Construct: sgRNA BRDN0001145557
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAGATGCTCGAGGACTCATA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NUAK1 (9891)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75575
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
106067942 |
- |
NUAK1 |
NNNATGCTCGAGGACTCATA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
33324869 |
- |
PHC2 |
NNNATGCTCAAGGACTCCTA |
NGG |
2 |
0.1778 |
Tier I |
| 3 |
NC_000017.11 |
17 |
67863993 |
- |
BPTF |
NNNATTCTCAAGGACTCATA |
NGG |
2 |
0.6222 |
Tier II |
| 4 |
NC_000012.12 |
12 |
95077886 |
+ |
FGD6 |
NNNATGCTTGAGGACTCATA |
NAG |
2 |
0.2269 |
Tier II |
| 5 |
NC_000014.9 |
14 |
73901045 |
- |
ZNF410 |
NNNATGCTAGAGGACTCATA |
NAG |
2 |
0.2222 |
Tier II |
| 6 |
NC_000015.10 |
15 |
61519323 |
- |
LOC107984782 |
NNNATGCTGGAGGAATCATA |
NGG |
2 |
0.1376 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
84375374 |
- |
Nuak1 |
NNNATGCTCGGGGACTCATC |
NGG |
2 |
0.1477 |
Tier I |
| 2 |
NC_000078.6 |
12 |
116691133 |
- |
Ptprn2 |
NNNATACTCTAGGACTCATA |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000074.6 |
8 |
69094727 |
- |
Atp6v1b2 |
NNNAAGCTCCAGGACTCATA |
NGG |
2 |
0.2 |
Tier II |
Other clones with same target sequence:
(none)
