Construct: sgRNA BRDN0001145580
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGAGAAGAGGGATAATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- VRK3 (51231)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75550
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
50009306 |
+ |
VRK3 |
NNNGAGAAGAGGGATAATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
28822974 |
+ |
OPRD1 |
NNNAGGAAGAGGGATAATCG |
NGG |
2 |
0.648 |
Tier II |
| 3 |
NC_000002.12 |
2 |
143550034 |
+ |
ARHGAP15 |
NNNGAGAAGAGAGATAATAG |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000004.12 |
4 |
185291203 |
+ |
SNX25 |
NNNGAGAAGAGGGTTAATTG |
NGG |
2 |
0.2462 |
Tier II |
| 5 |
NC_000004.12 |
4 |
41158084 |
+ |
APBB2 |
NNNGAGAAGTGGGATATTCG |
NGG |
2 |
0.1176 |
Tier II |
| 6 |
NC_000018.10 |
18 |
6272169 |
+ |
L3MBTL4 |
NNNGAAAAGAGGGATAATCG |
NCG |
2 |
0.1071 |
Tier II |
| 7 |
NC_000009.12 |
9 |
109498975 |
- |
PTPN3 |
NNNGAGCAGAGGGCTAATCG |
NGG |
2 |
0.0938 |
Tier II |
| 8 |
NC_000002.12 |
2 |
74428441 |
- |
RTKN |
NNNGAGAAGAGGGATACTGG |
NGG |
2 |
0.0221 |
Tier II |
| 9 |
NC_000012.12 |
12 |
67480361 |
- |
LOC105369812 |
NNNGAGAAGAGAGATAATTG |
NGG |
2 |
0.4308 |
Tier III |
| 10 |
NC_000006.12 |
6 |
48557191 |
+ |
LOC107986602 |
NNNGAGAAGTGGGATAATAG |
NGG |
2 |
0.3782 |
Tier III |
| 11 |
NC_000020.11 |
20 |
21682288 |
- |
LINC01726 |
NNNGAGAAGAGGGAGAATTG |
NGG |
2 |
0.0231 |
Tier III |
| 12 |
NC_000018.10 |
18 |
47405951 |
- |
MIR4527HG |
NNNGAGAAGAGGGAGAATCC |
NGG |
2 |
0.0214 |
Tier III |
| 13 |
NC_000007.14 |
7 |
26043846 |
- |
LOC105375199 |
NNNGAGAAGAGGGATCATGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
119458303 |
- |
Ino80 |
NNNGAGAAGAGGAATAATCT |
NGG |
2 |
0.6462 |
Tier II |
| 2 |
NC_000067.6 |
1 |
130184689 |
+ |
Thsd7b |
NNNGAGAAGAGGGATAACCG |
NGG |
1 |
0.4286 |
Tier II |
| 3 |
NC_000073.6 |
7 |
4476301 |
+ |
Eps8l1 |
NNNTAGAAGAGGGATAATTG |
NGG |
2 |
0.1678 |
Tier II |
| 4 |
NC_000067.6 |
1 |
126823371 |
+ |
Nckap5 |
NNNGAGAAGAGGGATAATGG |
NGG |
1 |
0.125 |
Tier II |
| 5 |
NC_000085.6 |
19 |
47517229 |
+ |
Stn1 |
NNNGAGAAGAGGTATAAGCG |
NGG |
2 |
0.1 |
Tier II |
| 6 |
NC_000074.6 |
8 |
93831331 |
- |
Gnao1 |
NNNGAGAAGAGGGAAAATGG |
NGG |
2 |
0.0724 |
Tier II |
| 7 |
NC_000081.6 |
15 |
4799877 |
- |
C6 |
NNNGAGAAGGGGGATAATGG |
NGG |
2 |
0.0694 |
Tier II |
| 8 |
NC_000072.6 |
6 |
84067424 |
- |
Dysf |
NNNGAGAAGAGGGATAATCA |
NTG |
2 |
0.0365 |
Tier II |
| 9 |
NC_000068.7 |
2 |
38065375 |
- |
Dennd1a |
NNNGAGAAGAGGAATAATCG |
NTG |
2 |
0.036 |
Tier II |
| 10 |
NC_000072.6 |
6 |
62254238 |
+ |
Ccser1 |
NNNGAGAAGAGGGATAATAG |
NGA |
2 |
0.0298 |
Tier II |
| 11 |
NC_000073.6 |
7 |
82388216 |
- |
Adamtsl3 |
NNNGAGAAGAGGGATGATGG |
NGG |
2 |
0.024 |
Tier II |
| 12 |
NC_000086.7 |
X |
168087223 |
- |
Frmpd4 |
NNNGAGAAGAGGGATAATAG |
NTG |
2 |
0.0167 |
Tier II |
| 13 |
NC_000081.6 |
15 |
89475433 |
+ |
Arsa |
NNNGAGAAGAGGGAGAATGG |
NGG |
2 |
0.0063 |
Tier II |
| 14 |
NC_000083.6 |
17 |
3114292 |
- |
A230045G11Rik |
NNNGAGCAGAGGGATAATTG |
NGG |
2 |
0.2019 |
Tier III |
| 15 |
NC_000072.6 |
6 |
31143093 |
+ |
Lncpint |
NNNGAGAAGAGGGTGAATCG |
NGG |
2 |
0.0267 |
Tier III |
| 16 |
NC_000067.6 |
1 |
120872636 |
- |
Gm34584 |
NNNGAGAAGAGGGATAATGG |
NGT |
2 |
0.002 |
Tier III |
Other clones with same target sequence:
(none)
