Construct: sgRNA BRDN0001145574

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTCTCCCAGTCATCCCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTK6 (5753)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76492

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 63533681 + PTK6 NNNTCCCAGTCATCCCAATG NGG 0 1.0 Tier I
2 NC_000005.10 5 53051512 + ITGA2 NNNTCCCAGACATCCCAATA NGG 2 0.8036 Tier I
3 NC_000020.11 20 18726607 - DTD1 NNNTCCTAGTCATCCCAATG NGG 1 0.8125 Tier II
4 NC_000008.11 8 11112770 + XKR6 NNNTCCCAGTCAACCTAATG NGG 2 0.213 Tier II
5 NC_000023.11 X 17433944 + NHS NNNTCCCACTCATACCAATG NGG 2 0.1885 Tier II
6 NC_000012.12 12 23640289 - SOX5 NNNTCCCAGTCATCCCAATT NAG 2 0.1815 Tier II
7 NC_000003.12 3 124162237 + KALRN NNNTCCCAGTCTTCCTAATG NGG 2 0.1026 Tier II
8 NC_000018.10 18 25089452 + ZNF521 NNNTCCCAGTCCTCCCCATG NGG 2 0.0464 Tier II
9 NC_000001.11 1 237155727 - RYR2 NNNTCCCATTCATCCCAATG NGA 2 0.037 Tier II
10 NC_000016.10 16 3659348 - DNASE1 NNNTCCCAGTCATCCCAGTG NTG 2 0.0156 Tier II
11 NC_000016.10 16 3659348 - TRAP1 NNNTCCCAGTCATCCCAGTG NTG 2 0.0156 Tier II
12 NC_000009.12 9 135499206 + PIERCE1 NNNTCCCAGTCATCTCAATG NGA 2 0.0046 Tier II
13 NC_000002.12 2 133965051 - LOC105373627 NNNTCCCGGTCATCCCCATG NGG 2 0.1294 Tier III
14 NC_000011.10 11 119913607 + LOC105369526 NNNTCCCAGCCATCCCACTG NGG 2 0.1016 Tier III
15 NC_000020.11 20 10136137 + SNAP25-AS1 NNNTCCCAGTCTTCCCAAAG NGG 2 0.0952 Tier III
16 NC_000023.11 X 103718997 - GLRA4 NNNTCCCATTCATCCCAATG NGC 2 0.0119 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 181199207 + Ptk6 NNNTCCCAGTCATCCCAGTG NGG 1 0.4 Tier I
2 NC_000072.6 6 116516300 + Olfr212 NNNTCCCAGTCATCCTACTG NGG 2 0.0586 Tier I
3 NC_000072.6 6 116540976 + Olfr213 NNNTCCCAGTCATCCTACTG NGG 2 0.0586 Tier I
4 NC_000085.6 19 18961762 + Rorb NNNTCCCAGTCATTCAAATG NGG 2 0.7333 Tier II
5 NC_000067.6 1 22379155 - Rims1 NNNTCCCAGTCATTCCAATG NGG 1 0.7333 Tier II
6 NC_000075.6 9 41125321 + Ubash3b NNNTCCCAGTCATCCAAATC NGG 2 0.4286 Tier II
7 NC_000071.6 5 151387434 + Stard13 NNNTCACAGTCATCCCAACG NGG 2 0.2562 Tier II
8 NC_000078.6 12 84611153 + Abcd4 NNNTCTCAGTGATCCCAATG NGG 2 0.2321 Tier II
9 NC_000079.6 13 96096570 + Sv2c NNNTCTCAGTCATCACAATG NGG 2 0.2063 Tier II
10 NC_000067.6 1 104786985 - Cdh20 NNNTCCCAGTCCTCCCAATT NGG 2 0.1842 Tier II
11 NC_000070.6 4 57204517 + Ptpn3 NNNCCCCAGTCCTCCCAATG NGG 2 0.1703 Tier II
12 NC_000070.6 4 9519390 + Asph NNNTCCCAGTCATCCCTTTG NGG 2 0.0667 Tier II
13 NC_000067.6 1 174840120 - Grem2 NNNTCCCAGTCAGCCCACTG NGG 2 0.0497 Tier II
14 NC_000079.6 13 24469719 + Cmah NNNCCCCAGTCATCCCAATG NGA 2 0.0449 Tier II
15 NC_000067.6 1 127639831 - Tmem163 NNNTCCCAGTCCTCCGAATG NGG 2 0.0405 Tier II
16 NC_000073.6 7 143007645 + Tspan32 NNNTCCCAGTCATCTGAATG NGG 2 0.0103 Tier II
17 NC_000073.6 7 5027620 - Zfp865 NNNACCCAGTCATCCCAATG NGT 2 0.0077 Tier II
18 NC_000070.6 4 120519952 + Scmh1 NNNTCCCAGTCATCTCAATG NTG 2 0.0026 Tier II
19 NC_000086.7 X 164178138 - Ace2 NNNTCCCAGTCATCCCAATG NAT 2 0.0 Tier II
20 NC_000076.6 10 90959126 + Anks1b NNNTCCCAGTCATGGCAATG NGG 2 0.0 Tier II
21 NC_000071.6 5 151387434 + 1700028E10Rik NNNTCACAGTCATCCCAACG NGG 2 0.2562 Tier III
22 NC_000068.7 2 143457635 - Gm33599 NNNTCCCAGTCTTCTCAATG NGG 2 0.0222 Tier III
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Other clones with same target sequence:

(none)