Construct: sgRNA BRDN0001145566
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAACTGTAGGAACTAGTCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDC42BPA (8476)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75746
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
227112907 |
- |
CDC42BPA |
NNNACTGTAGGAACTAGTCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
2870448 |
- |
EMILIN2 |
NNNACTGTAGAAACTAGTTC |
NGG |
2 |
0.4615 |
Tier II |
| 3 |
NC_000011.10 |
11 |
125083153 |
+ |
SLC37A2 |
NNNACTGTAGGAATTAGTCA |
NGG |
2 |
0.3667 |
Tier II |
| 4 |
NC_000003.12 |
3 |
29904108 |
+ |
RBMS3 |
NNNACTGTAGAAAATAGTCC |
NGG |
2 |
0.35 |
Tier II |
| 5 |
NC_000012.12 |
12 |
66191349 |
- |
IRAK3 |
NNNACTGTAGCAACTAGCCC |
NGG |
2 |
0.1837 |
Tier II |
| 6 |
NC_000012.12 |
12 |
26256045 |
- |
ITPR2-AS1 |
NNNACTGTAGGGACAAGTCC |
NGG |
2 |
0.4181 |
Tier III |
| 7 |
NC_000012.12 |
12 |
26256045 |
- |
LOC105369705 |
NNNACTGTAGGGACAAGTCC |
NGG |
2 |
0.4181 |
Tier III |
| 8 |
NC_000011.10 |
11 |
102858861 |
- |
LOC124902741 |
NNNAGTGTAGGAAATAGTCC |
NGG |
2 |
0.21 |
Tier III |
| 9 |
NC_000005.10 |
5 |
149086118 |
- |
SH3TC2-DT |
NNNTCTGTAGGAACCAGTCC |
NGG |
2 |
0.1736 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
180093951 |
+ |
Cdc42bpa |
NNNACTGTAGGAACTAGTCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000071.6 |
5 |
145008225 |
+ |
Kpna7 |
NNNACTGCAGGAACCAGTCC |
NGG |
2 |
0.2727 |
Tier II |
| 3 |
NC_000086.7 |
X |
84061666 |
- |
Dmd |
NNNACTGTAGGAACAAGTAC |
NGG |
2 |
0.2481 |
Tier II |
| 4 |
NC_000074.6 |
8 |
88725289 |
- |
Cyld |
NNNACTGGAGGAACTAGTCC |
NGA |
2 |
0.0509 |
Tier II |
| 5 |
NC_000075.6 |
9 |
62748407 |
- |
Itga11 |
NNNACTGTAGGAACTATTCC |
NGA |
2 |
0.0174 |
Tier II |
| 6 |
NC_000068.7 |
2 |
24809289 |
- |
Ehmt1 |
NNNACTGTAAGAACTTGTCC |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000068.7 |
2 |
95443597 |
- |
Gm13794 |
NNNACTGCAGGAATTAGTCC |
NGG |
2 |
0.7333 |
Tier III |
| 8 |
NC_000071.6 |
5 |
145008225 |
+ |
Gm33904 |
NNNACTGCAGGAACCAGTCC |
NGG |
2 |
0.2727 |
Tier III |
| 9 |
NC_000071.6 |
5 |
145019982 |
- |
Gm33904 |
NNNACTGCAGGAACCAGTCC |
NGG |
2 |
0.2727 |
Tier III |
| 10 |
NC_000074.6 |
8 |
26962748 |
+ |
Gm39150 |
NNNCCTGTAGGAACTTGTCC |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
