Construct: sgRNA BRDN0001145564

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGCCACTGTGTGATCACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLT1 (2321)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75557

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000013.11 13 28384998 + FLT1 NNNCCACTGTGTGATCACTG NGG 0 1.0 Tier I
2 NC_000019.10 19 8094659 + FBN3 NNNTCACTGTGTGATCACTG NGG 1 0.8 Tier II
3 NC_000010.11 10 14707567 + FAM107B NNNCCACTTTGTGATCACTG NGG 1 0.5333 Tier II
4 NC_000018.10 18 33725853 - ASXL3 NNNGCACTGAGTGATCACTG NGG 2 0.4286 Tier II
5 NC_000006.12 6 161978700 + PRKN NNNCCACTGTGAGGTCACTG NGG 2 0.3733 Tier II
6 NC_000012.12 12 132052255 - EP400 NNNCCACTGTGTGACAACTG NGG 2 0.2727 Tier II
7 NC_000003.12 3 62708768 + CADPS NNNCCACTGTGGGATCAATG NGG 2 0.2692 Tier II
8 NC_000008.11 8 130187834 - ASAP1 NNNCCACAGTGTGACCACTG NGG 2 0.2182 Tier II
9 NC_000011.10 11 7602451 + PPFIBP2 NNNCCACTGTGAGCTCACTG NGG 2 0.1714 Tier II
10 NC_000007.14 7 75653793 + HIP1 NNNCCACTGAGTGATCGCTG NGG 2 0.1513 Tier II
11 NC_000021.9 21 44504136 - TSPEAR NNNCCACTGTGGGCTCACTG NGG 2 0.1071 Tier II
12 NC_000021.9 21 44504440 - TSPEAR NNNCCACTGTGGGCTCACTG NGG 2 0.1071 Tier II
13 NC_000002.12 2 101117075 + TBC1D8 NNNGCACTGTGTGATGACTG NGG 2 0.0769 Tier II
14 NC_000010.11 10 77052164 - KCNMA1 NNNCCACTGTATGATCACTG NTG 2 0.039 Tier II
15 NC_000019.10 19 50513198 - ASPDH NNNCCACTGTGTGATCTCAG NGG 2 0.0381 Tier II
16 NC_000001.11 1 39455123 + MACF1 NNNTCACTGTGTGATCACTG NTG 2 0.0312 Tier II
17 NC_000013.11 13 110312702 + COL4A2 NNNCTACTGTGTGATCACTG NTG 2 0.0248 Tier II
18 NC_000009.12 9 127824494 + ENG NNNTCACTGTGTGATCACTG NGT 2 0.0129 Tier II
19 NC_000001.11 1 67712003 + GNG12 NNNCCACTGTGTGATCCCTG NGA 2 0.0123 Tier II
20 NC_000015.10 15 30662463 + LOC100288637 NNNCCACTGTGTAATCATTG NGG 2 0.5934 Tier III
21 NC_000014.9 14 105913630 + IGH NNNCCACTGTGATATCACTG NGG 2 0.24 Tier III
22 NC_000002.12 2 25014680 - DNAJC27-AS1 NNNCAACTGTGTGATCACAG NGG 2 0.1633 Tier III
23 NC_000005.10 5 38646500 + LIFR-AS1 NNNCCACTGTGTAAGCACTG NGG 2 0.0462 Tier III
24 NC_000019.10 19 50513198 - LOC124904747 NNNCCACTGTGTGATCTCAG NGG 2 0.0381 Tier III
25 NC_000008.11 8 48439054 + LOC105375821 NNNCCACTGTGTGCTCCCTG NGG 2 0.0378 Tier III
26 NC_000022.11 22 22026548 - IGL NNNGCACTGTGTGAGCACTG NGG 2 0.025 Tier III
27 NC_000022.11 22 22026548 - IGLVI-70 NNNGCACTGTGTGAGCACTG NGG 2 0.025 Tier III
28 NC_000022.11 22 22026548 - PRAMENP NNNGCACTGTGTGAGCACTG NGG 2 0.025 Tier III
29 NC_000022.11 22 22215266 - IGL NNNGCACTGTGTGAGCACTG NGG 2 0.025 Tier III
30 NC_000022.11 22 22215266 - IGLV10-54 NNNGCACTGTGTGAGCACTG NGG 2 0.025 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 107298273 - Cish NNNCCACTGTGTCATCACGG NGG 2 0.1053 Tier I
2 NC_000083.6 17 33935437 - Rgl2 NNNCCACTGTGTGATCACTA NTG 2 0.0365 Tier I
3 NC_000080.6 14 13359547 + Synpr NNNCCACGGTGTGATCACTG NGG 1 0.7333 Tier II
4 NC_000073.6 7 84588363 - Fah NNNCCACAGTGTGATCATTG NGG 2 0.5143 Tier II
5 NC_000076.6 10 4009903 - Mthfd1l NNNCCACTGTGTGTTCACTA NGG 2 0.5 Tier II
6 NC_000080.6 14 31242286 + Phf7 NNNCCATTGTGTGAACACTG NGG 2 0.4704 Tier II
7 NC_000078.6 12 83447352 - Dpf3 NNNCCACTGTGTGGTCACTA NGG 2 0.4375 Tier II
8 NC_000070.6 4 84525856 - Bnc2 NNNACACTGTGGGATCACTG NGG 2 0.4211 Tier II
9 NC_000078.6 12 113058408 + Pacs2 NNNGCAATGTGTGATCACTG NGG 2 0.375 Tier II
10 NC_000086.7 X 13660943 - Cask NNNCCACTGTGTGTTCATTG NGG 2 0.3429 Tier II
11 NC_000086.7 X 13660943 - Gpr82 NNNCCACTGTGTGTTCATTG NGG 2 0.3429 Tier II
12 NC_000072.6 6 117916368 + Hnrnpf NNNCCACTATGTGGTCACTG NGG 2 0.3 Tier II
13 NC_000074.6 8 104228950 + Tk2 NNNCCACTGTGTGACAACTG NGG 2 0.2727 Tier II
14 NT_166281.1 1 166736 - LOC100041057 NNNCGCCTGTGTGATCACTG NGG 2 0.2727 Tier II
15 NC_000081.6 15 48091089 + Csmd3 NNNCCACTGTGTGGACACTG NGG 2 0.2702 Tier II
16 NC_000071.6 5 27460336 - Dpp6 NNNCCACAGTGTGCTCACTG NGG 2 0.1714 Tier II
17 NC_000068.7 2 13100373 - Rsu1 NNNTCACTGTGTGCTCACTG NGG 2 0.1714 Tier II
18 NC_000073.6 7 67520047 + Lrrc28 NNNTCACTGTGTGCTCACTG NGG 2 0.1714 Tier II
19 NC_000076.6 10 60448086 - Cdh23 NNNCCACTGTTTGATCACTC NGG 2 0.1648 Tier II
20 NC_000070.6 4 82902329 - Frem1 NNNCCACTCTGTGATCACAG NGG 2 0.1538 Tier II
21 NC_000085.6 19 7295753 - Mark2 NNNCCACTGTGGGATTACTG NGG 2 0.1538 Tier II
22 NC_000072.6 6 144066144 + Sox5 NNNCCACTGAGTGATCAGTG NGG 2 0.1143 Tier II
23 NC_000071.6 5 149377243 - Medag NNNCCACTGTGTGTTCGCTG NGG 2 0.0941 Tier II
24 NC_000074.6 8 88674692 + Nod2 NNNCCCCTGTGTGATCCCTG NGG 2 0.0802 Tier II
25 NC_000075.6 9 21173682 + Pde4a NNNCCACCGTGTGATCACTG NGA 2 0.0694 Tier II
26 NC_000071.6 5 150366410 + Fry NNNCCACTGTGTGATCACTG NGA 1 0.0694 Tier II
27 NC_000074.6 8 43140323 - Triml1 NNNCCACTGTGTGCTCACCG NGG 2 0.0591 Tier II
28 NC_000067.6 1 134220695 - Adora1 NNNCCACTGTGTGATCTCTC NGG 2 0.0571 Tier II
29 NC_000071.6 5 117550814 + Ksr2 NNNCCACTGTGTCATCTCTG NGG 2 0.0561 Tier II
30 NC_000084.6 18 77660561 - 8030462N17Rik NNNCCACTGTGTGAGTACTG NGG 2 0.0154 Tier II
31 NC_000072.6 6 32579418 + Plxna4 NNNGCACTGTGTGATCACTG NGC 2 0.0111 Tier II
32 NC_000071.6 5 121598456 - Acad12 NNNCCACTGTGTGACCACTG NTG 2 0.0106 Tier II
33 NC_000074.6 8 17437248 - C030002A05Rik NNNCCACTGTGTGAGCACTG NGA 2 0.0035 Tier II
34 NC_000074.6 8 17437248 - Csmd1 NNNCCACTGTGTGAGCACTG NGA 2 0.0035 Tier II
35 NC_000081.6 15 92541260 + Pdzrn4 NNNCCACTGTGTGAGCACTG NGA 2 0.0035 Tier II
36 NC_000078.6 12 30280076 - Sntg2 NNNCCACTGTGTGCTCACTG NGT 2 0.0035 Tier II
37 NC_000072.6 6 17147260 - Gm4876 NNNTCACAGTGTGATCACTG NGG 2 0.64 Tier III
38 NC_000069.6 3 38761884 - Gm29959 NNNCCACCGTGTGTTCACTG NGG 2 0.5333 Tier III
39 NC_000071.6 5 28666642 + 9530036O11Rik NNNCCACAGTGGGATCACTG NGG 2 0.4 Tier III
40 NC_000080.6 14 99523949 - Gm41230 NNNCCCCTGTGGGATCACTG NGG 2 0.2273 Tier III
41 NC_000085.6 19 59660075 + Gm19956 NNNCCACTGTGTGTTCCCTG NGG 2 0.0941 Tier III
42 NC_000070.6 4 45730391 - Gm31563 NNNCCACTGTGTGACCACAG NGG 2 0.0779 Tier III
43 NC_000081.6 15 74200947 - Gm32918 NNNCCACTGTGTGATTCCTG NGG 2 0.0543 Tier III
44 NC_000068.7 2 105508009 + 4930527A07Rik NNNCCAATGTGTGATCACTG NGA 2 0.0521 Tier III
45 NC_000082.6 16 46023413 + Gm31198 NNNCCACTGTGTGATTTCTG NGG 2 0.041 Tier III
46 NC_000072.6 6 12200678 - Gm35961 NNNCCACTGTCTGAGCACTG NGG 2 0.0214 Tier III
47 NC_000077.6 11 5500685 - Gm11963 NNNCCACTGTGTGAACACTG NGC 2 0.0129 Tier III
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Other clones with same target sequence:

(none)