Construct: sgRNA BRDN0001145579
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGCAGGCATTCAAAAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IKBKB (3551)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76090
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
42314332 |
+ |
IKBKB |
NNNGCAGGCATTCAAAAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
148767728 |
+ |
CUL1 |
NNNGCAGCCATTGAAAAGTG |
NGG |
2 |
0.0839 |
Tier I |
| 3 |
NC_000008.11 |
8 |
92884215 |
+ |
TRIQK |
NNNGTAGGTATTCAAAAGTG |
NGG |
2 |
0.5568 |
Tier II |
| 4 |
NC_000006.12 |
6 |
142823244 |
- |
HIVEP2 |
NNNGCAGGCTTTCTAAAGTG |
NGG |
2 |
0.4706 |
Tier II |
| 5 |
NC_000006.12 |
6 |
101498542 |
- |
GRIK2 |
NNNGCAAGCATTCAAAAGTC |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000002.12 |
2 |
148021669 |
+ |
MBD5 |
NNNGAAGGCACTCAAAAGTG |
NGG |
2 |
0.381 |
Tier II |
| 7 |
NC_000021.9 |
21 |
38565006 |
- |
ERG |
NNNGCAGGAAGTCAAAAGTG |
NGG |
2 |
0.3429 |
Tier II |
| 8 |
NC_000023.11 |
X |
135986508 |
+ |
SLC9A6 |
NNNTCAGGCATACAAAAGTG |
NGG |
2 |
0.2909 |
Tier II |
| 9 |
NC_000004.12 |
4 |
98018692 |
- |
STPG2 |
NNNGCAGTCATTCAAAAGTC |
NGG |
2 |
0.2679 |
Tier II |
| 10 |
NC_000004.12 |
4 |
118163090 |
+ |
NDST3 |
NNNGCAGTCATTCAAAAGTC |
NGG |
2 |
0.2679 |
Tier II |
| 11 |
NC_000008.11 |
8 |
14091499 |
- |
SGCZ |
NNNGCAGTCATTCAAAAGTC |
NGG |
2 |
0.2679 |
Tier II |
| 12 |
NC_000009.12 |
9 |
92082914 |
- |
SPTLC1 |
NNNGCAGTCATTCAAAAGTC |
NGG |
2 |
0.2679 |
Tier II |
| 13 |
NC_000010.11 |
10 |
115327425 |
+ |
ATRNL1 |
NNNGCAGTCATTCAAAAGTC |
NGG |
2 |
0.2679 |
Tier II |
| 14 |
NC_000002.12 |
2 |
158328688 |
- |
CCDC148 |
NNNGCAGTCATTAAAAAGTG |
NGG |
2 |
0.2404 |
Tier II |
| 15 |
NC_000004.12 |
4 |
69085144 |
- |
UGT2B7 |
NNNGCAGTCATTAAAAAGTG |
NGG |
2 |
0.2404 |
Tier II |
| 16 |
NC_000005.10 |
5 |
14412852 |
+ |
TRIO |
NNNGCAGGCAATCAAAAGCG |
NGG |
2 |
0.2069 |
Tier II |
| 17 |
NC_000002.12 |
2 |
42364926 |
- |
COX7A2L |
NNNGCAGGCAGGCAAAAGTG |
NGG |
2 |
0.2 |
Tier II |
| 18 |
NC_000010.11 |
10 |
10582201 |
- |
CELF2 |
NNNGCAGGGATTCACAAGTG |
NGG |
2 |
0.1688 |
Tier II |
| 19 |
NC_000002.12 |
2 |
182227895 |
+ |
PDE1A |
NNNGCAGTCATTCCAAAGTG |
NGG |
2 |
0.1339 |
Tier II |
| 20 |
NC_000013.11 |
13 |
25858157 |
+ |
ATP8A2 |
NNNGCAGGCATTTAAAAGTG |
NTG |
2 |
0.0273 |
Tier II |
| 21 |
NC_000002.12 |
2 |
159372231 |
+ |
BAZ2B |
NNNGCAGGCATTCAAAAATG |
NGT |
2 |
0.0112 |
Tier II |
| 22 |
NC_000012.12 |
12 |
88173728 |
- |
TMTC3 |
NNNGCAGGCATTCAAAAATG |
NGT |
2 |
0.0112 |
Tier II |
| 23 |
NC_000002.12 |
2 |
162391142 |
- |
KCNH7 |
NNNGCAGGCATTCAAGAGTG |
NTG |
2 |
0.0075 |
Tier II |
| 24 |
NC_000013.11 |
13 |
100717854 |
- |
NALCN-AS1 |
NNNGTAGGCATTCAAAAATG |
NGG |
2 |
0.4406 |
Tier III |
| 25 |
NC_000004.12 |
4 |
118163090 |
+ |
LOC107986307 |
NNNGCAGTCATTCAAAAGTC |
NGG |
2 |
0.2679 |
Tier III |
| 26 |
NC_000002.12 |
2 |
66023977 |
+ |
LINC02934 |
NNNGCAAGCATTCAAAAGTG |
NAG |
2 |
0.2593 |
Tier III |
| 27 |
NC_000005.10 |
5 |
142325527 |
+ |
SPRY4-AS1 |
NNNGCAGGCAGTCAAAAGGG |
NGG |
2 |
0.1 |
Tier III |
| 28 |
NC_000014.9 |
14 |
101330068 |
+ |
LOC124903387 |
NNNGCAGGCATTAAAGAGTG |
NGG |
2 |
0.074 |
Tier III |
| 29 |
NC_000003.12 |
3 |
187293488 |
- |
LOC101929130 |
NNNGCTGGCATTCAAAAGTG |
NGA |
2 |
0.0496 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
139397715 |
+ |
Stpg2 |
NNNACAGACATTCAAAAGTG |
NGG |
2 |
0.9 |
Tier II |
| 2 |
NC_000072.6 |
6 |
146238961 |
+ |
Itpr2 |
NNNGCAGGCATTCAAAATTG |
NGG |
1 |
0.6667 |
Tier II |
| 3 |
NC_000067.6 |
1 |
156832264 |
- |
Ralgps2 |
NNNACAGGCATTCAAAATTG |
NGG |
2 |
0.6 |
Tier II |
| 4 |
NC_000079.6 |
13 |
18855500 |
+ |
Vps41 |
NNNGAAGGTATTCAAAAGTG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000071.6 |
5 |
110765511 |
+ |
Ep400 |
NNNGCAGGCATGCAAAAGTA |
NGG |
2 |
0.4688 |
Tier II |
| 6 |
NC_000068.7 |
2 |
48950160 |
+ |
Orc4 |
NNNGAAGGCACTCAAAAGTG |
NGG |
2 |
0.381 |
Tier II |
| 7 |
NC_000068.7 |
2 |
48950160 |
+ |
Mbd5 |
NNNGAAGGCACTCAAAAGTG |
NGG |
2 |
0.381 |
Tier II |
| 8 |
NC_000067.6 |
1 |
74093059 |
- |
Tns1 |
NNNGCAGGCATTGAGAAGTG |
NGG |
2 |
0.0886 |
Tier II |
| 9 |
NC_000070.6 |
4 |
82391411 |
- |
Nfib |
NNNGCAGGCCTTCAAAAGTG |
NAG |
2 |
0.0864 |
Tier II |
| 10 |
NC_000073.6 |
7 |
100897972 |
- |
Arhgef17 |
NNNGCAAGCATTCAAAAGTG |
NGA |
2 |
0.0694 |
Tier II |
| 11 |
NC_000079.6 |
13 |
18135298 |
+ |
Pou6f2 |
NNNGCAGGCATTCAAAAGTA |
NGA |
2 |
0.0651 |
Tier II |
| 12 |
NC_000074.6 |
8 |
25084698 |
+ |
Plekha2 |
NNNGCAGGCATTCAAACGGG |
NGG |
2 |
0.0441 |
Tier II |
| 13 |
NC_000085.6 |
19 |
4266320 |
- |
Ssh3 |
NNNGCAGGCATTGAACAGTG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000072.6 |
6 |
146238961 |
+ |
Gm35596 |
NNNGCAGGCATTCAAAATTG |
NGG |
1 |
0.6667 |
Tier III |
| 15 |
NC_000084.6 |
18 |
33496976 |
- |
Gm41684 |
NNNGCAGGAATTCAAAACTG |
NGG |
2 |
0.4082 |
Tier III |
Other clones with same target sequence:
(none)
