Construct: sgRNA BRDN0001145572
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCGGGAAAAGTGATTCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LCK (3932)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76411
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
32276019 |
- |
LCK |
NNNGGGAAAAGTGATTCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
55952071 |
- |
LYN |
NNNGGGAAAAGTGATTCGTG |
NAG |
2 |
0.1396 |
Tier I |
| 3 |
NC_000001.11 |
1 |
160338785 |
- |
COPA |
NNNGGAAACAGTGATTCGAG |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000007.14 |
7 |
130499540 |
+ |
MEST |
NNNGGGAAAAGTAATTCCAG |
NGG |
2 |
0.4396 |
Tier II |
| 5 |
NC_000010.11 |
10 |
10731188 |
- |
CELF2 |
NNNGGAAAAAGTGATTCGAC |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000002.12 |
2 |
40284741 |
- |
SLC8A1 |
NNNGGGAAAAGAGATTAGAG |
NGG |
2 |
0.3733 |
Tier II |
| 7 |
NC_000023.11 |
X |
132407981 |
- |
MBNL3 |
NNNGGGAAAAGAGATTAGAG |
NGG |
2 |
0.3733 |
Tier II |
| 8 |
NC_000006.12 |
6 |
165768716 |
- |
PDE10A |
NNNGGGAAAAGTGATTTAAG |
NGG |
2 |
0.3231 |
Tier II |
| 9 |
NC_000014.9 |
14 |
75862576 |
+ |
TTLL5 |
NNNGGGAAAAGTGATCTGAG |
NGG |
2 |
0.3111 |
Tier II |
| 10 |
NC_000009.12 |
9 |
87514440 |
- |
DAPK1 |
NNNGGGAAAAGTGATTCAAC |
NGG |
2 |
0.2967 |
Tier II |
| 11 |
NC_000002.12 |
2 |
102438530 |
- |
IL18RAP |
NNNGGGAAAAGTGGTGCGAG |
NGG |
2 |
0.1615 |
Tier II |
| 12 |
NC_000007.14 |
7 |
37202265 |
+ |
ELMO1 |
NNNGGGAAAAGTGATTCGAT |
NGA |
2 |
0.0486 |
Tier II |
| 13 |
NC_000007.14 |
7 |
3789743 |
+ |
SDK1 |
NNNGGGAAAAGGGATTGGAG |
NGG |
2 |
0.0294 |
Tier II |
| 14 |
NC_000005.10 |
5 |
167897992 |
+ |
TENM2 |
NNNGGGCAAAGTGATTGGAG |
NGG |
2 |
0.0257 |
Tier II |
| 15 |
NC_000002.12 |
2 |
165603852 |
- |
CSRNP3 |
NNNGGGAAAAGTGATTGGAG |
NAG |
2 |
0.0153 |
Tier II |
| 16 |
NC_000011.10 |
11 |
55636183 |
- |
OR4P4 |
NNNGGGAAAAGTGATTTGAG |
NGT |
2 |
0.0075 |
Tier II |
| 17 |
NC_000015.10 |
15 |
53198565 |
- |
LOC107983981 |
NNNAGGAAAAGTGATTCCAG |
NGG |
2 |
0.4286 |
Tier III |
| 18 |
NC_000023.11 |
X |
132407981 |
- |
RAP2C-AS1 |
NNNGGGAAAAGAGATTAGAG |
NGG |
2 |
0.3733 |
Tier III |
| 19 |
NC_000014.9 |
14 |
106237020 |
- |
IGH |
NNNGGGAAAACTGATTCAAG |
NGG |
2 |
0.2967 |
Tier III |
| 20 |
NC_000009.12 |
9 |
85285804 |
- |
LOC105376119 |
NNNGGGAAAAGTCATTCCAG |
NGG |
2 |
0.2005 |
Tier III |
| 21 |
NC_000013.11 |
13 |
78035941 |
- |
LINC00446 |
NNNGGGAAAAGTGAAGCGAG |
NGG |
2 |
0.2004 |
Tier III |
| 22 |
NC_000023.11 |
X |
39931870 |
+ |
LOC105373180 |
NNNGGGAAAACTGATTAGAG |
NGG |
2 |
0.2 |
Tier III |
| 23 |
NC_000003.12 |
3 |
8223319 |
+ |
LMCD1-AS1 |
NNNGGGAAAAGTGATTCAAG |
NGT |
2 |
0.0112 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
129556333 |
+ |
Lck |
NNNGGGAAAAGTGATACGAG |
NGG |
1 |
0.9091 |
Tier I |
| 2 |
NC_000070.6 |
4 |
3746816 |
- |
Lyn |
NNNGGGAAAAGTGATGCGAG |
NAG |
2 |
0.0897 |
Tier I |
| 3 |
NC_000069.6 |
3 |
126405667 |
+ |
Arsj |
NNNGGGAAAAGTGATTCTAG |
NGG |
1 |
0.6667 |
Tier II |
| 4 |
NC_000071.6 |
5 |
147597797 |
- |
Flt1 |
NNNGGGAAAAGTGATTTGAA |
NGG |
2 |
0.4375 |
Tier II |
| 5 |
NC_000071.6 |
5 |
124446869 |
- |
Kmt5a |
NNNGGGAAAACTGATTCGAA |
NGG |
2 |
0.4018 |
Tier II |
| 6 |
NC_000068.7 |
2 |
114139048 |
- |
Aqr |
NNNGGGTAAAGTGATTAGAG |
NGG |
2 |
0.2042 |
Tier II |
| 7 |
NC_000071.6 |
5 |
151288705 |
- |
Stard13 |
NNNGTGAAAAGTGGTTCGAG |
NGG |
2 |
0.14 |
Tier II |
| 8 |
NC_000082.6 |
16 |
52161912 |
+ |
Cblb |
NNNGGGAAAAGTGACTCCAG |
NGG |
2 |
0.1299 |
Tier II |
| 9 |
NC_000070.6 |
4 |
57502938 |
+ |
Pakap |
NNNGGGAAAAGTGATTCCAG |
NAG |
2 |
0.1235 |
Tier II |
| 10 |
NC_000070.6 |
4 |
108167020 |
- |
Echdc2 |
NNNGTGAAAATTGATTCGAG |
NGG |
2 |
0.1154 |
Tier II |
| 11 |
NC_000075.6 |
9 |
28261125 |
- |
Opcml |
NNNGGGAAAAGTGATTTGAG |
NGA |
2 |
0.0324 |
Tier II |
| 12 |
NC_000075.6 |
9 |
57246883 |
+ |
Trcg1 |
NNNGGGAAAAGTGAGTCCAG |
NGG |
2 |
0.0238 |
Tier II |
| 13 |
NC_000084.6 |
18 |
69607774 |
- |
Tcf4 |
NNNGGGAAAAGTGAGTTGAG |
NGG |
2 |
0.0233 |
Tier II |
| 14 |
NC_000069.6 |
3 |
126405667 |
+ |
Gm35351 |
NNNGGGAAAAGTGATTCTAG |
NGG |
1 |
0.6667 |
Tier III |
| 15 |
NC_000070.6 |
4 |
3508093 |
+ |
Gm11787 |
NNNGGGAAAAGTGATGCGAG |
NAG |
2 |
0.0897 |
Tier III |
Other clones with same target sequence:
(none)
