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 66231801 + Dapk2 NNNTTAAGCAGATCCTGGAT NGG 0 1.0 Tier I
2 NC_000073.6 7 35028153 + Pepd NNNTTAAGCAGGACCTGGAT NGG 2 0.5 Tier II
3 NC_000067.6 1 185468233 + Slc30a10 NNNTTAAGCAGAACCTGTAT NGG 2 0.4615 Tier II
4 NC_000080.6 14 115099834 + Gpc5 NNNTTGATCAGATCCTGGAT NGG 2 0.4464 Tier II
5 NC_000072.6 6 55126043 - Crhr2 NNNTCAAGCAGATCCTGGGT NGG 2 0.375 Tier II
6 NC_000077.6 11 5437581 - Znrf3 NNNTTCACCAGATCCTGGAT NGG 2 0.2797 Tier II
7 NC_000078.6 12 89080348 - Nrxn3 NNNTTAAGCAGATCCTGCAA NGG 2 0.2679 Tier II
8 NC_000070.6 4 130256124 - Serinc2 NNNTTAAGCAGCTCCTGGTT NGG 2 0.1417 Tier II
9 NC_000068.7 2 27201089 + Sardh NNNTGAAGCAGATCCTGGAG NGG 2 0.1129 Tier II
10 NC_000068.7 2 120072765 - Sptbn5 NNNATAAGCAGATCCTGGAG NGG 2 0.084 Tier II
11 NC_000086.7 X 157208173 - Phex NNNTTAAGCAGATTGTGGAT NGG 2 0.0367 Tier II
12 NC_000074.6 8 81985819 + Inpp4b NNNTAAAGCAGATCCTGGAT NTG 2 0.0195 Tier II
13 NC_000070.6 4 117059529 - Eif2b3 NNNTTAAGCAGACCCTGGAT NGT 2 0.0127 Tier II
14 NC_000067.6 1 70914192 - Gm29906 NNNTTTAGCAGATTCTGGAT NGG 2 0.5238 Tier III
15 NC_000073.6 7 35028153 + Gm35484 NNNTTAAGCAGGACCTGGAT NGG 2 0.5 Tier III
16 NC_000073.6 7 24827246 - Gm5893 NNNTTATCCAGATCCTGGAT NGG 2 0.2692 Tier III
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Other clones with same target sequence:

(none)