Construct: sgRNA BRDN0001145578

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGTGGGCACTCCACCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAG3 (53632)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77295

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 218830278 - PRKAG3 NNNTGGGCACTCCACCAACA NGG 0 1.0 Tier I
2 NC_000002.12 2 178569758 - TTN NNNTGTGCACTCCACCAACA NGA 2 0.0463 Tier I
3 NC_000019.10 19 17439232 - TMEM221 NNNTGGGTATTCCACCAACA NGG 2 0.8235 Tier II
4 NC_000014.9 14 92344828 + SLC24A4 NNNTGGGCCCTCCACAAACA NGG 2 0.5714 Tier II
5 NC_000011.10 11 32892829 + QSER1 NNNTGGGAAATCCACCAACA NGG 2 0.5633 Tier II
6 NC_000004.12 4 6287327 - WFS1 NNNTGGGCACCCTACCAACA NGG 2 0.4667 Tier II
7 NC_000012.12 12 104631363 - CHST11 NNNTGGGCACAGCACCAACA NGG 2 0.3333 Tier II
8 NC_000022.11 22 39478388 - MGAT3 NNNTGGGCACTCCTCCAACT NGG 2 0.32 Tier II
9 NC_000006.12 6 168503691 - SMOC2 NNNTGGGCTCTGCACCAACA NGG 2 0.2667 Tier II
10 NC_000001.11 1 54305005 + SSBP3 NNNTGTGCACTCCACCAGCA NGG 2 0.2667 Tier II
11 NC_000007.14 7 31811934 + PDE1C NNNTGGGCACTCCACAAAGA NGG 2 0.125 Tier II
12 NC_000017.11 17 50064427 - ITGA3 NNNTGGGCCCTCCACCCACA NGG 2 0.1008 Tier II
13 NC_000013.11 13 31297200 + B3GLCT NNNTGGACACTCCATCAACA NGG 2 0.0667 Tier II
14 NC_000007.14 7 4846041 + RADIL NNNTGGGCACGCCACCAACA NCG 2 0.0429 Tier II
15 NC_000017.11 17 47000590 + LRRC37A2 NNNTGGGCACTCCACCATCA NGT 2 0.0081 Tier II
16 NC_000011.10 11 18742559 - PTPN5 NNNTGGGCACTCCAGGAACA NGG 2 0.0077 Tier II
17 NC_000007.14 7 18511716 + HDAC9 NNNTGGGCACTCAACCAACA NGT 2 0.0062 Tier II
18 NC_000019.10 19 51962322 - ZNF350-AS1 NNNTGGGCGCTCCACCAACC NGG 2 0.1515 Tier III
19 NC_000014.9 14 22337117 + TRA NNNTGGGCAATCCATCAACA NGG 2 0.0578 Tier III
20 NC_000002.12 2 178569758 - TTN-AS1 NNNTGTGCACTCCACCAACA NGA 2 0.0463 Tier III
21 NC_000023.11 X 10990670 + HCCS-DT NNNTGGGCCCTCCACCAACA NTG 2 0.0223 Tier III
22 NC_000017.11 17 47000590 + LOC101927060 NNNTGGGCACTCCACCATCA NGT 2 0.0081 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 130897420 - Plekha1 NNNTGGGCACACCACCAACA NTG 2 0.0292 Tier I
2 NC_000079.6 13 47237496 - Rnf144b NNNAGGGCACTCCACCAACA NTG 2 0.0186 Tier I
3 NC_000072.6 6 43309245 + Nobox NNNTAGGCACTCCACAAACA NGG 2 0.8667 Tier II
4 NC_000076.6 10 71038358 - Bicc1 NNNTGAGCACCCCACCAACA NGG 2 0.6667 Tier II
5 NC_000079.6 13 40895803 + Gcnt2 NNNGGGGCAATCCACCAACA NGG 2 0.5417 Tier II
6 NC_000068.7 2 102816353 + Cd44 NNNTGGGAACACCACCAACA NGG 2 0.4875 Tier II
7 NC_000075.6 9 65230488 + Parp16 NNNTGGGCACTGCACAAACA NGG 2 0.4444 Tier II
8 NC_000068.7 2 24825266 + Ehmt1 NNNTAGGCACTGCACCAACA NGG 2 0.3852 Tier II
9 NC_000071.6 5 67420928 - Bend4 NNNTGGGCACCCCACCAAAA NGG 2 0.2857 Tier II
10 NC_000073.6 7 28761494 + Nfkbib NNNTGAGCACTCCACCAACC NGG 2 0.2273 Tier II
11 NC_000082.6 16 12264008 + Shisa9 NNNTGGGCACTTAACCAACA NGG 2 0.2071 Tier II
12 NC_000075.6 9 27150835 + Jam3 NNNTGGGCACTCCACACACA NGG 2 0.1765 Tier II
13 NC_000080.6 14 55683061 - Gmpr2 NNNTGGGCACTCCACACACA NGG 2 0.1765 Tier II
14 NC_000082.6 16 97742991 + Ripk4 NNNTGGGCAGGCCACCAACA NGG 2 0.1556 Tier II
15 NC_000086.7 X 162048174 - Nhs NNNTGGGCACTCAACCAGCA NGG 2 0.1538 Tier II
16 NC_000069.6 3 27184530 - Nceh1 NNNTGGGCACTCCACCCACG NGG 2 0.1349 Tier II
17 NC_000068.7 2 49314797 - Mbd5 NNNTGGGCACTCCACAAAGA NGG 2 0.125 Tier II
18 NC_000078.6 12 85952001 - Ttll5 NNNTGGGCACTGCACCCACA NGG 2 0.0784 Tier II
19 NC_000084.6 18 65464356 + Malt1 NNNTGGGCACTCCACCAGCA NGC 2 0.0089 Tier II
20 NC_000083.6 17 25928535 + Pigq NNNTGGGCACTCCACCCACA NGC 2 0.0039 Tier II
21 NC_000079.6 13 48725522 + Gm36784 NNNTGGGCACTCCACAAACA NGG 1 1.0 Tier III
22 NC_000078.6 12 25175294 + Gm36287 NNNTTGGCACTCCACCATCA NGG 2 0.15 Tier III
23 NC_000079.6 13 47237496 - Gm52039 NNNAGGGCACTCCACCAACA NTG 2 0.0186 Tier III
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Other clones with same target sequence:

(none)