Construct: sgRNA BRDN0001145577
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTTCACACAACGTACTGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRPK2 (6733)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75599
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
105160538 |
+ |
SRPK2 |
NNNTCACACAACGTACTGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
111149933 |
- |
SLC16A10 |
NNNTCACACAAAGTAATGGG |
NGG |
2 |
0.7143 |
Tier II |
| 3 |
NC_000002.12 |
2 |
211625804 |
+ |
ERBB4 |
NNNTCACACAAAATACTGGG |
NGG |
2 |
0.6593 |
Tier II |
| 4 |
NC_000008.11 |
8 |
62863497 |
+ |
NKAIN3 |
NNNTCAGACAACGTACTGGG |
NGG |
1 |
0.4706 |
Tier II |
| 5 |
NC_000004.12 |
4 |
57065139 |
+ |
IGFBP7 |
NNNTCACAAAACGTACTGGG |
NAG |
2 |
0.2222 |
Tier II |
| 6 |
NC_000020.11 |
20 |
15349145 |
- |
MACROD2 |
NNNTAACACAACGCACTGGG |
NGG |
2 |
0.1633 |
Tier II |
| 7 |
NC_000005.10 |
5 |
155845751 |
+ |
SGCD |
NNNTCACACAACCTCCTGGG |
NGG |
2 |
0.1148 |
Tier II |
| 8 |
NC_000005.10 |
5 |
81623675 |
+ |
SSBP2 |
NNNTCACACAATGGACTGGG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000008.11 |
8 |
96328260 |
- |
PTDSS1 |
NNNTCACACAGCGGACTGGG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000008.11 |
8 |
62863497 |
+ |
SRPK2P |
NNNTCAGACAACGTACTGGG |
NGG |
1 |
0.4706 |
Tier III |
| 11 |
NC_000008.11 |
8 |
62863497 |
+ |
LOC112268019 |
NNNTCAGACAACGTACTGGG |
NGG |
1 |
0.4706 |
Tier III |
| 12 |
NC_000013.11 |
13 |
86846759 |
+ |
LOC105370300 |
NNNGCACACAACGTACTGTG |
NGG |
2 |
0.4167 |
Tier III |
| 13 |
NC_000010.11 |
10 |
3245415 |
+ |
LINC02668 |
NNNTCACCCAACGTACAGGG |
NGG |
2 |
0.2286 |
Tier III |
| 14 |
NC_000006.12 |
6 |
111243617 |
+ |
MFSD4B-DT |
NNNTCACACAACGTTTTGGG |
NGG |
2 |
0.0615 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
23540406 |
+ |
Srpk2 |
NNNTCACACAACGTACTGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000068.7 |
2 |
156024643 |
+ |
Fer1l4 |
NNNTCACACATCATACTGGG |
NGG |
2 |
0.284 |
Tier I |
| 3 |
NC_000082.6 |
16 |
96678630 |
+ |
Dscam |
NNNTCACACAACGTACAGGG |
NGG |
1 |
0.5333 |
Tier II |
| 4 |
NC_000083.6 |
17 |
30988661 |
- |
Umodl1 |
NNNACACACAACGTAATGGG |
NGG |
2 |
0.4762 |
Tier II |
| 5 |
NC_000071.6 |
5 |
105775984 |
+ |
Lrrc8d |
NNNTCACACAATGTACTGGC |
NGG |
2 |
0.2308 |
Tier II |
| 6 |
NC_000070.6 |
4 |
126743829 |
+ |
Ncdn |
NNNTCACAGAACGCACTGGG |
NGG |
2 |
0.1769 |
Tier II |
Other clones with same target sequence:
(none)
