Construct: sgRNA BRDN0001145571

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGTGGTGTTCATGTGCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XYLB (9942)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77646

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 38360362 + XYLB NNNTGGTGTTCATGTGCACA NGG 0 1.0 Tier I
2 NC_000001.11 1 89666385 - LRRC8C NNNTAGTGTACATGTGCACA NGG 2 0.7429 Tier II
3 NC_000008.11 8 14533038 + SGCZ NNNTAGTGTACATGTGCACA NGG 2 0.7429 Tier II
4 NC_000006.12 6 46917868 - ADGRF5 NNNTGGGGTACATGTGCACA NGG 2 0.5042 Tier II
5 NC_000005.10 5 129589970 + ADAMTS19 NNNTGATGTTCATGTGCTCA NGG 2 0.5 Tier II
6 NC_000001.11 1 98673682 + SNX7 NNNTGCTTTTCATGTGCACA NGG 2 0.4261 Tier II
7 NC_000022.11 22 26372284 + SEZ6L NNNTGGGGTTCATGAGCACA NGG 2 0.3406 Tier II
8 NC_000004.12 4 122978794 - AFG2A NNNAGGTGTTCATGTGCTCA NGG 2 0.2381 Tier II
9 NC_000006.12 6 169247981 + THBS2 NNNTGGTGTTTGTGTGCACA NGG 2 0.2222 Tier II
10 NC_000021.9 21 45879529 + PCBP3 NNNTGGTTTTTATGTGCACA NGG 2 0.1923 Tier II
11 NC_000021.9 21 31137968 + TIAM1 NNNTGCTGTTCCTGTGCACA NGG 2 0.1794 Tier II
12 NC_000002.12 2 172892426 - RAPGEF4 NNNGGGTGTTGATGTGCACA NGG 2 0.1563 Tier II
13 NC_000023.11 X 92381337 - PCDH11X NNNTGGTGTTCATTAGCACA NGG 2 0.1544 Tier II
14 NC_000024.10 Y 5492908 - PCDH11Y NNNTGGTGTTCATTAGCACA NGG 2 0.1544 Tier II
15 NC_000007.14 7 51060148 - COBL NNNTGGTGTTCATGTGCTCA NAG 2 0.1296 Tier II
16 NC_000014.9 14 31853735 - NUBPL NNNTGGTGTACATGTGCAGA NGG 2 0.1071 Tier II
17 NC_000014.9 14 37838725 + TTC6 NNNTGGTGTACATGTGCACA NCG 2 0.0918 Tier II
18 NC_000001.11 1 230681687 - COG2 NNNTGGTTTTCATGTGCACA NCG 2 0.067 Tier II
19 NC_000016.10 16 4914113 + PPL NNNTGGTGTTCCTGTGCAGA NGG 2 0.0329 Tier II
20 NC_000002.12 2 190533973 - NEMP2 NNNTGGTGTTCGTGTGCACA NTG 2 0.0281 Tier II
21 NC_000002.12 2 166298362 - SCN9A NNNTGGGGTTCATGTGCACA NTG 2 0.0229 Tier II
22 NC_000018.10 18 54290947 - POLI NNNTGGGGTTCATGTGCACA NTG 2 0.0229 Tier II
23 NC_000021.9 21 21443395 - NCAM2 NNNTGGGGTTCATGTGCACA NTG 2 0.0229 Tier II
24 NC_000002.12 2 153669283 + GALNT13 NNNTGGTGTACATGTGCACA NGT 2 0.0138 Tier II
25 NC_000018.10 18 37435349 + CELF4 NNNTGGTGTCCATGTGCACA NGT 2 0.0086 Tier II
26 NC_000005.10 5 69175884 - CCNB1 NNNTGGTGTTCATGTGAACA NGT 2 0.0075 Tier II
27 NC_000012.12 12 18302581 + PIK3C2G NNNTGGTGTTCATGTGCACC NGT 2 0.0037 Tier II
28 NC_000013.11 13 58191468 - LINC02338 NNNTGGGGATCATGTGCACA NGG 2 0.5462 Tier III
29 NC_000011.10 11 112511499 - LINC02763 NNNTGGTTTTCATGTGAACA NGG 2 0.2917 Tier III
30 NC_000012.12 12 61667752 + LOC105369793 NNNTGGTGTTTATGTGTACA NGG 2 0.1436 Tier III
31 NC_000005.10 5 114499098 - LOC101927078 NNNTGGTGTTCAGGTGCACA NAG 2 0.0676 Tier III
32 NC_000012.12 12 58748054 + LOC100506869 NNNTGGGGTTCATGTGCACA NCG 2 0.063 Tier III
33 NC_000012.12 12 58748054 + LINC02388 NNNTGGGGTTCATGTGCACA NCG 2 0.063 Tier III
34 NC_000013.11 13 18304874 - IGSF3P1 NNNTGGTGTTCATGTGCACA NTG 1 0.039 Tier III
35 NC_000006.12 6 74565634 - LOC101928516 NNNTAGTGTTCATGTGCACA NTG 2 0.0338 Tier III
36 NC_000016.10 16 4914113 + LOC124903637 NNNTGGTGTTCCTGTGCAGA NGG 2 0.0329 Tier III
37 NC_000002.12 2 33724630 + LINC01317 NNNTGGTGTTCATGTGCAGC NGG 2 0.0284 Tier III
38 NC_000002.12 2 166298362 - SCN1A-AS1 NNNTGGGGTTCATGTGCACA NTG 2 0.0229 Tier III
39 NC_000002.12 2 91741779 + IGSF3P2 NNNTGGTGTTCATGTGCACG NGT 2 0.0123 Tier III
40 NC_000005.10 5 79557753 - LOC105379047 NNNTGGTGTCCATGTGCACA NGT 2 0.0086 Tier III
41 NC_000005.10 5 69175884 - LOC124901203 NNNTGGTGTTCATGTGAACA NGT 2 0.0075 Tier III
42 NC_000012.12 12 18302581 + LOC124902890 NNNTGGTGTTCATGTGCACC NGT 2 0.0037 Tier III
43 NC_000013.11 13 69264511 + LINC00383 NNNTGGTGTTCTTGTCCACA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 72452925 - Gm9195 NNNTGGTGTCCAGGTGCACA NGG 2 0.1391 Tier I
2 NC_000073.6 7 46302879 - Otog NNNTGGTGTTCCTGTGCACA NTG 2 0.0103 Tier I
3 NC_000078.6 12 52878468 + Akap6 NNNTGGTGTTCATGTGCACA NGG 0 1.0 Tier II
4 NC_000086.7 X 52611327 - Gpc3 NNNTAATGTTCATGTGCACA NGG 2 0.8667 Tier II
5 NC_000083.6 17 30229577 + Btbd9 NNNTAGTGCTCATGTGCACA NGG 2 0.8 Tier II
6 NC_000074.6 8 122094046 - Zfp469 NNNTGGTGCACATGTGCACA NGG 2 0.7912 Tier II
7 NC_000080.6 14 77670206 - Enox1 NNNTGGTATTCACGTGCACA NGG 2 0.7895 Tier II
8 NC_000078.6 12 36589031 + Crppa NNNTGGTATTCATGTGCTCA NGG 2 0.5 Tier II
9 NC_000071.6 5 116459492 + Srrm4 NNNTGATGTTCATGTGTACA NGG 2 0.4667 Tier II
10 NC_000082.6 16 12086964 - Shisa9 NNNTGGTGTCCATATGCACA NGG 2 0.4 Tier II
11 NC_000082.6 16 6339777 - Rbfox1 NNNTGGAGTTCATCTGCACA NGG 2 0.375 Tier II
12 NC_000081.6 15 23367763 + Cdh18 NNNTGCTGTCCATGTGCACA NGG 2 0.3636 Tier II
13 NC_000069.6 3 97650854 + Fmo5 NNNTGGTTTGCATGTGCACA NGG 2 0.3125 Tier II
14 NC_000081.6 15 37625755 + Ncald NNNTGGTGGGCATGTGCACA NGG 2 0.3095 Tier II
15 NC_000082.6 16 96382599 - Igsf5 NNNGGGTGTTCATGTGTACA NGG 2 0.2917 Tier II
16 NC_000084.6 18 61239419 + Pde6a NNNTGTTGTTCATGTGCAAA NGG 2 0.2857 Tier II
17 NC_000080.6 14 24455821 + Polr3a NNNAGGTGTTCATGAGCACA NGG 2 0.2757 Tier II
18 NC_000077.6 11 53101185 + Fstl4 NNNTGGTGTTCCTGTGCACA NGG 1 0.2632 Tier II
19 NC_000077.6 11 76166429 + Vps53 NNNTTGTGTACATGTGCACA NGG 2 0.2571 Tier II
20 NC_000074.6 8 35789663 + Gm19410 NNNTGGTGTTCATGTGCCCA NGG 1 0.1905 Tier II
21 NC_000081.6 15 85988885 - Celsr1 NNNGTGTGTTCATGTGCACA NGG 2 0.1875 Tier II
22 NC_000068.7 2 35810914 + Ttll11 NNNTGGTGTCTATGTGCACA NGG 2 0.1641 Tier II
23 NC_000076.6 10 26356385 - L3mbtl3 NNNTTGTGTCCATGTGCACA NGG 2 0.16 Tier II
24 NC_000075.6 9 21812428 - Dock6 NNNTGGTGCTCATGTGCACA NCG 2 0.0989 Tier II
25 NC_000068.7 2 104209921 + D430041D05Rik NNNTGGTGTGCATGTGCACA NGA 2 0.0347 Tier II
26 NC_000074.6 8 3406615 - Arhgef18 NNNTGGTGTGCATGTGCACA NGA 2 0.0347 Tier II
27 NC_000076.6 10 9771067 - Stxbp5 NNNTGGTGTTCATGTGAACA NGA 2 0.0324 Tier II
28 NC_000086.7 X 140980667 + Atg4a NNNTGGTGTCCATGTGCACA NTG 2 0.0208 Tier II
29 NC_000067.6 1 177146779 - Akt3 NNNTGGTGTACATGTGCACA NGC 2 0.019 Tier II
30 NC_000077.6 11 85555369 - Bcas3 NNNTGGTGTACATGTGCACA NGC 2 0.019 Tier II
31 NC_000067.6 1 87636844 - Inpp5d NNNTGGTGTTCATGCGCACA NGA 2 0.0189 Tier II
32 NC_000068.7 2 62842432 - Kcnh7 NNNTGGTGTTCATATGCACA NGC 2 0.0167 Tier II
33 NC_000076.6 10 89643541 - Scyl2 NNNTGGTGTTCATGTGCACG NGT 2 0.0123 Tier II
34 NC_000072.6 6 137101715 + Rerg NNNAGGTGTTCATGTGCACA NGC 2 0.0106 Tier II
35 NC_000085.6 19 58434789 + Gfra1 NNNTGGTGTTCCTGTGCACA NTG 2 0.0103 Tier II
36 NC_000073.6 7 30507565 - Prodh2 NNNTTGTGTTCATGTGCACA NGT 2 0.0048 Tier II
37 NC_000076.6 10 44080256 + Crybg1 NNNTGGTGTTCATGACCACA NGG 2 0.0 Tier II
38 NC_000086.7 X 52611327 - A630012P03Rik NNNTAATGTTCATGTGCACA NGG 2 0.8667 Tier III
39 NC_000074.6 8 122094046 - Gm34413 NNNTGGTGCACATGTGCACA NGG 2 0.7912 Tier III
40 NC_000071.6 5 116459492 + Srrm4os NNNTGATGTTCATGTGTACA NGG 2 0.4667 Tier III
41 NC_000071.6 5 144503793 + Gm33765 NNNTGGTGTTCATGTGTACA NGG 1 0.4667 Tier III
42 NC_000077.6 11 76166429 + Gm46252 NNNTTGTGTACATGTGCACA NGG 2 0.2571 Tier III
43 NC_000079.6 13 107130219 - Gm31452 NNNTTGTGTACATGTGCACA NGG 2 0.2571 Tier III
44 NC_000069.6 3 28779576 + 1700112D23Rik NNNTGGTGTTCCTATGCACA NGG 2 0.1974 Tier III
45 NC_000075.6 9 43947746 - Gm30373 NNNTGGGGTTTATGTGCACA NGG 2 0.181 Tier III
46 NC_000082.6 16 95237077 - Gm31641 NNNTGGTCTTCATGTGCACA NGA 2 0.0427 Tier III
47 NC_000082.6 16 95921802 + 1600002D24Rik NNNTGGTGTTCATGTACACA NGT 2 0.0161 Tier III
48 NC_000082.6 16 95921802 + Gm41508 NNNTGGTGTTCATGTACACA NGT 2 0.0161 Tier III
49 NC_000068.7 2 160234103 + Gm34805 NNNTTGTGTTCATGTGCACA NGT 2 0.0048 Tier III
50 NC_000067.6 1 9770234 - 1700034P13Rik NNNTGGTGTTCATGGGCACA NGT 2 0.0008 Tier III
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Other clones with same target sequence:

(none)