Construct: sgRNA BRDN0001145564
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGCCACTGTGTGATCACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FLT1 (2321)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75557
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000013.11 |
13 |
28384998 |
+ |
FLT1 |
NNNCCACTGTGTGATCACTG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000019.10 |
19 |
8094659 |
+ |
FBN3 |
NNNTCACTGTGTGATCACTG |
NGG |
1 |
0.8 |
Tier II |
3 |
NC_000010.11 |
10 |
14707567 |
+ |
FAM107B |
NNNCCACTTTGTGATCACTG |
NGG |
1 |
0.5333 |
Tier II |
4 |
NC_000018.10 |
18 |
33725853 |
- |
ASXL3 |
NNNGCACTGAGTGATCACTG |
NGG |
2 |
0.4286 |
Tier II |
5 |
NC_000006.12 |
6 |
161978700 |
+ |
PRKN |
NNNCCACTGTGAGGTCACTG |
NGG |
2 |
0.3733 |
Tier II |
6 |
NC_000012.12 |
12 |
132052255 |
- |
EP400 |
NNNCCACTGTGTGACAACTG |
NGG |
2 |
0.2727 |
Tier II |
7 |
NC_000003.12 |
3 |
62708768 |
+ |
CADPS |
NNNCCACTGTGGGATCAATG |
NGG |
2 |
0.2692 |
Tier II |
8 |
NC_000008.11 |
8 |
130187834 |
- |
ASAP1 |
NNNCCACAGTGTGACCACTG |
NGG |
2 |
0.2182 |
Tier II |
9 |
NC_000011.10 |
11 |
7602451 |
+ |
PPFIBP2 |
NNNCCACTGTGAGCTCACTG |
NGG |
2 |
0.1714 |
Tier II |
10 |
NC_000007.14 |
7 |
75653793 |
+ |
HIP1 |
NNNCCACTGAGTGATCGCTG |
NGG |
2 |
0.1513 |
Tier II |
11 |
NC_000021.9 |
21 |
44504136 |
- |
TSPEAR |
NNNCCACTGTGGGCTCACTG |
NGG |
2 |
0.1071 |
Tier II |
12 |
NC_000021.9 |
21 |
44504440 |
- |
TSPEAR |
NNNCCACTGTGGGCTCACTG |
NGG |
2 |
0.1071 |
Tier II |
13 |
NC_000002.12 |
2 |
101117075 |
+ |
TBC1D8 |
NNNGCACTGTGTGATGACTG |
NGG |
2 |
0.0769 |
Tier II |
14 |
NC_000010.11 |
10 |
77052164 |
- |
KCNMA1 |
NNNCCACTGTATGATCACTG |
NTG |
2 |
0.039 |
Tier II |
15 |
NC_000019.10 |
19 |
50513198 |
- |
ASPDH |
NNNCCACTGTGTGATCTCAG |
NGG |
2 |
0.0381 |
Tier II |
16 |
NC_000001.11 |
1 |
39455123 |
+ |
MACF1 |
NNNTCACTGTGTGATCACTG |
NTG |
2 |
0.0312 |
Tier II |
17 |
NC_000013.11 |
13 |
110312702 |
+ |
COL4A2 |
NNNCTACTGTGTGATCACTG |
NTG |
2 |
0.0248 |
Tier II |
18 |
NC_000009.12 |
9 |
127824494 |
+ |
ENG |
NNNTCACTGTGTGATCACTG |
NGT |
2 |
0.0129 |
Tier II |
19 |
NC_000001.11 |
1 |
67712003 |
+ |
GNG12 |
NNNCCACTGTGTGATCCCTG |
NGA |
2 |
0.0123 |
Tier II |
20 |
NC_000015.10 |
15 |
30662463 |
+ |
LOC100288637 |
NNNCCACTGTGTAATCATTG |
NGG |
2 |
0.5934 |
Tier III |
21 |
NC_000014.9 |
14 |
105913630 |
+ |
IGH |
NNNCCACTGTGATATCACTG |
NGG |
2 |
0.24 |
Tier III |
22 |
NC_000002.12 |
2 |
25014680 |
- |
DNAJC27-AS1 |
NNNCAACTGTGTGATCACAG |
NGG |
2 |
0.1633 |
Tier III |
23 |
NC_000005.10 |
5 |
38646500 |
+ |
LIFR-AS1 |
NNNCCACTGTGTAAGCACTG |
NGG |
2 |
0.0462 |
Tier III |
24 |
NC_000019.10 |
19 |
50513198 |
- |
LOC124904747 |
NNNCCACTGTGTGATCTCAG |
NGG |
2 |
0.0381 |
Tier III |
25 |
NC_000008.11 |
8 |
48439054 |
+ |
LOC105375821 |
NNNCCACTGTGTGCTCCCTG |
NGG |
2 |
0.0378 |
Tier III |
26 |
NC_000022.11 |
22 |
22026548 |
- |
IGL |
NNNGCACTGTGTGAGCACTG |
NGG |
2 |
0.025 |
Tier III |
27 |
NC_000022.11 |
22 |
22026548 |
- |
IGLVI-70 |
NNNGCACTGTGTGAGCACTG |
NGG |
2 |
0.025 |
Tier III |
28 |
NC_000022.11 |
22 |
22026548 |
- |
PRAMENP |
NNNGCACTGTGTGAGCACTG |
NGG |
2 |
0.025 |
Tier III |
29 |
NC_000022.11 |
22 |
22215266 |
- |
IGL |
NNNGCACTGTGTGAGCACTG |
NGG |
2 |
0.025 |
Tier III |
30 |
NC_000022.11 |
22 |
22215266 |
- |
IGLV10-54 |
NNNGCACTGTGTGAGCACTG |
NGG |
2 |
0.025 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000075.6 |
9 |
107298273 |
- |
Cish |
NNNCCACTGTGTCATCACGG |
NGG |
2 |
0.1053 |
Tier I |
2 |
NC_000083.6 |
17 |
33935437 |
- |
Rgl2 |
NNNCCACTGTGTGATCACTA |
NTG |
2 |
0.0365 |
Tier I |
3 |
NC_000080.6 |
14 |
13359547 |
+ |
Synpr |
NNNCCACGGTGTGATCACTG |
NGG |
1 |
0.7333 |
Tier II |
4 |
NC_000073.6 |
7 |
84588363 |
- |
Fah |
NNNCCACAGTGTGATCATTG |
NGG |
2 |
0.5143 |
Tier II |
5 |
NC_000076.6 |
10 |
4009903 |
- |
Mthfd1l |
NNNCCACTGTGTGTTCACTA |
NGG |
2 |
0.5 |
Tier II |
6 |
NC_000080.6 |
14 |
31242286 |
+ |
Phf7 |
NNNCCATTGTGTGAACACTG |
NGG |
2 |
0.4704 |
Tier II |
7 |
NC_000078.6 |
12 |
83447352 |
- |
Dpf3 |
NNNCCACTGTGTGGTCACTA |
NGG |
2 |
0.4375 |
Tier II |
8 |
NC_000070.6 |
4 |
84525856 |
- |
Bnc2 |
NNNACACTGTGGGATCACTG |
NGG |
2 |
0.4211 |
Tier II |
9 |
NC_000078.6 |
12 |
113058408 |
+ |
Pacs2 |
NNNGCAATGTGTGATCACTG |
NGG |
2 |
0.375 |
Tier II |
10 |
NC_000086.7 |
X |
13660943 |
- |
Cask |
NNNCCACTGTGTGTTCATTG |
NGG |
2 |
0.3429 |
Tier II |
11 |
NC_000086.7 |
X |
13660943 |
- |
Gpr82 |
NNNCCACTGTGTGTTCATTG |
NGG |
2 |
0.3429 |
Tier II |
12 |
NC_000072.6 |
6 |
117916368 |
+ |
Hnrnpf |
NNNCCACTATGTGGTCACTG |
NGG |
2 |
0.3 |
Tier II |
13 |
NC_000074.6 |
8 |
104228950 |
+ |
Tk2 |
NNNCCACTGTGTGACAACTG |
NGG |
2 |
0.2727 |
Tier II |
14 |
NT_166281.1 |
1 |
166736 |
- |
LOC100041057 |
NNNCGCCTGTGTGATCACTG |
NGG |
2 |
0.2727 |
Tier II |
15 |
NC_000081.6 |
15 |
48091089 |
+ |
Csmd3 |
NNNCCACTGTGTGGACACTG |
NGG |
2 |
0.2702 |
Tier II |
16 |
NC_000071.6 |
5 |
27460336 |
- |
Dpp6 |
NNNCCACAGTGTGCTCACTG |
NGG |
2 |
0.1714 |
Tier II |
17 |
NC_000068.7 |
2 |
13100373 |
- |
Rsu1 |
NNNTCACTGTGTGCTCACTG |
NGG |
2 |
0.1714 |
Tier II |
18 |
NC_000073.6 |
7 |
67520047 |
+ |
Lrrc28 |
NNNTCACTGTGTGCTCACTG |
NGG |
2 |
0.1714 |
Tier II |
19 |
NC_000076.6 |
10 |
60448086 |
- |
Cdh23 |
NNNCCACTGTTTGATCACTC |
NGG |
2 |
0.1648 |
Tier II |
20 |
NC_000070.6 |
4 |
82902329 |
- |
Frem1 |
NNNCCACTCTGTGATCACAG |
NGG |
2 |
0.1538 |
Tier II |
21 |
NC_000085.6 |
19 |
7295753 |
- |
Mark2 |
NNNCCACTGTGGGATTACTG |
NGG |
2 |
0.1538 |
Tier II |
22 |
NC_000072.6 |
6 |
144066144 |
+ |
Sox5 |
NNNCCACTGAGTGATCAGTG |
NGG |
2 |
0.1143 |
Tier II |
23 |
NC_000071.6 |
5 |
149377243 |
- |
Medag |
NNNCCACTGTGTGTTCGCTG |
NGG |
2 |
0.0941 |
Tier II |
24 |
NC_000074.6 |
8 |
88674692 |
+ |
Nod2 |
NNNCCCCTGTGTGATCCCTG |
NGG |
2 |
0.0802 |
Tier II |
25 |
NC_000075.6 |
9 |
21173682 |
+ |
Pde4a |
NNNCCACCGTGTGATCACTG |
NGA |
2 |
0.0694 |
Tier II |
26 |
NC_000071.6 |
5 |
150366410 |
+ |
Fry |
NNNCCACTGTGTGATCACTG |
NGA |
1 |
0.0694 |
Tier II |
27 |
NC_000074.6 |
8 |
43140323 |
- |
Triml1 |
NNNCCACTGTGTGCTCACCG |
NGG |
2 |
0.0591 |
Tier II |
28 |
NC_000067.6 |
1 |
134220695 |
- |
Adora1 |
NNNCCACTGTGTGATCTCTC |
NGG |
2 |
0.0571 |
Tier II |
29 |
NC_000071.6 |
5 |
117550814 |
+ |
Ksr2 |
NNNCCACTGTGTCATCTCTG |
NGG |
2 |
0.0561 |
Tier II |
30 |
NC_000084.6 |
18 |
77660561 |
- |
8030462N17Rik |
NNNCCACTGTGTGAGTACTG |
NGG |
2 |
0.0154 |
Tier II |
31 |
NC_000072.6 |
6 |
32579418 |
+ |
Plxna4 |
NNNGCACTGTGTGATCACTG |
NGC |
2 |
0.0111 |
Tier II |
32 |
NC_000071.6 |
5 |
121598456 |
- |
Acad12 |
NNNCCACTGTGTGACCACTG |
NTG |
2 |
0.0106 |
Tier II |
33 |
NC_000074.6 |
8 |
17437248 |
- |
C030002A05Rik |
NNNCCACTGTGTGAGCACTG |
NGA |
2 |
0.0035 |
Tier II |
34 |
NC_000074.6 |
8 |
17437248 |
- |
Csmd1 |
NNNCCACTGTGTGAGCACTG |
NGA |
2 |
0.0035 |
Tier II |
35 |
NC_000081.6 |
15 |
92541260 |
+ |
Pdzrn4 |
NNNCCACTGTGTGAGCACTG |
NGA |
2 |
0.0035 |
Tier II |
36 |
NC_000078.6 |
12 |
30280076 |
- |
Sntg2 |
NNNCCACTGTGTGCTCACTG |
NGT |
2 |
0.0035 |
Tier II |
37 |
NC_000072.6 |
6 |
17147260 |
- |
Gm4876 |
NNNTCACAGTGTGATCACTG |
NGG |
2 |
0.64 |
Tier III |
38 |
NC_000069.6 |
3 |
38761884 |
- |
Gm29959 |
NNNCCACCGTGTGTTCACTG |
NGG |
2 |
0.5333 |
Tier III |
39 |
NC_000071.6 |
5 |
28666642 |
+ |
9530036O11Rik |
NNNCCACAGTGGGATCACTG |
NGG |
2 |
0.4 |
Tier III |
40 |
NC_000080.6 |
14 |
99523949 |
- |
Gm41230 |
NNNCCCCTGTGGGATCACTG |
NGG |
2 |
0.2273 |
Tier III |
41 |
NC_000085.6 |
19 |
59660075 |
+ |
Gm19956 |
NNNCCACTGTGTGTTCCCTG |
NGG |
2 |
0.0941 |
Tier III |
42 |
NC_000070.6 |
4 |
45730391 |
- |
Gm31563 |
NNNCCACTGTGTGACCACAG |
NGG |
2 |
0.0779 |
Tier III |
43 |
NC_000081.6 |
15 |
74200947 |
- |
Gm32918 |
NNNCCACTGTGTGATTCCTG |
NGG |
2 |
0.0543 |
Tier III |
44 |
NC_000068.7 |
2 |
105508009 |
+ |
4930527A07Rik |
NNNCCAATGTGTGATCACTG |
NGA |
2 |
0.0521 |
Tier III |
45 |
NC_000082.6 |
16 |
46023413 |
+ |
Gm31198 |
NNNCCACTGTGTGATTTCTG |
NGG |
2 |
0.041 |
Tier III |
46 |
NC_000072.6 |
6 |
12200678 |
- |
Gm35961 |
NNNCCACTGTCTGAGCACTG |
NGG |
2 |
0.0214 |
Tier III |
47 |
NC_000077.6 |
11 |
5500685 |
- |
Gm11963 |
NNNCCACTGTGTGAACACTG |
NGC |
2 |
0.0129 |
Tier III |
Other clones with same target sequence:
(none)